從零學(xué)測(cè)序之轉(zhuǎn)錄組與長鏈非編碼rna分析視頻講義long noncoding rnas1st

1、Long Noncoding RNAs: Genomics, Functions, Methodologies, Modes of Actions Wei Guifeng, Institute of Biophysics, Chinese Academy of Sciences RNA Biology 1941 One Gene, One Enzyme 1953 DNA, double helix 1958 Crick, Central Dogma 1961 mRNA discovery Jacob l Development and Lineage commitment; l Immunol

2、ogy; l Neurogenetics; l Cancer progression (Tumor Suppressor or Oncogene); Long Noncoding RNAs Histone modification (HOTAIR), DNA methylation, high-order chromatin structure (Firre), transcription factor binding activity (RMST with SOX2), decoy model (PANDA), Long Noncoding RNAs lHOTAIR affects expr

3、ession of the HOXD gene family; lXIST is crucial for the X chromosome inactivation; llincRNA-ROR affects reprogramming efficiency; lAir is crucial for imprinting control at Igf2r locus; lNRON affects NFAT transcription factor activity; lTug1 affects retina development through the regulation of the c

4、ell cycle; lHOTTIP activates the expression of HOXA gene family through physically interacting with WDR5; l Several well-characterized lncRNAs with detailed molecular mechanisms Long Noncoding RNAs = 30% of lincRNAs physically interact with chromatin regulatory proteins. Mitchell Guttman et al. Nature 2011 Four principles of nucleic acid and protein interactions ceRNA (miRNA