常用緩沖溶液配制大全

1、常用緩沖溶液配制大全常用緩沖溶液的配制方法1 .甘氨酸-鹽酸緩沖液(0.05mol/L)X亳升0.2 mol/L曰氨酸+Y亳升0.2 mol/L HCI,再加水稀釋至200毫升PHXYPHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07,0.2 mol/L甘氨酸溶液含15.01克出。2 .鄰苯二甲酸-鹽酸緩沖液(0.05 mol/L)X毫升0.2 mol/L鄰苯二甲酸氫鉀+ 0.2 mol/L HCI,再加水稀程到20毫升pH(20)XYpH(20)XY2.254.0703.2

2、51.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022鄰苯二甲酸氫鉀分子量=204.23 . 0.2 mol/L鄰苯二甲酸氫溶液含40.85克小3 .磷酸氫二鈉由檬酸緩沖液pH0.2mol/LNa2Hp o4（亳升）O.lmol/L檸檬酸（亳升）pH0.2mol/LNa2Hp o4 （亳升）O.lmol/L檸檬酸（亳升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913

3、.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55NazHPO,分子量=14.98,0.2 mol/L 溶液為 28.40

4、克/升。Na2HPO42H2。分子量=178.05,0.2 mol/L 溶液含 35.01 克/升。C4H2。7+2。分子量=210.14,0.1 mol/L 溶液為 21.01 克小。4 .檸檬酸-氫氧化鈉-鹽酸緩沖液PH鈉離子濃度（mol/L）檸椽酸（克）C6H8O7*H2O氫氧化鈉（克）NaOH97%鹽酸（亳升）HCK 濃）最終體積（升）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610 使用時可以每

5、升中加入1克克酚，若最后pH值有變化，再用少量50%氫氧化鈉溶液或濃鹽酸調(diào)節(jié)，冰箱保存。5 .檸檬酸由檬酸鈉緩沖液（0.1 mol/L）pH0.1 mol/L檸檬酸0.1 mol/L檸檬酸鈉pH0.1 mol/L檸檬酸0.1 mol/L檸檬酸鈉（亳升）（亳升）（亳升）（亳升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.

6、610.39.76.61.418.64.89.210.8檸椽酸C6H8O7-H2O :分子量210.14,0.1 mol/L溶液為21.01克/升。檸檬酸鈉Na3 C6H5。72比0 :分子量294.12,0.1 mol/L溶液為29.41克/8升。6 .乙酸-乙酸鈉緩沖液（0.2 mol/L）pH（18）0.2 mol/LNa Ac（亳升）0.3 mol/LHAc（亳升）pH（18）0.2 mol/LNa Ac（S升）0.3 mol/LHAc德升）2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.65

7、7.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60Na2Ac-3H2O 分子量=136.09,0.2 mol/L 溶液為 27.22 克小。7 .磷酸鹽緩沖液(1)磷酸氫二鈉-磷酸二氫鈉緩沖液(0.2 )pH0.2 mol/LNa2HPO4僵升）0.3 mol/LNaH2PO4僵升）pH0.2 mol/LNa2HPO4（毫升）0.3 mol/LNaH2PO4（亳升）5.85.96.06.16.26.36.48.010.012.315.018.522.526.592.090.087.785.081.577.573.57.07.1

8、7.27.37.47.57.661.067.072.077.081.084.087.039.033.028.023.019.016.013.06.56.66.76.86.931.537.543.549.555.068.562.556.551.045.07.77.87.98.089.591.593.094.710.58.57.05.3Na2HPO4*2H2O 分子量=178.05,0.2 mol/L 溶液為 85.61 克/升。Na2HPO412H2。分子量=358.22,0.2 mol/L溶液為 71.64 克/升。Na2HPO4-2H2O 分子量=156.03,0.2 mol/L 溶液為 3

9、1.21 克/升。(2 )磷酸氫二鈉一磷酸二氫鉀緩沖液(1/15 mol/L)PHmol/L/15Na2HPO4像升）mol/L/15KH2PO4僵升）pHmol/L/15Na2HPO4（毫升）mol/L/15KH2PO4（亳升）4.920.109.907.17.003.005.290.509.5078.002.005.911.009.007.39.001.006.242.008.0089.500.506.473.007.007.79.750.256.644.006.0039.900.106.815.005.008.010.0006.986.004.0048.348.678.18Na2HPO4

10、2H2。分子量=178.05,1/15M 溶液為 1L876 數(shù)升。KH2P。4分子量=136.09,1/15M 溶液為 9.078 克陰。8 .磷酸二氫鉀-氫氧化鈉緩沖液（0.05M ）X毫升0.2M K2P。4 + 丫毫升0.2N NaOH加水稀釋至29亳升pH(20)X（毫升）Y（毫升）pH(20)X（亳升）丫（亳升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809 .巴比妥鈉-鹽酸緩沖液（18P ）pH0.04M巴比妥

11、鈉溶液0.2V鹽酸pH0.04M 巴比妥鈉溶液（亳升）0.2N鹽酸（毫升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥鈉鹽分子量=206.18;0.04M溶液為8.25克/升10 . Tris -鹽蹦沖液（0.05M , 25 ）50毫升0.1M三羥甲基氨基甲烷（Tris ）溶液與X亳升0.1N鹽酸混勻后，加 水稀釋至100毫升

12、。PHX（毫升）PHX（毫升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.908.0032.029.28.907.0三羥甲基氨基甲烷(Tris) H0CH2 CH20HCH0CH2 NH2分子量=121.14;0. 1M溶液為12.114克/升。Tris溶液可從空】中吸收二氧化碳，使用時注意將瓶蓋嚴。11.硼酸邯砂緩沖液（0.2 M硼酸根）pH0.05M 硼砂（亳升）0.2 M硼砂（

13、亳升）pH0.05M 硼砂（亳升）0.2 M硼酸（亳升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B4O7H2Q分子量=38L43;0.05M溶液（=0.2M硼酸根）含19.07泣。硼酸H2BO3,分子量=61.84,0.2M溶液為12.37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。11.硼酸-硼砂緩沖液（0.2M硼酸根）PH0.05M 硼0.2 M硼酸pH0.05M 硼0.2 M硼酸砂（亳升）（亳升）砂（亳升）（亳升）7.41.09.08.23.56.57.61.58.58.

14、44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B4O710H2Q分子量=38L43;0.05M溶液=0.2M硼酸根盒19.07克/升。硼酸H2BO3 ,分子量=61.84, 0.2M溶液為12.37克用。硼砂 易失去結(jié)晶水，必須在帶塞的瓶中保存。12 .甘氨酸-氫氧化鈉緩沖液（0.05M ）X亳升0.2M甘氨酸+Y毫升0.2NnaOH加水稀釋至200毫升PHXYpHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.

15、5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13 .硼砂-氫氧化鈉緩沖液（0.05M硼酸根）X亳升0.05M硼砂+Y亳升0.2NNaOH加水稀釋至200毫升PHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂Na2B4O710H2。,分子量=38L43;0.05M溶液為19.07克/升。14 .碳酸鈉-碳酸氫鈉緩沖液（0.1M ）Ca2+、Mg2+存在時不得使用pHOQMNa2c。3(毫0.1MN2HCO3(S2037升）升）9.168.77199.409.12289.519.40379.789.5046