實驗室常用數(shù)據(jù)

標準緩沖液pH值與溫度對照表溫度°C四草酸氫鉀0.05M鄰苯二甲酸氫鉀0.05M混合磷酸鹽0.025M硼砂0.01M51.674.006.959.39101.674.006.029.33151.674.006.909.28201.684.006.889.23251.684.006.869.18301.684.016.859.14351.694.026.849.11常用蛋白質(zhì)分子量標準參照物高分子量標準參照中分子量標準參物低分子量標準參照蛋白質(zhì)Mr蛋白質(zhì)Mr蛋白質(zhì)Mr肌球蛋白212000磷酸化酶B97400碳酸酎酶31000P一半乳糖苷酶116000牛血清清蛋白66200大豆胰蛋白酶制劑21500磷酸化酶B97400谷氨酸脫氫酶55000馬心肌球蛋白16900牛血清清蛋白66200卵清蛋白42700溶菌酶14400過氧化氫酶57000醛縮酶40000肌球蛋白（F1）8100醛縮酶40000碳酸酎酶大豆胰蛋白酶抑制劑溶菌酶310002150014400肌球蛋白（F2）肌球蛋白（F3）62002500層析法常用數(shù)據(jù)表及性質(zhì)（一）離子交換纖維素目前常用的離子交換纖維素列于下表DEAE-形狀長度交換當量（毫克當量/克）蛋白吸附容量（毫克/克）床體積（毫升/克）纖維素（微米）胰島素(pH8.5)牛血清清蛋白（pH8.5）pH6.0pH7.5DE-22改良纖維性12~4001.0±0.17504507.77.7DE-23*18~4001.0±0.17504508.39.1DE-32同上（除細24~631.0±0.18506606.06.3DE-52CM-纖維素粒）微粒性（十24~631.0±0.18506607S-y球蛋6.06.3粉）溶菌酶pH5.0pH7.5同上（溶脹）(pH5.0)白(pH5.0)CM-2212~4000.6+0.066001507.77.7

CM-2318~4000.6+0.066001509.19.1CM-3224~631.0±0.11,2604006.86.7CM-52改良纖維性同上（除細粒）微粒性（十粉）同上（溶脹）24~631.0+0.11,2604006.86.7*：英國Whatman廠的型號，原來有舊型號如DE-1,為長纖維性，長度1000微米。還有DE-11,纖維性，50?250微米，對牛血清清蛋白的吸附容量僅為130毫克/克。（二）聚丙烯酰胺凝膠的技術(shù)數(shù)據(jù)型號排阻的下限（Mr）分級分離的范圍（Mr）膨脹后的床體積（mL/g十凝膠）膨脹所需最少時間（室溫，小時）Bio-gel-P-21600200~20003.82~4Bio-gel-P-43600500~40005.82~4Bio-gel-P-646001000~50008.82~4Bio-gel-P-10100005000~1700012.42~4Bio-gel-P-303000020000~5000014.910~12Bio-gel-P-606000030000~7000019.010~12Bio-gel-P-10010000040000~10000019.024Bio-gel-P-15015000050000~15000024.024Bio-gel-P-20020000080000~30000034.048Bio-gel-P-300300000100000~40000040.048注：上述各種型號的凝膠都是親水性的多孔顆粒，在水和緩沖溶液中很容易膨脹，生產(chǎn)廠為Bio-RadLaboratories,Rich-mond,California,U.S.A.（三）瓊脂糖凝膠的技術(shù)數(shù)據(jù)（瓊脂糖是瓊脂內(nèi)非離子型的組分，它在0~4°C，pH4~9范圍內(nèi)是穩(wěn)定的。）名稱、型號凝膠內(nèi)瓊脂糖百分含量（W/W）排阻的下限（Mr）分級分離的范圍（Mr）生產(chǎn)廠商Sagavac10102.5X1051X10廣2.5X105SeravacSagavac887X1052.5X10廣7X105Laboratories,Sagavac662X1065X10廣2X106Maidenhead,Sagavac4415X1062X105~15X106EnglandSagavac22150X1065X105~15X107Bio-GelA-0.5M100.5X105<1X10廣2.5X106Bio-RadBio-GelA-1.5M81.5X106<1X10廣1.5X106Laboratories,Bio-GelA-5M65X1061X10廣5X106California,U.S.A.Bio-GelA-15M415X1054X10廣15X106Bio-GelA-50MBio-GelA-150M2150X106150X1061X105~50X1061X106~150X106（四）凝膠過濾層析介質(zhì)的技術(shù)數(shù)據(jù)凝膠過濾介質(zhì)名稱分離范圍顆粒大?。ā╩）特性/應用pH穩(wěn)定性工作耐壓(Mpa)最快流速（cm/h）Superdex30<1000024~44肽類、寡糖、小蛋3~120.3100Superdex753000~7000024~44白等3~120.3100300024~44重組蛋白、細胞色3~120.3100Superdex20010000~60000020~40素3~120.430Superose65000~5X10620~40單抗、大蛋白3~120.730Superose121000~30000025~75蛋白、肽類、多糖、3~110.220~39Sephacryl1000~100000核酸S-100HR25~75蛋白、肽類、寡糖、3~110.220~39Sephacryl5000~250000多糖S-200HR25~75肽類、小蛋白3~110.220~39Sephacryl10000~1.5X106S-300HR25~75蛋白，如清蛋白3~110.220~39Sephacryl20000~8X106S-400HR平均90蛋白、抗體2~120.1300Sepharose610000~4X106FastFlow平均90多糖、具延伸結(jié)構(gòu)2~120.1250Sepharose460000~20X106的大分FastFlow60~200子如蛋白多糖、脂4~90.00410Sepharose2B70000~40X106質(zhì)體巨大分子4~90.00811.5Sepharose4B60000~20X10645~1650.0214Sepharose6B10000~4X10645~165巨大分子如重組4~90.00515Sepharose70000~40X10660~200乙型肝CL-2B炎表面抗原3~130.01226Sepharose60000~20X10545~165蛋白、大分子復合3~130.0230CL-4B10000~4X10645~165物、病毒、不對稱Sepharose分子如核酸和多CL-6B糖（蛋白多糖）蛋白、多糖蛋白、多糖蛋白、大分子復合物、病毒、不對稱分子如核酸和多糖（蛋白多糖）蛋白、多糖蛋白、多糖凝膠過濾介質(zhì)名稱分離范圍顆粒大?。∕m）特性/應用pH穩(wěn)定性工作干凝膠溶脹體積（mL/g）溶脹最少平衡時間（h）最快流速（cm/h）室溫沸水SephadexG-10<700十粉40~1202~132~3312~5SephadexG-15<1500十粉2~132.5~3.5312~5SephadexG-251000~500040~120脫鹽及交換2~134~6622~5Coarse十粉緩沖液用SephadexG-25Medium1000~5000100~300脫鹽及交換緩沖液用2~134~6622~5SephadexG-25Fine1000~5000十粉脫鹽及交換2~134~6622~5SephadexG-2550~150緩沖液用Superfine1000~5000脫鹽及交換2~134~6622~5SephadexG-50十粉緩沖液用Coarse1500~3000020~80小分子蛋白2~109~11622~5SephadexG-50Medium十粉質(zhì)分離1500~3000010~40小分子蛋白2~109~11622~5SephadexG-50Fine質(zhì)分離SephadexG-501500~30000十粉小分子蛋白2~109~11622~5Superfine100~300質(zhì)分離SephadexG-751500~30000十粉小分子蛋白2~109~11622~550~150質(zhì)分離SephadexG-75Superfine3000~80000十粉中等蛋白質(zhì)分離2~1012~1524372SephadexG-1003000~7000020~80中等蛋白質(zhì)2~1012~1524316SephadexG-100Superfine4000~1.5X105十粉分離2~1015~2048547

SephadexG-150SephadexG-150SuperfineSephadexG-200SephadexG-200Superfine4000~1X1055000~3X1055000~1.5X1055000~6X1055000~2.5X10510~40十粉40~120十粉10~40十粉40~120十粉10~40十粉40~120十粉10~40十粉40~120十粉10~40中等蛋白質(zhì)分離中等蛋白質(zhì)分離稍大蛋白質(zhì)分離稍大蛋白質(zhì)分離較大蛋白質(zhì)分離較大蛋白質(zhì)分離2~102~102~102~102~1015~2020~3018~2230~4020~2548727272725555511215.6112.8SephadexLH20(嗜脂性)100~4000十粉25~106特別為使用有機溶劑而設計。適合分離脂類、膽固醇、脂肪酸、激素、維生素及其他小生物分子。此分離范圍指以酒精為溶劑的分離(五)離子交換層析介質(zhì)的技術(shù)數(shù)據(jù)離子交換介質(zhì)名稱最高載量顆粒大小(Mm)特性/應用pH穩(wěn)定性工作耐壓(MPa)最快流速(cm/h)SOURCE15Q25mg蛋白152?1241800SOURCE15S25mg蛋白152?1241800QSepharoseH.P.70mgBSA24?442?120.3150

SPSepharoseH.P.55mg核糖核酸酶24?443?120.3150QSepharoseF.F.120mgHSA45?1652?120.2400SPSepharoseF.F.75mgBSA45?1654?130.2400DEAESepharoseF.F.110mgHSA45?1652?90.2300CMSepharoseF.F.50mg核糖核酸酶100?3006?130.2300QSepharoseBigBeads100?3002?120.31200?1800SPSepharoseBigBeads60mgBSA十粉40?1204?120.31200?1800離子交換介質(zhì)名稱最高載量顆粒大小(Mm)特性/應用pH穩(wěn)定性工作耐壓(MPa)最快流速(cm/h)QAESephadexA-251.2mg甲狀腺球蛋白80mgHSA十粉40?120純化低分子量蛋白、多肽、核苷以及巨大分子(Mr>200000),在工業(yè)傳統(tǒng)應用上具有重要作用2?100.11475QAESephadex1.2mg甲狀腺球蛋白十粉40?120批量生產(chǎn)和預處理用，分離中等大小的生物分子2?110.0145

A-5080mgHSA(30?200000)SPSephadexC-251.1mgIgG70mg牛羰合血紅蛋白230mg核糖核酸酶十粉40?120純化低分子量蛋白、多肽、核苷以及巨大分子(Mr>200000),在工業(yè)傳統(tǒng)應用上具有重要作用2?100.13475SPSephadexC-508mgIgG110mg牛羰合血紅蛋白十粉40?120批量生產(chǎn)和預處理用，分離中等大小的生物分子(30?200000)2?100.0145DEAESephadexA-251mg甲狀腺球蛋白30mgHAS140mga-乳清蛋白十粉40?120純化低分子量蛋白、多肽、核苷以及巨大分子(Mr>200000),在工業(yè)傳統(tǒng)應用上具有重要作用2?90.11475DEAESephadexA-502mg甲狀腺球蛋白110mgHSA十粉40?120批量生產(chǎn)和預處理用，分離中等大小的生物分子(Mr>200000),在工作傳統(tǒng)應用上具有重要作用2?90.1145CMSephadexC-251.6mgIgG70mg牛羰合血紅蛋白190mg核糖核酸酶十粉40?120純化低分子量蛋白、多肽、核苷以及巨大分子(Mr>200000),在工業(yè)傳統(tǒng)應用上具有重要作用6?130.13475CMSephadexC-507mgIgG140mg牛羰合血紅蛋白120mg核糖核酸酶十粉40?120批量生產(chǎn)和預處理用，分離中等大小的生物分子(30?200000)6?100.0145常用緩沖溶液的配制方法1.甘氨酸-鹽酸緩沖液(0.05mol/L)X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI，再加水稀釋至200毫升

2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量二75.07,0.2mol/L甘氨酸溶液含15.01克/升。2.鄰苯二甲酸-鹽酸緩沖液（0.05mol/L）X毫升0.2mol/L鄰苯二甲酸氫鉀+0.2mol/LHCl，再加水稀釋到20毫升pH(20r)XYpH(20°C)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022鄰苯二甲酸氫鉀分子量二204.23，0.2mol/L鄰苯二甲酸氫溶液含40.85克/升3.磷酸氫二鈉-檸檬酸緩沖液pH0.2mol/LNaHPO(毫升)0.1mol/L檸檬酸（毫升）pH0.2mol/LNaHPO(毫升)0.1mol/L檸檬酸（毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55NaHPO分子量=14.98,0.2mol/L溶液為28.40克/升。Na2HPo4-2HO分子量=178.05,0.2mol/L溶液含35.01克/升。cHo.4HO2分子量二210.14,0.1mol/L溶液為21.01克/升。42724.檸檬酸-氫氧化鈉-鹽酸緩沖液pH鈉離子濃度（mol/L）檸檬酸（克）CHO.HO6872氫氧化鈉（克）NaOH97%鹽酸（毫升）HCl（濃）最終體積（升）①

2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610使用時可以每升中加入1克克酚，若最后pH值有變化，再用少量50%氫氧化鈉溶液或濃鹽酸調(diào)節(jié)，冰箱保存。②5.檸檬酸-檸檬酸鈉緩沖液（0?1mol/L）pH0.1mol/L檸檬酸（毫升）0.1mol/L檸檬酸鈉（毫升）pH0.1mol/L檸檬酸（毫升）0.1mol/L檸檬酸鈉（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8檸檬酸C6H8O7.檸檬酸鈉Na3HO：分子量2qHQ.2H2O：210.14,0.1mol/L溶液為21.01克/升。分子量294.12,0.1mol/L溶液為29.41克/毫升。6.乙酸-乙酸鈉緩沖液（0.2mol/L）pH（18°C）0.2mol/LNaAc（毫升）0.3mol/LHAc（毫升）pH（18C）0.2mol/LNaAc（毫升）0.3mol/LHAc（毫升）2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60NaAc.3HO分子量=136.09,0.2mol/L溶液為27.22克/升。227.磷酸鹽緩沖液（1）磷酸氫二鈉-磷酸二氫鈉緩沖液（0.2）pH0.2mol/LNaHPO0.3mol/LNaHPO24pH0.2mol/LNaHPO0.3mol/LNaHPO24

（毫升）（毫升）（毫升）（毫升）5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0NaHPO.2HO分子量二178.05,0.2mol/L溶液為85.61克/升。Na：HPO；2H：O分子補充日期：2004-12-1401:03:56NaHPO.2HO分子量二358.22,0.2mol/L溶液為71.64克/升。Na：HPO4.2H：O分子量二156.03,0.2mol/L溶液為31.21克/升。（2）磷酸氫二鈉-磷酸二氫鉀緩沖液（1/15mol/L）pHmol/L/15NaHPOmol/L//15KHPOpHmol/L//15NaHPOmol/L//15KHPO（毫升）24（毫升）24（毫升）24（毫升）244.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00NaHPO.2HO分子量二178.05,1/15M溶液為11.876克/升。24_2、、，rKHPO分子量=136.09,1/15M溶液為9.078克/升。8.2磷4酸二氫鉀-氫氧化鈉緩沖液（0.05M）X毫升0.2MKPO+Y毫升0.2NNaOH加水稀釋至29毫升pH(20C)24X（毫升）Y（毫升）pH(20C)X（毫升）Y（毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥鈉-鹽酸緩沖液（18°C）

pH0.04M巴比妥鈉溶液0.2V鹽酸pH0.04M巴比妥鈉溶液（毫升）0.2N鹽酸（毫升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥鈉鹽分子量=206.18;0.04M溶液為8.25克/升10.Tris-鹽酸緩沖液（0?05M,25°C）50毫升0.1M三羥甲基氨基甲烷（Tris）溶液與X毫升0.1N鹽酸混勻后，加水稀釋至100毫升。pHX（毫升）pHX（毫升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羥甲基氨基甲烷（Tris）HOCH2CH2OHCHOCH2NH2分子量=121.14;0.1M溶液為12.114克/升。Tris溶液可從空氣中吸收二氧化碳，使用時注意將瓶蓋嚴。11.硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼砂（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBO.HO,分子量二381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。,247,_2一、、-.硼酸HBO,分子量二61.84,0.2M溶液為12.37克/升。硼砂房失去結(jié)品水，必須在帶塞的瓶中保存。

11?硼酸-硼砂緩沖液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼酸（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂NaBO.10HO,分子量二381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。,247,_、、-.硼酸HBO，分子量=61.84,0.2M溶液為12.37克/升。硼砂23易失去結(jié)品水，必須在帶塞的瓶中保存。12.甘氨酸-氫氧化鈉緩沖液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀釋至200毫升pHXYpHXY8.6504.09.65022.48.8506.09.85027.29.0508.810.05032.09.25012.010.45038.69.45016.810.65045.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13.硼砂-氫氧化鈉緩沖液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀釋至200毫升pHXYpHXY9.3506.09.85034.09.45011.010.05043.09.65023.010.15046.0硼砂NaBO.10HO,分子量二381.43;0.05M溶液為19.07克/升。247214.碳酸鈉-碳酸氫鈉緩沖液（0.1M）Ca2+、Mg2+存在時不得使用pH0.1MNa2CO3（毫升）0.1MN2HCO3（毫升）20°C37C9.168.77199.409.12289.519.40379.789.50469.909.725510.149.906410.2810.087310.5310.288210.8310.5791Na2CO2.10H2O分子量二286.2;0.1M溶液為28.62克/升。

N2HCO3分子量=84.0;0.1M溶液為8.40克/升。15.“PBS”緩沖液PH7.67.47.27.0HO100010001000100ml2NaCl8.58.58.58.5gNaHPO2.22.22.22.2g24NaHPO240.10.20.30.4g實驗室中常用酸堿的比重和濃度名稱分子式分子量比重面分濃度%（W/W）當量濃度（粗略）N配1升1N溶液所需毫升數(shù)鹽酸HCl36.471.1937.212.0841.1835.411.81.1020.06.0硫酸H2SO498.091.8495.636.0281.1824.86.0硝酸HNO263.021.4270.9816.0631.4065.314.51.2032.366.1冰乙酸CHCOOH360.051.0599.517.459乙酸CHCOOH398.06366.0磷酸H2PO435.051.7185.015,30,45（依反應而定）67（以15N計）氨水NHOH40.9015670.90427.014.3700.9125.013.40.9610.05.6氫氧化鈉溶液NaOH40.01.550.01953硫酸銨飽和度的常用表1.調(diào)整硫酸銨溶液飽和度計算表（25°C）硫整硫酸鉉終濃度,％飽和度10202530333540455055606570758090100每1升溶液加固體硫酸鉉的克數(shù)①硫酸鉉0561141441761962092432773133513904304725165616627671057861181371501832162512883263654064494945926942029597891123155190225262300340382424520619初濃度，%飽和度2530496193125158193230267307348390485583301930629412716219823527331435644954633124374107142177214252292333426522353163941291642002381783194115064031639713216820524528537546945326599134171210250339431503366101137176214302392553367103141179264353603469105143227314653470107190275703572153237753611519880771579079①在25C下，硫酸銨溶液由初濃度調(diào)到終濃度時，每升溶液所加固體硫酸銨的克數(shù)。2.調(diào)整硫酸銨溶液飽和度計算表（0C）①在0°C下，硫酸銨溶液由初濃度調(diào)到終濃度時，每100毫升溶液所加固體硫酸銨的克數(shù)。蛋白質(zhì)分子量肌球蛋白[myosin]220,000甲狀腺球蛋白[thyroglobulin]165,000常見蛋白質(zhì)分子量參考值（單位:dalton）P-半乳糖苷酶[。-galactosidase]130,000副肌球蛋白［paramyosin］100,000磷酸化酶a[phosphorylasea]94,000血清白蛋白[serumalbumin]68,000L-氨基酸氧化酶[L-aminoacidoxidase]63,000地氧化氫酶［catalase］60,000丙酮酸激活酶[pyruvatekinase]57,000谷氨酸脫氫酶[glutamatedehydrogenase]53,000亮氨酸氨肽酶[glutamaedehydrogenase]53,000Y-球蛋白，H鏈[y-globulin,Hchain]50,000延胡索酸酶（反丁烯二酸酶）［fumarase］49,000卵白蛋白[ovalbumin]43,000醇脫氫酶(肝)[alcoholdehydrogenase(liver)]41,000烯醇酶[enolase]41,000醛縮酶[aldolase]40,000肌酸激酶[creatinekinase]40,000胃蛋白酶原［pepsinogen］40,000D-氨基酸氧化酶[D-aminoacidoxidase]37,000醇脫氫酶(酵母)[alcoholdehydrogenase(yeast)]37,000甘油醛磷酸脫氫酶[dlyceraldehydephosphatedehydrogenase]36,000原肌球蛋白［tropomyosin］36,000乳酸脫氫酶[lactatedehydrgenase]36,000胃蛋白酶［pepsin］35,000轉(zhuǎn)磷酸核糖基酶[phosphoribosyltransferase]35,000天冬氨酸氨甲酰轉(zhuǎn)移酶，C鏈[aspertatetranscarbamylase,Cchain]34,000羧肽酶A[carboxypeptidaseA]34,000碳酸酎酶[carbonicanhydrase]29,000枯草桿菌蛋白酶［subtilisin］27,600y-球蛋白，L鏈y-blobulin,Lchain[]23,500糜蛋白酶原（胰凝乳蛋白酶原）［chymotrypsinogen25,700胰蛋白酶［trypsin］23,300木瓜蛋白酶（羧甲基）［papain（carboxymethyl）］23,000P-乳球蛋白[。-lactoglobulin]18,400煙草花葉病毒外殼蛋白（TWV外殼蛋白）［TWVcoatprotein17,500肌紅蛋白[myoglobin]17,200天門冬氨酸氨甲酰轉(zhuǎn)移酶，R鏈[aspartatetranscarbamylase,Rchain]17,000血紅蛋白[h(a)emoglobin]15,500Qp外殼蛋白[Qpcoatprotein]15,000溶菌酶[lysozyme]14,300R外殼蛋白[Rcoatprotein]13,750核糖核酸酶[ribonuclease或RNase]13,700細胞色素C[cytochromeC]11,700糜蛋白酶（胰凝乳蛋白酶）［chymotrypsin］11,000或13,000常見蛋白質(zhì)等電點參考值（單位：pH）蛋白質(zhì)等電點鮭精蛋白[salmine]鯡精蛋白[clupeine]鱘精蛋白[sturline]胸腺組蛋白[thymohistone]珠蛋白(人)[globin(human)]卵白蛋白[ovalbuin]伴清蛋白[conalbumin]血清白蛋白[serumalbumin]肌清蛋白[myoalbumin]肌漿蛋白[myogenA]P-乳球蛋白[。-lactoglobulin]卵黃蛋白[livetin]—球蛋白(人)[丫-globulin(human)]—球蛋白(人)[丫-globulin(human)]肌球蛋白A[myosinA]原肌球蛋白[myosinA]鐵傳遞蛋白[siderophilin]胎球蛋白[fetuin]血纖蛋白原[fibrinogen]a-眼品體蛋白[a-crystallin]p-眼品體蛋白[。-crystallin]花生球蛋白[arachin]伴花生球蛋白[conarrachin]角蛋白類[keratins]還原角蛋白[keratein]膠原蛋白[collagen]魚膠[ichthyocol]白明膠[gelatin]a-酷蛋白[a-casein]p-酷蛋白[p-casein]-酷蛋白[Y-casein]a-卵清粘蛋白[a-ovomucoid]a-粘蛋白[a-mucoprotein]卵黃類粘蛋白[vitellomucoid]尿促性腺激素[urinarygonadotropin]溶菌酶[lysozyme]肌紅蛋白[myoglobin]血紅蛋白(人)[hemoglobin(human)]血紅蛋白(雞)[hemoglobin(hen)]血紅蛋白(馬)[hemoglobin(horse)]血藍蛋白[hemerythrin]蚯蚓血紅蛋白[chlorocruorin]12.112.111.7110.87.54.71;4.596.8;7.14.93.56.35.35.05.8;6.67.3;8.25.55.15.93.55.84.86.05.13.95.04.76.85.25.04.0-4.14.56.03.83-4.412.75.53.311.0-11.26.997.077.236.926.45.6

血綠蛋白[chlorocruorin]4.3-4.5無脊椎血紅蛋白[erythrocruorins]4.6-6.2細胞色素C[cytochr