面向生物產(chǎn)業(yè)的高效菌種改造新方法及新設(shè)備的產(chǎn)學(xué)研合作研發(fā)-邢

1、 總結(jié) 高效微生物進(jìn)化育種技術(shù)是發(fā)酵生物技術(shù)發(fā)展的關(guān)鍵技術(shù)，對發(fā)酵產(chǎn) 業(yè)進(jìn)步具有重要意義。 ARTP 具有操作簡便、誘變速度快、突變多樣性大、能構(gòu)建大庫容突 變庫，是非GMO微生物進(jìn)化育種的有效方法，具有應(yīng)用推廣價值。 和系統(tǒng)生物學(xué)及代謝工程結(jié)合，ARTP是開發(fā)系統(tǒng)改造發(fā)酵微生物的 方法和技術(shù)的重要平臺。 H. Alper et al., Science 314, 1565. H. H. Wang et al., Nature 460, 894. Microalgae Other microbes ARTP mutation Mutant library Genomics Proteomics

2、 Metabolomics Evaluation Ideal mutants Non-GMO HTP screening Mutation mechanism Inverse ME Acknowledgements Collaborators: Prof. A. Kondo, Prof. S. Kajiwara, Prof. Z Liu Dr. Oda, Dr. Sang, Prof. J. Hu, Dr. Minsheng Liu and industry Research groups Department of Chemical Engineering Prof. Xin-Hui Xin

3、g, Assoc.Prof. Chong Zhang Dr. Li-Yan Wang, Dr. Hong-Xin Zhao, Dr. Zhongxuan Gou, Dr. Pei-Xia Jiang, Dr. Lihua Jin, Dr. Yuan Lu, Dr. Hao Wu PhD candidates: Zi-Liang Huang Mingyue Fang Xue Zhang MD student: Yunyi Chen Research Assistant: Mei Li, Qianqian Zhou Department of Engineering Physics Associate Prof. He-Ping Li Prof. Cheng-Yu Bao Dr. Nan Ge, Dr. Nie Graduates: Guo Li Sen Wang Pei-Si Le This work was supported by the JST CREST project of Japan and the Tsinghua University Initiative Scientific Research Program (2011THZ 01019. Good Design Precision Eng Car