Calcein-Blue - Dye Reagent - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemECalcein BlueCat. No.: HY-101887CAS No.: 54375-47-2分式: CHNO分量: 321.28作靶點(diǎn): Others作通路: Others儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 6 mg/mL (18.68 mM; Need ultrasonic and warming)Mass

2、 Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 3.1125 mL 15.5627 mL 31.1255 mL5 mM 0.6225 mL 3.1125 mL 6.2251 mL10 mM 0.3113 mL 1.5563 mL 3.1125 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Calcein Blue于標(biāo)記活細(xì)胞的短效藍(lán)熒光染料。體外研究Calcein blue AM is only weakly fluorescent (excitation/

3、emission maxima 322/435 nm). Upon cleavage of theAM esters by intracellular esterases, this tracer becomes relatively polar and is retained by cells for severalhours. In addition, its fluorescence intensity increases and its fluorescence spectra shift to longerwavelengths, with excitation/emission m

4、axima of 360/449 nm. The fluorescence of Calcein Blue is known tobe quenched in the presence of iron(III); if a stronger chelator removes this ion from the fluorophore, the1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEfluorescence of the fluorophore is regained 1. A novel fluorimetric assay for d

5、opamine using calcein blue(CB) complexed with Fe2 ion as a chemical sensor is described. The fluorescence arising from calcein blueof the calcein blueFe2 complex is quenched by the Fe2 ion. When dopamine is added to a solution of thecalcein blueFe2 complex, a dopamineFe2 complex is formed as the res

6、ult of a ligand exchange reactionbetween calcein blue and dopamine which permits the fluorescence from calcein blue to be recovered. Thefluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to beproportional to the concentration of the dopamine added to

7、 the calcein blueFe2 complex solution, whichpermits dopamine to be quantitatively determined 2.PROTOCOLCell Assay The recommended final working concentrations is usually between 1 and 10 M to minimize potentialartifacts. Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processin

8、g of the dyes. A stockconcentration of 5 mM 4-methylumbelliferone-8- methyliminodiacetic acid, commonly known as calcein blueis prepared in 0.1 M potassium hydroxide, and the pH is neutralized using 0.1 N hydrochloric acid. Thecalcein blue stock prepared is diluted to 200 M (working stock) in DPBS a

9、nd stored for further use. Aconcentration of 10 M calcein blue is prepared from the working stock by diluting in DPBS. From this, 200 L is added to a 96-well plate and the excitation/emission (Ex./Em.) maximum is determined using the micro-plate reader 1.MCE has not independently confirmed the accur

10、acy of these methods. They are for reference only.REFERENCES1. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, withpotential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49.2. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012May 30;94:36-43.McePdfHeightCaution: Product has not been ful