細環(huán)病毒從實驗室到臨床（課堂PPT）

細環(huán)病毒：從實驗室到臨床Torquetenovirus:thehumanviromefrombenchtobedside文獻匯報D.Focosietal.ClinicalMicrobiologyandInfection22(2016)589-5931TTV:Transfusiontransmittedvirus

orTorquetenovirusFromtorquesandtenuis,Latinfor‘necklace’and‘thin,’respectively輸血后肝炎（肝中病毒載量是血中的10-100倍，并且與血清ALT升高相關(guān)）【現(xiàn)已否定該結(jié)論】Humanviromeatleast29majorspecies細環(huán)病毒：首個被發(fā)現(xiàn)的單鏈環(huán)狀DNA病毒2TTVprevalence>95%inmostcasesindependentofage,socioeconomic

standingandhealth

conditions來源：胎盤、呼吸道、輸血分布：T淋巴細胞現(xiàn)有臨床應(yīng)用：TTVasaMarkerofAnthropicPollutionTTVasaMarkerofImmuneStatusOrphanvirus~孤兒病毒（非病原性病毒）3檢測水源是否被污染：現(xiàn)有方法為檢測細菌，但是很容易出現(xiàn)假陽性某些情況下即使細菌檢測為陰性也不能排除被污染檢測病毒更準確TTV分布廣泛：醫(yī)院空氣、物件表面均可存在TTVasaMarkerofAnthropicPollutionTTVasaMarkerofImmuneStatus免疫功能完整性：theabilitytomountaprotectiveimmuneresponseagainstanantigenicstimulation.現(xiàn)有檢測：抗原特異性，抗原非特異性；檢測繁瑣，檢測方法缺乏標準化TTV與CD8+57+T淋巴細胞比例成負相關(guān)對抗病毒藥不敏感4TTVasaMarkerofImmuneStatusexcessive(toomanyopportunisticinfectionsandcancers)inadequate(toomanyepisodesofcellularacuterejection)Maintenanceimmunosuppression5WhyIsTTVNotYetPartofaDiagnosticToolKit?現(xiàn)有研究不足無法培養(yǎng)無抗體檢測試劑無動物模型PCR方法缺乏標準化（引物設(shè)計）特異性引物非特異性引物6TakehomemessagesTTVisatypeofOrphanvirus,prevalence>95%inmostcasesTTVreplicatesmainlyinTlymphocytesInsensitivetocurrentantiviraldrugs

TTVasaMarkerofImmun