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1、組胚切片報告組織學與胚胎學實驗報指定主題:實驗上皮組織基本組織1報告主題:假復層纖毛柱狀上皮主題屬性:指定標本號:27#染色:HE染色教學要求:掌握假復層纖毛柱狀上皮的光鏡結(jié)構(gòu)特征纖毛Cilium杯狀細胞Goblet cell 結(jié)締組織Conn ectiveTissue_*i光鏡下可見假復層纖毛柱狀上皮的各種上皮細胞界限不清,細胞核染藍紫色,高矮不一,其中柱狀細胞最多,游離面有大量纖毛,柱狀細胞之前常夾有杯狀細胞,其底部狹窄,頂部膨大,細胞核深染。所有細胞的基底膜均附著在基膜上,即使細胞核高低不齊排列,形似復層,實為單層。PQOL PThe n visible p seudostratifie
2、d ciliated colu mnar ep ithelium of ep ithelial cell li ne is not clear, the nu cleus dyed purple blue, height is differ, the colu mnar cells most, free surface has a lot of ciliated colu mnar cells, before ofte n clip has goblet cell, its bottom is n arrow, the top swolle n, with hyperchromatic nuc
3、lei. All the cells of the basement membrane are attached on the baseme nt membra ne, whose nu cleus are irregular, and the sha pe see as compound layer, and it is a sin gleZq9o1。實驗二結(jié)締組織基本組織2報告主題:骨單位主題屬性:指定標本號:6#材料:骨切片染色:Schmorl氏法塊染教學要求:掌握骨組織的結(jié)構(gòu)中央管Cen tral canal骨板Oste on lamella, *4?Pl間骨板In terstitia
4、l lamella圖2骨單位(骨切片,Schmorl氏法塊染,40X100)Figure 2 osteon (osseous smear,Schmorl stained,4000) isHjt。X骨單位位于內(nèi)外環(huán)骨板之間,數(shù)量最多,中央為圓心的中央管,中央管與穿通管相通,數(shù)層同心圓排列的骨板圍繞中央管。在骨單位之間,有一些不規(guī)則排列的間骨板, 其中無血管通道,在骨板之間或骨板中可見黃色小腔,即骨陷窩。BiW7gOste on is located in the in side and outside ring bone pl ate betwee n, the largest nu mber,
5、 the cen tral to the right of the cen tral tube, the cen tral tube and pi erced tube are in terl in ked, layers of concen tric arran geme nt of the bone p late around the cen tral can al. There are some in terstitial lamellae betwee n oste on, which has no vascular cha nn els, in the bone p late or
6、betwee n bone plate visible yellow small cavities, n amed lac un adaHSA實驗三、肌組織與神經(jīng)組織實驗二基本組織3報告主題:心肌主題屬性:指定標本號:12#材料:人心肌切片 染色:HE染色 教學要求:觀察人心肌的結(jié)構(gòu)特點,掌握其判斷依據(jù)閏盤In tercalated diskfl圖3心肌(人心肌切片,HE染色,40X 100)Figure 3 Cardiac muscle (human cardiac muscle smear HE stained 40 10X) dHtkD多數(shù)心肌纖維有一個細胞核,少數(shù)有雙核,核呈卵圓形,位
7、于細胞中央。心肌纖維的連接處稱閏盤,且染色深。心肌纖維呈明暗相間的周期性橫紋,但由于其肌原纖維粗 細不等,界限不明,因此橫紋不如平滑肌那么清楚。Edeu7。Most of the myocardial fibers have a nu cleus, a few have dual-core, and nu cleus is oval, located in the cen tral cell. The joint of the myocardial fibers called in tercalated disc, and dee p dye ing. The myocardial fiber
8、s are light and shade and p eriodic horiz on tal li nes, but as a result of its myofibril differe nt thick nesses of the unknown, so horiz on tal stri pes as smooth muscle so clear.utsnj?;窘M織4報告主題:多級神經(jīng)元主題屬性:指定標本號:9#材料:小牛脊髓及神經(jīng)節(jié)染色:HE染色教學要求:觀察脊髓前角多級神經(jīng)元的結(jié)構(gòu)特點,掌握其判斷依據(jù)*圖4多級神經(jīng)元(小牛脊髓切片,HE染色,40X 100)Figure 4
9、multipolar neuron (calf spinal cord smea, HE stained 40 1X) h8jBH多級神經(jīng)元細胞核有大、圓,亮的特點,胞體和突起中可見到許多紫藍色的不規(guī)則 的小斑塊,即尼氏體。突起從胞體發(fā)出,一個神經(jīng)元有多個樹突,一個軸突,因此軸突 很難切到,不容易看見。hfYyX。Multistage neurons nuclei have big, round, bright characteristics, cell and bumps in the visible to many p urple blue irregular small p atches
10、, n amely the Nissl body. Flash from the cell body out, a neuron have mult iple den drite, an axon, so it is difficult to cut the axon, which is not easy to seejeyG。實驗四消化管中空性器官1報告主題:空腸絨毛主題屬性:指定標本號:19#材料:貓空腸橫切片染色:HE染色教學要求:掌握空腸的結(jié)構(gòu)特征與判斷依據(jù),特別注意腸絨毛和腸腺的結(jié)構(gòu))r A紋狀緣Striated border單層柱狀上皮Sim pie colu mnar ep it
11、helium平滑肌Smooth muscle$fMMr T* -Smiz圖5空腸絨毛(貓空腸橫切片,HE染色,40 X 100)Figure5 jejunum villi (cat jejunum transverse section, HE stained 4 100) cudkK空腸絨毛上皮由吸收細胞、杯狀細胞和少量內(nèi)分泌細胞組成。吸收細胞數(shù)量最多, 呈高柱狀,核長橢圓型,位于基部,細胞游離有深染層,稱紋狀緣。絨毛中軸的固有層 為細密結(jié)締組織,其內(nèi)常可見縱行的平滑肌、豐富毛細血管、12條中央乳糜管(多數(shù)未切到)及大量淋巴細胞。Ew2bhThe ep ithelium of Jejunum
12、villi is comp osed of absor pti on, goblet cell and a small amount of en docri ne cells. The largest nu mber of absor ptive cells, a tall colu mnar, nu clear long elli ptic, is located in the base, cell free have hyp erchromatic layer, called striated border. Villi axis of the lam ina propria for fi
13、ne conn ective tissue, it ofte n visible Ion gitud inal done smooth muscle, rich cap illaries, 1 - 2 a cen tral lacteal (most not cut) and a large nu mber of lymp hocyteNdvm1實驗五消化管以外的其他中空性器官中空性器官2報告主題:中等動脈主題屬性:指定標本號:10#材料:狗中等動、靜脈橫切片染色:HE染色教學要求:掌握中等動脈的結(jié)構(gòu)特點y *Z/J *jLh HP。夕卜彈性膜 External elastic membran
14、e圖6中等動脈(狗中等動脈橫切片,HE染色,40 X 100)Figure 6 medium-sized artery (dog medium-sized artery and vei n tran sverse secti on smear , HE stained 40 100) wzBmX在高倍鏡下,可見內(nèi)彈性膜為一條呈波浪狀的折光性強的粉紅色亮帶,它是內(nèi)膜與 中膜的分界標志,中膜較厚,由數(shù)十層平滑肌纖維組成,因此中動脈也稱肌性動脈。外 膜由疏松結(jié)締組織構(gòu)成,多數(shù)中動脈的外膜和中膜之間有明顯的外彈性膜。T6tpt。At high magnification, internal elast
15、ic membrane is a wavy refraction sex strong pink light band, which is the border of tunica media and tunica adve ntitia .tunica media is con sist of doze ns of layer of the smooth muscle fibers, so the artery is also called the muscular artery. External elastic membra ne is comp osed of loose conn e
16、ctive tissue, the majority of the artery; there is obvious elastic membrane between tunica media and tunica adventitoJk。實驗六免疫系統(tǒng)實質(zhì)性器官1報告主題:淋巴結(jié)主題屬性:指定標本號:13#材料:狗淋巴結(jié)切片染色:HE染色教學要求:掌握淋巴結(jié)的結(jié)構(gòu)特征和判斷依據(jù)被膜Envelope淋巴小結(jié)Lymp hoid no dule圖7淋巴結(jié)(狗淋巴結(jié)切片,HE染色,40X100)Figure 7 Lymphoid node (Dog lymphoid smear , HE stain
17、ed , 40X 100) FWXVy 淋巴結(jié)多呈扁平豆形或者卵圓形,表面有薄層結(jié)締組織被膜,伸入實質(zhì)形成小梁, 小梁互相連接成網(wǎng)。皮質(zhì)位于被膜下,淺皮質(zhì)由淋巴小結(jié)和彌散淋巴組織構(gòu)成,副皮質(zhì) 為胸腺依賴區(qū),為較大片的彌散淋巴組織,皮質(zhì)淋巴竇位于被膜下方和小梁周圍.髓質(zhì)由髓索及其間的髓竇構(gòu)成。L882k。Lymph node is flat bea n sha ped or ovoid, the surface has a thi n layer of conn ective tissue cap sule, stretch into substa ntial form trabecular,
18、trabecular in terc onn ected into n etwork. Cortex is located in the envelope, shallow cortex by lymph no dule and dis persi on lymp hoid tissue structure; vice cortex for thymus depend on the area, for a large dispersion lymphoid tissue, cortex lymphatic sinus, in the envelope below and trabecular
19、meshwork. Medulla around by pulp line and the pulp sinus betwee n comp ositi ons.L7MhP實驗八腎與內(nèi)分泌系統(tǒng)實質(zhì)性器官2報告主題:下頜下腺 主題屬性:自選標本號:24#材料:人下頜下腺切片 染色:HE染色教學要求:掌握下頜下腺的三種腺泡和導管的結(jié)構(gòu)lURMa漿液性腺泡Serous acinus分泌管Secretary duCt半月(混合 性腺泡)Demil une*I/f*4 J八:宀黏液性腺泡Mucous acinus二 4 J T I - hr2 -* *-f4*.#24JlHE 染色,40X 100)圖8
20、下頜下腺(人下頜下腺切片,F(xiàn)igure 8 (Submandibular gland smearhuman submandibular gland HE stained 40 10X) 3ZZq3。下頜下腺為混合性腺,小葉內(nèi)可見很多圓形或不規(guī)則的腺泡,其中染紫紅色的是漿 液性腺泡,染色淺的是黏液性腺泡,混合性腺泡主要由黏液性腺細胞構(gòu)成,少量漿液性 腺細胞位于黏液性細胞之間或聚集在腺泡底部,也稱為半月。分泌管為單層柱狀上皮, 多且發(fā)達,閏管短而不明顯。QuUgioThe submandibular gland is mixed gland, the leaves can be seen many
21、 round or irregular acinus , in clud ing dye aborig ine is serous acinus, dye ing shallow is mucous acinus, mixed gla nd is mainly comp osed of mucous cells and a small amount of serous cells in the slime sex betwee n cells or gathered in gla nd bubble bottom, called demil une. Secretary duct for si
22、n gle colu mnar ep ithelium, and devel op ed, lea p tube short is not obviou匹eFu。實驗八腎與內(nèi)分泌系統(tǒng)實質(zhì)性器官3報告主題:腎單位主題屬性:指定標本號:29#材料:兔腎切片染色:HE染色教學要求:掌握腎單位各部分的結(jié)構(gòu)特點腎小囊Renal cap sule血管球Renal glomerulus9t3VV。圖9腎單位(兔腎切片,HE染色,40X 100)Figure 9 Nephron ( Rabbit kidney smea, HE stained , 40X00)4zswx腎單位由腎小體和與其相連的腎小管組成,腎
23、小體由血管球和腎小囊構(gòu)成,血管球 為腎小囊內(nèi)一團蟠曲的毛細血管,腎小囊是上皮性管道盲端膨大并凹陷的雙層囊。近曲 小管位于腎小體周圍,管徑大,管壁厚,管腔小而且不規(guī)則。遠曲小管與近曲小管混在 一起,管徑小,管壁薄,管腔較大而規(guī)則。dFrxo。Nephron is composed of renal corpuscle and renal tubule. Renal corpuscle by vascular ball and renal cap sule form, glomus for renal cap sule in a group of cap illary, renal cap sul
24、e is double cap sule. P roximal convo luted tubule in renal corp uscle around, pipe diameter, wall thick ness, the lume n is small and irregular. Distal convo luted tubule and p roximal convo luted tubule mixed together, pipe diameter is small, and the wall is thiswhgM實驗九生殖腺實質(zhì)性器官4報告主題:生精小管主題屬性:指定標本號
25、:41#材料:人睪丸與附睪切片染色:HE染色教學要求:了解睪丸與附睪的一般結(jié)構(gòu),掌握生精小管的結(jié)構(gòu)和間質(zhì)細胞的位置及 其結(jié)構(gòu)特點。IkYVSo圖10生精小管(人睪丸與附睪切片,HE染色,40X 100)Figure 10 Somn iferous tubule (huma n testis and ep ididymis , HE stai ned40X 100) uwxzs生精小管為高度彎曲的復層上皮性管道,外有薄層基膜,管壁由各級生精細胞和支 持細胞構(gòu)成。精原細胞附著在基膜上,胞體較小,核染色較深。初級精母細胞位于精原 細胞近腔側(cè),細胞體積大而圓,核亦大而圓,染色質(zhì)密集呈絨球狀。次級精母細
26、胞因存 在時間短不易找到,精子細胞位于近腔面,體積更小,核大而圓,染色質(zhì)細密。精子位 于腔面,頭部呈扁圓形,嵌入支持細胞的胞質(zhì)中,染色深,尾部游離于生精小管腔內(nèi)。U9yNfoSomn iferous tubules are curved stratified ep ithelium of pip eli ne, outside a thi n layer of basement membrane, wall by various raw sperm cell and support cells composition. Spermatophore attached on the basemen
27、t membrane, cell is lesser, nuclear staining deep. Primary spermatocyte located in nearly cavity side, cell volume is big and round, nuclear also large, round, chromatin concentrated in wool ball. Secondary spermatocyte there for short time is not easy to find, sperm cells in the nearly cavity surfa
28、ce, the volume is smaller, nuclear large, round, chromatin close. Sperm is located in the cavity surface, the head is oblateness, embedded support cell cytoplasm, dyeing deep, tail free born in somniferous tubule cavity.df9Cr 。自選主題實驗二、結(jié)締組織基本組織 5報告主題:嗜酸性粒細胞 主題屬性:自選 標本號: 7# 材料:人血涂片 染色: Wright 染色 教學要求:
29、掌握嗜酸性粒細胞紅細胞ErythrocyteSI圖11嗜酸性粒細胞(人血涂片,HE染色,40X100)Figure 11 Esoinophil(human blood smea, HE stained , 40X 100) isms嗜酸性粒細胞核多分為兩葉,胞質(zhì)內(nèi)充滿粗大、分布均勻、染成橘紅色、有折光性 的嗜酸性顆粒。其主要作用是能吞噬抗原抗體復合物,分解組胺,滅活白三烯,從 而減弱過敏反應,又參與對蠕蟲的免疫反應。lYtRj。Eosinophilic nuclear are mainiy divided into two leaves, cytoplasm filled with bulky
30、, distribution uniformity, dyed orange, refraction sex eosinophilic particles. Its main function is to devour an tige n an tibody comp lex, deco mp ositi on of histam ine, in activated leukotriene, thereby deadening allergic reaction, and the worm in the immune respon seFBvkM實驗上皮組織基本組織6報告主題:變移上皮主題屬性
31、:自選標本號:31#染色:HE染色材料:貓膀胱切片(空虛狀態(tài))教學要求:掌握膀胱空虛狀態(tài)下變移上皮的形態(tài)結(jié)構(gòu)蓋細胞Cover cells基膜Baseme nt membra ne2沁e小于V珞樂:圖12變移上皮 (貓膀胱切片,HE染色,40X100)Figure 12 Transitional epithelium (cat bladder smear HE stained , 40X 100) LotzF。 變移上皮由多層細胞構(gòu)成,基底面平整。細胞核常排成56層,其中,基底層細胞核密集,小且染色深;中間數(shù)層細胞多為多邊形或倒梨形,核圓;表層細胞大,分 界清晰,有防止尿液侵蝕的作用,稱為蓋細胞
32、。 cBkmyTransitional epithelium cells by multilayer structure, base with smooth surface. The nu cleus is ofte n in 5 6 layer, among them, the basal lam ina nu clei inten sive, small and dye ing dee p; In termediate layers of cells for po lyg on or fall p ear-sha ped, nu clear round; The surface cells
33、, boun dary clear, have p reve nt urine erosi on effect, called cover ceflfmQ 實驗四、消化管中空性器官3報告主題:食管主題屬性:自選標本號:18#染色:HE染色材料:狗食管和胃縱切片教學要求:掌握食管的結(jié)構(gòu)特點及判斷依據(jù)JGfYIP。圖13食管(狗食管和胃縱切片,HE染色,40X 100)Figure 13 Esop hagus (dog esop hagus and stomach Ion gitud inal sect ion, HE sta ined 40 X 100) t7cre。食管黏膜的上皮為復層扁平上皮
34、,固有層為細密結(jié)締組織,黏膜肌層由縱行平滑肌 束組成。黏膜下層的結(jié)締組織中存在許多黏液性的食管腺。肌層由內(nèi)環(huán)形和外縱行 兩層,人的食管上段為骨骼肌,下段為平滑肌。外膜為纖維膜。7YSWdEsophageal mucosa epithelial is stratified squamous epithelium, Muscularis mucosa is connective tissue, Iongitudinal beam of smooth muscle make up muscularis mucosa . Submucosa of conn ective tissue hasma ny
35、 esop hagealgla nds. musularis from inner ring and outer Iongitudinal two layer, human esophageal upper segment for skeletal muscle, lower segme nt for smooth muscle. Tunica adve ntitia is fiber membra nSLev。實驗七、消化腺與肺實質(zhì)性器官5肝自選報告主題: 主題屬性:標本號:26#染色:HE染色 材料:香豬肝切片教學要求:掌握肝的結(jié)構(gòu)特點及其判斷依據(jù)。zA5yC圖14肝(香豬肝切片,HE染色,40X100)Figure 14 liver (xiang pig liver smear , HE stained , 40X 100) uNvi5。肝是實質(zhì)性器官,外有結(jié)締組織被膜,伸入實質(zhì)將其分為許多小葉。小葉中央為中 央靜脈,以其為中心,肝板和肝血竇向周圍呈放射狀排列。肝板為肝細胞單層排列 的板狀結(jié)構(gòu),肝血竇為肝板之間的間隙。在相鄰小葉之間的結(jié)締組織為門管區(qū),其 內(nèi)可見小葉間動脈、靜脈及小葉間膽管。rMGaGLiver is substa ntive orga ns, the conn ective tissue cap su
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