ATWLPPRAANLLMAAS-生命科學試劑-MCE

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEATWLPPRAANLLMAASCat.No.:HY-P10832CASNo.:1228447-26-4分?式:C??H???N??O??S分?量:1682.98Sequence:Ala-Thr-Trp-Leu-Pro-Pro-Arg-Ala-Ala-Asn-Leu-Leu-Met-Ala-Ala-SerSequenceShortening:ATWLPPRAANLLMAAS作?靶點:Apoptosis;Apoptosis;Ras;Raf;MEK;ERK;Caspase;PARP;Bcl-2Family作?通路:Apoptosis;GPCR/GProtein;MAPK/ERKPathway;StemCell/Wnt;CellCycle/DNADamage;Epigenetics儲存?式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY?物活性ATWLPPRAANLLMAAS?種嵌合肽，具有抗?管?成和強效的抗腫瘤作?。ATWLPPRAANLLMAAS可以抑制?肝細胞癌細胞的增殖、活?、遷移和侵襲，并誘導細胞凋亡(apoptosis)[1][2]。體外研究ATWLPPRAANLLMAAS(V3)(25-200μM;12-24h)caninhibittheproliferation,viability,migrationandinvasionofhumanHCC(SMMC-7721,Huh-7)cellsbyinhibitingtheRas/Raf/MEK/ERKsignalingpathway,however,nosignificanteffectwasobservedinHL-7702cells[2].ATWLPPRAANLLMAAS(200μM;24h)increasesapoptosisanddecreasesproteinlevelsofH-RAS,phosphorylated(p)-RAF,p-MEK,andp-extracellularsignal-regulatedproteinkinase(ERK)inhumanHCC(SMMC-7721,Huh-7)cells[2].CellMigrationAssay[2]CellLine:HL-7702,SMMC-7721,Huh-7Concentration:25,50,100,200μMIncubationTime:12h,24hResult:InhibitedthemigrationofSMMC-7721andHuh-7cellsinadose-dependentmanner,buthadnoeffectonHL-7702cells.1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEApoptosisAnalysis[2]CellLine:SMMC-7721,Huh-7Concentration:200μMIncubationTime:24hResult:Remarkablyincreasedtheapoptoticindex,proteinexpressionsofcleavedcaspase-3,8,9,cleavedPARP,aswellasBax/Bcl-2andBad/Bcl-xlratios.體內(nèi)研究ATWLPPRAANLLMAAS(160-480μg/kg;s.c.;oncedailyfor7days)cansignificantlyinhibitthegrowthofmousexenograftsarcomaS180andH22livercancerxenograftsinadose-dependentmanner[1].ATWLPPRAANLLMAAS(200μg/kg;s.c.;oncedailyfor4weeks)inhibitsthegrowthofhumanHCCxenograftsbydownregulatingangiogenesisandupregulatingapoptosis[2].REFERENCES[1].WuD,etal.Anti-tumoreffectsofanovelchimericpeptideonS180andH22xenograftsbearingnudemice[J].Peptides,2010,31(5):850-864.[2].WuD,etal.PeptideV3inhibitsthegrowthofhumanhepatocellularcarcinomabyinhibitingtheRas/Raf/MEK/ERKsignalingpathway[J].JournalofCancer,2019,10(7):1693.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forrese