常用緩沖溶液的配制

1、常用緩沖溶液的配制方法1.甘氨酸-鹽酸緩沖液(0.05mol/L)X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI，再加水稀釋至200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量 二二75.07，0.2 mol/L甘氨酸溶液含15.01克/升。2.鄰苯二甲酸-鹽酸緩沖液(0.05 mol/L)X毫升0.2 mol/L鄰苯二甲酸氫鉀+ 0.2 mol/L HCl，再加水稀釋到20毫升pH(20C)XYpH(20C)XY2.254.0703.251.4702

2、.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022鄰苯二甲酸氫鉀分子量二二204.23,0.2 mol/L鄰苯二甲酸氫溶液含40.85克/升3.磷酸氫二鈉-檸檬酸緩沖液pH0.2 mol/LNaHPO（毫升）0.1 mol/L檸檬酸（毫升）2.20.4010.602.41.2418.762.62.1817.822.83.1716.833.04.1115.893.24.9415.063.45.7014.303.66.4413.563.87.1012.904.07.7112.294.28.2811.724.48.8211.1

3、84.69.3510.654.89.8610.145.010.309.70pH0.2 mol/LNaHPO（毫升）0.1 mol/L檸檬酸（毫升）5.210.729.285.411.158.855.611.608.405.812.097.916.012.637.376.213.226.786.413.856.156.614.555.456.815.454.557.016.473.537.217.392.617.418.171.837.618.731.277.819.150.858.019.450.55NaHPO分子量=14.98,0.2 mol/L溶液為28.40克/升。NaHPO2H2O分子

4、量 二二178.05,0.2 mol/L溶液含35.01克/升。CBO7-HO分子量二二210.14,0.1 mol/L溶液為21.01克/升。4.檸檬酸-氫氧化鈉-鹽酸緩沖液pH鈉離子濃 度（mol/L）檸檬酸（克）GHO HO氫氧化鈉（克）NaOH97%鹽酸（毫升）HCI（濃）最終體積（升）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用時可以每升中加入1克克酚，若最后pH值有變化，再用少量50%

5、氫氧 化鈉溶液或濃鹽酸調(diào)節(jié)，冰箱保存。5.檸檬酸-檸檬酸鈉緩沖液（0.1 mol/L）pH0.1 mol/L檸檬酸（毫升）0.1 mol/L檸檬酸鈉（毫升）pH0.1 mol/L檸檬酸（毫升）0.1 mol/L檸檬酸鈉（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.21

6、0.8檸檬酸GfOHO分子量210.14,0.1 mol/L溶液為21.01克/升。檸檬酸鈉Na CeHsO2HO分子量294.12,0.1 mol/L溶液為29.41克/毫升。6.乙酸-乙酸鈉緩沖液（0.2 mol/L）pH(18C0.2 mol/L0.3 mol/LpH(18C0.2 mol/L0.3 mol/L)NaAcHAc)NaAcHAc（毫升）（毫升）（毫升）（毫升）2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.9

7、04.64.905.105.89.400.60NsbAc- 3H2O分子量二二136.09,0.2 mol/L溶液為27.22克/升。7.磷酸鹽緩沖液（1）磷酸氫二鈉-磷酸二氫鈉緩沖液（0.2）pH0.2 mol/L0.3 mol/LpH0.2 mol/L0.3 mol/LNazHPO（毫升）NafPO（毫升）5.88.092.05.910.090.06.012.387.76.115.085.06.218.581.56.322.577.56.426.573.56.531.568.56.637.562.56.743.556.56.849.551.06.955.045.0NaHPO（毫升）NafP

8、O（毫升）7.061.039.07.167.033.07.272.028.07.377.023.07.481.019.07.584.016.07.687.013.07.789.510.57.891.58.57.993.07.08.094.75.3NaHPQ2H2O分子量 二二178.05,0.2 mol/L溶液為85.61克/升。NaHPO12HO分子量 二二358.22,0.2 mol/L溶液為71.64克/升。NaHPO2H2O分子量 二二156.03,0.2 mol/L溶液為31.21克/升。pHmol/L/15Na2HPmol/L/15KH2pHmol/L/15Na2Hmol/L/15

9、KH2C4POPOPO（毫升）（毫升）（毫升）（毫升）4.920.109.907.7.003.00（2）磷酸氫二鈉-磷酸二氫鉀緩沖液（1/15 mol/L）5.290.509.50178.002.005.911.009.007.9.001.006.242.008.00389.500.506.473.007.007.9.750.256.644.006.00739.900.106.815.005.008.10.0006.986.004.00048.348.678.18NaHPQ2H2O分子量 二二178.05,1/15M溶液為11.876克/升。KHPQ分子量 二二136.09,1/15M溶液為9

10、.078克/升。8.磷酸二氫鉀-氫氧化鈉緩沖液（0.05M）X毫升0.2M K2PO + Y毫升0.2N NaOH加水稀釋至29毫升X (毫Y（毫升）pH(20CX (毫Y（毫升）pH(20C)升）)升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥鈉-鹽酸緩沖液（18C）pH0.04M巴比妥鈉溶液0.2V鹽酸pH0.04M巴比妥鈉溶液（毫升）0.2N鹽酸（毫升）6.810018.48.41005.217.01001

11、7.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥鈉鹽分子量=206.18;0.04M溶液為8.25克/升10.Tris-鹽酸緩沖液（0.05M,25C）50毫升0.1M三羥甲基氨基甲烷（Tris）溶液與X毫升0.1N鹽酸混勻后，加水稀釋至100毫升pHX（毫升）pHX（毫升）7.1045.78.1026.27.2044.78.2022.9硼砂NaBOH20,分子量=381.43;0.0

12、5M溶液（=0.2M硼酸根）含19.07克/升。7.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2二羥甲基氨基甲烷(Tris)HOCH2CH2OHCH0CH2 NH2分子量=121.14;0. 1M溶液為12.114克/升。Tris溶液可從空氣中吸收二氧化碳，使用時注意將瓶蓋嚴(yán)。11.硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼砂（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7

13、.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0甘氨酸分子量=75.07;0.2M溶液含15.01克/升。硼酸HBO,分子量=61.84,0.2M溶液為12.37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。11.硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼酸（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NQBO10HO

14、,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸HBO，分子量=61.84, 0.2M溶液為12.37克/升。硼砂易失去結(jié)晶水，必須在帶塞的瓶中保存。12.甘氨酸-氫氧化鈉緩沖液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀釋至200毫升pHXYpHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5NaCO10HO分子量=286.2;0.1M溶液為28.62克/升。13.硼砂-氫氧化鈉緩沖液（0.05M硼酸根）

15、X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀釋至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂NaBO-10HO,分子量=381.43;0.05M溶液為19.07克/升。14.碳酸鈉-碳酸氫鈉緩沖液（0.1M）Cd*、Mg+存在時不得使用pH0.1MNaCO（毫升）0.1M2HCO；毫升）20C37C9.169.409.519.789.9010.1410.2810.5310.838.779.129.409.509.729.9010.0810.2810.57123456789987654321NH

16、CO分子量=84.0;0.1M溶液為8.40克/升。PH7.67.47.27.0H0100010001000100mlNaCI8.58.58.58.5gNaHPO2.22.22.22.2gNaHPO0.10.20.30.4g15.“PBS緩沖液附錄四 常用緩沖溶液的配制（一）磷酸氫二鈉-檸檬酸緩沖液pH0.2 mol/LNa2HPO4(mL)0.1 mol/L檸檬酸(mL)2.20.4019.602.41.2418.762.62.1817.822.83.1716.833.04.1115.893.24.9415.063.45.7014.303.66.4413.563.87.1012.904.0乙

17、7112.29pH0.2 mol/LNa2HPO4(mL)0.1 mol/L檸檬酸(mL)5.210.729.285.411.158.855.611.608.405.812.097.916.012.637.376.213.226.786.413.856.156.614.555.456.815.454.557.016.473.534.23.2811.727.217.392.614.43.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HP0分子量=141.9

18、8;0.2 mol/L溶液為28.40 g/L。Na2HPO4 2H2O分子量=178.05;0.2 mol/L溶液為35.61 g/L。Na2HPO4 12H2O分子量=358.22;0.2 mol/L溶液為71.64 g/L。C6H8O7- H2O分子量=210.14;0.1 mol/L溶液為21.01 g/L。(二)檸檬酸-檸檬酸鈉緩沖液(0.1 mol/L)pH0.1 mol/L檸檬(mL)0.1 mol/L酸檸檬酸鈉(mL)pH0.1 mol/L檸檬酸(mL)0.1 mol/L檸檬酸鈉(mL)3.018.61.45.08.211.83.217.22.85.27.312.73.416.

19、04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8檸檬酸：C6H8O7 H2O分子量=210.14;0.1 mol/L溶液為21.01 g/L。檸檬酸鈉：Na3C6H5O72H2O分子量=294.12;0.1 mol/L溶液為29.41 g/L。(三)醋酸-醋酸鈉緩沖液(0.2 mol/L)pH0.2 mol/L0.2 mol/LpH0.2 mol/L0.2

20、mol/L(18C)NaAc (mL)HAc (mL)(18C)NaAc (mL)HAc (mL)3.60.759.354.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.86.400.60NaAc- 3H2O分子量=136.09;0.2 mol/L溶液為27.22 g/L。冰乙酸11.8 mL稀釋至1 L(需標(biāo)定)。(四)磷酸二氫鉀-氫氧化鈉緩沖液(0.05 mol/L)X毫升0.2 mol/L KH2PO4+Y毫升0.2

21、 mol/L NaOH加水稀釋至20毫升pH (20C)X (mL)Y (mL)pH (20C)X (mL)Y (mL)5.850.3727.052.9636.050.5707.253.5006.250.8607.43.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.680(五)磷酸氫二鈉-磷酸二氫鈉緩沖液(0.2 mol/L)pH0.2 mol/LNa2HPO4(mL)0.2 mol/LNaH2PO4(mL)pH0.2 mol/LNa2HPO4(mL)0.2 mol/LNaH2PO4(mL)5.88.092.07.061.039.0

22、5.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.051.08.094.75.36.955.045.0Na2HPO4 2H2O分子量=178.05;0.2 mol/L溶液為35.61 g/L。Na2HPO4 12H2O分子量=358.22;0.2

23、 mol/L溶液為71.64 g/L。NaH2PO4 H2O分子量=138.01;0.2 mol/L溶液為27.6 g/L。NaH2PO4 2H2O分子量=156.03;0.2 mol/L溶液為31.21 g/L。（六）巴比妥納-鹽酸緩沖液pH(18C)0.04 mol/L巴比妥鈉(mL)0.2 mol/LHCl (mL)PH(18C)0.04 mol/L巴比妥鈉(mL)0.2 mol/LHCl (mL)6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.2100

24、1.137.810011.479.41000.708.01009.399.61000.358.21007.21巴比妥鈉分子量=206.18;0.04 mol/L溶液為8.25 g/L。（七）Tris-HCI緩沖液（0.05 mol/L）50毫升0.1mol/L三羥甲基氨基甲烷（Tris）溶液與X毫升0.1mol/L鹽酸混勻并稀釋至100毫升。pH (25C)X (mL)pH (25C)X (mL)7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2Tris分子量=121.14;0.1 mol/L溶液為12.114 g/L