內(nèi)容課件教案

10.CytoskeletonandCellMotilityOrganizationAndFunctionsOftheMicrotubule(微管Microfilament(微絲Intermediatefilament(中間纖維OrganizationanddistributionofcytoskeletonCytoskeleton:filamentousproteinsdistributeinthenucleusandbeneaththeplasma一、Typesmicrotubule(微管)(微絲)intermediatefilament(中間纖維MicrotubuledistributesmainlyoutsidetheMicrofilamentdistributesmainlyinsidetheplasmaandnuclearIntermediatefilamentdistributesinthewholeCharacters:1.DistributedispersedlyandconnectwitheachothertoformaninteractivenetworkSensitivetophysiologicalRestrictedbyfactorsinsideandoutsidethecellMvidestructuralsupportthatcandeterminetheshapeofthecell2.directthemovementofmaterialsandorganelleswithincells3.movecellsfromoneplaceto4.anessentialcomponentofthecell’sdivisionmachinery5.transport structureandshape:a.hollow,tubularbconsistsof13protofilaments(原纖維c.outerdiameter—25nminnerdiameter---15nm.Tubulin(微管蛋白 eachprotofilamentconsistofα-tubulinandβ-tubulinglobularsubunitβγ-helpmicrotubuleform(幫助微管成核shaft:桿（本體 TypesofSinglet(單管)beneaththeplasmanetwork,Doublet(二聯(lián)管)consistofAandBtheshaftofcilia(纖毛)andflagella(鞭毛Triplet(三聯(lián)管)consistofA,BandCtubules;basalbodyofciliaandflagella,centrioleMicrotubule-associated(MAP,微管結(jié)合蛋白使微管與其它細胞結(jié)構(gòu)promotethemicrotubuleactastherailoftransportimprovethemicrotubuleMolecularMotorKinesin:movetowardstheplusendofthemicrotubuleDynein:movetowardstheminusendoftheMyosin:movetowardstheminusendoftheAssemblyof ring(環(huán) coiledring(螺旋帶

DynamicinstabilityMicrotubule-organizing(MTOC,微管組織中心assemblyCa2+:Mg2+:影響微管穩(wěn)定性的colchicine(秋水仙素秋水仙素與未聚合的微管蛋白二聚體結(jié)合,taxol紫杉醇促進微管聚合；穩(wěn)定已聚 Functionsof1.Providemechanicalsupport---maintainthecell2.Transport---Serveastracksforthemovementofcytoplasmicparticlesandorganelles3.Attendthecellmovement:ciliaand4.Attendthecelldivision:chromosome5.Signal cellstructureformedby.2Centriole(中心粒Centrosome:中心體pericentriolarmaterial:中心粒周圍物

一1、Twobarrel-shapedcentriolessituatedatrightanglestooneanother(EM)Centriolescontainnineevenlyspacedfibrils,eachofwhichappearsasabandofthreemicrotubules(triplet).Structurediagram: InitiatestheformationofProduce:newlysynthesize,not二FormciliaandAttendcellMotileorganellesthatprojectfromthesurfaceofavarietyofeukaryoticcellsStructure axoneme(軸絲)basalbody：9問答題（細胞骨架12、簡述微管的4鞭毛二聯(lián)Cytoskeleton(EM)red yellow Smoothmusclecell:redfluorescence--- blue---CulturedRedfluorescence: greenfluorescence:Yellowfluorescence---Culturedmouse

doublet motor(動力蛋白(驅(qū)動蛋白發(fā)動機蛋白單Inastepwise

coiledring plusplusMinusendsMinusends紅紅色表示動力蛋綠色表示驅(qū)動蛋Blueparticles----signalmolecule紫杉醇的 MTOCandmicrotubulemicrotubuledirectthematerial ActasthetransporttrackofkinesinisatetramerconstructedfromtwoidenticalheavychainsandtwoidenticallightAkinesinmoleculehasapairofglobularheadsthatbindamicrotubuleandactasATP-hydrolyzlng,force-generatingafan-shapedtailthatbindscargotobeKinesin驅(qū)動蛋 結(jié)構(gòu)特Kinesinisaplusend-directedmicrotubularmotorEachstepisapproximay8nminlength,whichisalsothespacingbetweentubulindimersalongaprotofilament移動的速度與ATP驅(qū)動蛋白 方Dyneins(動力蛋白)：(+Dyneinisahugeproteinmolecularmassof