名大personal history ver４abbreviated固有名詞はすべて正式な名稱とし一切

AL履歴書１．Theapplicationshouldbetypewrittenifpossibleorneatlyhandwritteninblockletters.（明瞭に記入すること?)２．NumbersshouldbeinArabicnumerals.（數(shù)字は算用數(shù)字を用いること?)３．AllyearsshouldbewrittenusingtheWesternCalendar.（年號(hào)はすべて西暦とすること４．Propernounsshouldbewritteninfullandnotbeabbreviated.（固有名詞はすべて正式な名稱とし，一切省略しないこと(１．Fullnameinnative □Male（男， (Family (First (Middle InRomanblock ，

(Marital (Family (First (Middle □Married（國(guó)籍PasteaphotographofyourselftakenPasteaphotographofyourselftakenwithinthepast6months.Writeyournameandnationalityinblocklettersonthebackofthephoto. （宗教 な４．Dateofbirth(生年月日 Month（月 Age（年齢 E-mail ７．Fieldofspecializationstudiedinthepast（BeasdetailedandspecificasImajorfoodqualityandsafetyatTianjinUniversityofScienceandTechnology.Inresearchprograms,Istudied(1)influenceandmechanismofthePurplesweetpotatonutrientstoDrosophila’slifespan,and(2)preparationandpurificationofantimicrobialpeptidesandantibacterialexperiments.Ilearnedavarietyofskillsingeneengineering,proteinpurificationandysis,enzymeactivityassays,handlingofinsects,microbialbioassays,etc.８．ProposestudyprograminJapan;State,inmorethan600words,thedetailsofyourmajorfieldofstudyandstudyprogram.Thisitemwillbeusedasoneofthemostimportantreferencesforselection.Statementmustbetypewritteninblockletters.Additionalsheetsofpapermaybeattachedifnecessary.WriteinJapaneseifyouhaveJapaneselanguageability.ⅰ）FieldofStudyindetailIwillstudymolecularandcellbiologicalstudiesoncalcium-bindingproteinsandtheirinctingproteinsinthelaboratoryofMolecularandCellularRegulation,DepartmentofAppliedMolecularBiosciences,GraduateSchoolofBioagriculturealSciences,NagoyaStudyProgramindetailIntroduction―ALG-2isa22-kDaCa2+-bindingproteincontainingfiveseriallyrepetitiveEF-handmotifs,andbelongstothepenta-EF-hand(PEF)family.Ithasbeenreportedtobeinvolvedinapoptosis,cancerdevelopment,signaltransduction,membranetraffickingandpost-transcriptionalcontrol,howevertheprecisemechanismsremainunclear.ALG-2inctswithvariousproteinsandalsofunctionsasanadaptorproteininacalcium-dependentway.Afterbindingtocalciumion,ALG-2changesitsconformationandinctswithvariousproteins,suchasSec31A,peflin,ALIX,annexinsA11andA7,HD-PTP,TSG101,VPS37andetc.MostoftheALG-2-inctingproteinscontainPro-richregions.TwoALG-2-bindingmotifs:PPYPXnYP(type1motif)and[PΦ]PX[PΦ]G[FW]Ω(Φ,hydrophobic;Ω,largesidechain;[PΦ],PorΦ;[FW],ForW;type2motif)havebeenidentifiedbycomparisonofdifferentbindingsitesofALG-2-inctingproteins.BasedonALG-2-bindingmotifs,searchingfornewinctingproteinsofALG-2andinvestigatingfunctionsoftheseALG-2-inctingproteinsmaygiveusabetterunderstandingofthemechanismsinwhichALG-2isinvolved.InsilicosearchfornewALG-2-inctingproteinsbasedontype2ALG-2-bindingmotif:ExpressionofcandidateproteinsinHEK293TcellsandverificationofCa2+-dependentinctionsbetweenALG-2andcandidatesbyGST-pulldownandco-immunoprecipitation(co-IP)followedbyWesternblot(WB)andfar-Westernblot(FW).InvestigationofsubcellularlocalizationofALG-2-bindingproteinsandeffectsofoverexpressionorknockdownofALG-2-inctingproteinsoncellsurvival,signalingcascade,proteintransport,transcriptionandpost-transcriptionalevents.MakiM,SuzukiH,ShibataH.StructureandfunctionofALG-2,apenta-EF-handcalcium-dependentadaptorprotein.SciLifeSci,2011,54,770-779.TakahashiT,KojimaK,ZhangW,etal.Structuralysisofthecomplexbetweenpenta-EF-handALG-2ProteinandSec31ApeptiderevealsanoveltargetrecognitionmechanismofALG-2.IntJMolSci,2015,16,3677-3699.HelmJR,BentleyM,ThorsenKDetal.Apoptosis-linkedgene-2(ALG-2)/Sec31inctionsregulateendosmicreticulum(ER)-to-Golgitransport.JBiolChem,2014,289,23609-23628.laCourJM,SchindlerAJ,BerchtoldMW,SchekmanR.ALG-2attenuatesCOPIIbuddinginvitroandstabilizestheSec23/Sec31Acomplex.PLoSOne,2013,8,e75309.ShibataH,KanadomeT,SugiuraHetal.AnewroleforannexinA11intheearlysecretorypathwayviastabilizingSec31Aproteinattheendosmicreticulumexitsites(ERES).JBiolChem,2015,290,4981-4993.９．Educationalbackground（學(xué)歴YearandMonthofEntranceandAmountoftimespentattheDiplomaorDegreeawarded,Majorsubject From1999、To2004、5（年（月NameFrom2004、To2008、4（年（月From2008.To2011、3（年（月HigherLocationFrom2011、4（年UndergraduateTo2015、（月（年Graduate（月Totalyearsofschoolingmentioned16（年１０．Statethetitlesorsubjectsofbooksorpapers(includinggraduationthesisauthoredbytheapplicantifanywiththenameandaddressofthepublisherandthedateofpublication.（著書，論文（卒業(yè)論文を含む?）があればその題名，名，年月日，場(chǎng)所を記すこと?） softhosepaperstothisGraduationproject:PreparationandpurificationofantimicrobialpeptidesandantibacterialDesigntheprimer.UsePCRtoobtaincompletegenesequenceofHispidalin.The binantsmidsweretransformedintobacterialstrain,induceexpressiontodeterminetheoptimalinductionconditions.Thennickelaffinitychromatographywasusedforseparationandpurificationbinantfusionprotein.Agarwelldiffusionexperimentisusedtoexaminetheantibacterialactivityof:Typeof１２．Japaneselanguagebackground,ifNameandaddressofPeriodof Year(年 NameofJapaneselanguageproficiency:EvaluateyourlevelandfillinwithanXwhereappropriateinthefollowing××