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PharmaceuticalanalysisContentdeterminationofvitaminB1tablet---(ultraviolet-visiblespectrophotometry)ContentdeterminationofvitaminB1tablet---(ultraviolet-visiblespectrophotometry)Author:XuYingqianContentsSectionITrainingpurposeSectionIITraininginstrumentandreagentSectionIIITrainingprincipleandstepSectionIVTrainingreportSectionVTrainingprecautionsKeyanddifficultpointsMasterFamiliarPrincipleofcontentdeterminationofvitaminB1tabletbyultravioletspectrophotometryApplicationmethodofultravioletspectrophotometerUnderstandRelationshipbetweenmolecularstructureofvitaminB1anditsultravioletabsorptionSectionITrainingpurposeSectionITrainingpurpose1.1.Trainingpurpose1.Masterthedeterminationofpharmiccontentbyabsorptioncoefficientmethod2.Familiarwiththeprincipleandoperationofultraviolet-visiblespectrophotometer.SectionIITraininginstrumentandreagentSectionIITraininginstrumentandreagent2.1TraininginstrumentandreagentInstrument: Mortar,funnel,100mlvolumetricflask,5mlpipette,smallbeaker,ultravioletspectrophotometer,electronicbalance,etc.Reagent: Hydrochloricacid,vitaminB1tabletSectionIIITrainingprincipleandstepSectionIIITrainingmethodandstep3.1TrainingprinciplePrinciple:ThemoleculeofvitaminB1hasconjugateddoublebondstructure,soitcanproduceultravioletabsorptionatacertainwavelength,andthecontentcanbecalculatedbymeasuringtheabsorbanceofsolutionwithcertainconcentration.SectionIIITrainingmethodandstep3.2Trainingstepb.Method①Take20tabletsoftestproducts,accuratelyweighandgrindthemintofinepowderandthenaccuratelyweighanappropriateamountoffinepowder(equivalentto25mgvitaminB1),andplacethemina100mlvolumetricflask;②Addabout70mlhydrochloricacidsolution(9→1000),shakeitfordissolution,diluteittoscalewithsolventandthenshakeitwellandfilterit;Finally,takesubsequentfiltrateforlateruse;③Accuratelymeasure5mlsubsequentfiltrate,putitina100mlvolumetricflask,andkeepitataconstantvolumewithhydrochloricacidsolution(9→1000);④Determinetheabsorbanceatawavelengthof246nm.SectionIIITrainingmethodandstep3.3CalculationformulaA:Measuredabsorbance;V:Volumeoftestproductthroughinitialdissolution;D:Dilutionratio;:Averagetabletweight(g);m:Samplingamountofthetestproduct(g);Sisthelabeledamount.LabelledamountSectionIIITrainingmethodandstep3.4ReferencestandardThevitaminB1(C12H17ClN4OS·HCl)shouldbe90.0%-110.0%oflabelledamount.ChinesePharmacopoeia[Contentdetermination]Take20tabletsofthisproduct,accuratelyweighthemandgrindthemintofinepowder.Thenaccuratelyweighanappropriateamount(about25mgofvitaminB1),putitina100mlvolumetricflask,addabout70mlofhydrochloricacidsolution(9→1000),shakeitfor15mintodissolvevitaminB1anddiluteittoscalewiththeabovesolvent,shakeitwellandfilteritwithdryfilterpaper.Next,accuratelymeasure5mlofthesubsequentfiltrate,putitinanother100mlvolumetricflask,diluteittothescalewiththeabovesolvent,andshakeitwell.Finally,determinetheabsorbanceatawavelengthof246nmbyUltraviolet-visiblespectrophotometry(AppendixIVA)andcompletethecalculationwiththeabsorptioncoefficient(E1%1cm)ofC12H17ClN4OS·HClas421toobtainthecontent.SectionIVTrainingreportSectionIVTrainingreport4.1TrainingreportOriginalrecordandresultofcontentdeterminationofvitaminB1tabletTestdate

Temperature

Relativehumidity

;Nameoftestproduct

Doseform

Specification

;Manufacturer

BatchNo.

Validity

;Testbasis

Testpurpose

;1.Averagetabletweight(g):weighingrange:Weightoftabletpowder(g):testproductm1=2.Determinationofabsorbance:A1=A2=A3＝3.Contentcalculation:LabelledamountofVitB1tablet%SectionIVTrainingreport4.2TrainingreportMedicinetestreportNameoftestproductManufacturerDoseformSpecificationBatchNo.ValidityPackingInspectednumberTestpurposeTestitemTestdateReportdateTestbasisTestconclusion:SectionVTrainingprecautionsSectionVTrainingprecautions5.1Precautions①Poweronandpreheattheinstrumentfor20min;②Setcorrectwavelengthfordetermination;③TheultravioletabsorptionpeakofvitaminB1varieswiththepHofsolution,sothepHofsolutionshouldbestrictlycontrolled.Summary1.Treatmentandpreparationoftabletsamplesolutio

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