常用凝膠參數(shù)

1、Fpg常用凝膠技術(shù)參數(shù)1.葡聚糖凝膠某些技術(shù)數(shù)據(jù)種類干顆粒直徑()分子量分級范圍床體 積毫 升/克 干分 子篩得水值溶脹 最少 平衡時間(h)柱頭壓力(kPa)(2.5cm直徑柱)肽及球形 蛋白質(zhì)葡聚糖(線 性分子)室 溫沸水浴SephadexG-1040 120700700231.0 土 0.131SephadexG-1040 120150015002.53.51.5 土 3.531SephadexG-25粗級100300( 5100 目)50 1501,000 100中級( 100200 目)5,0005,000461.5 土 0.26220細(xì)級800( & 200400 目)超細(xì)

2、1040SephadexG-50粗級100 200中級50 1501,500 500細(xì)級20 8030,00010,0009115.0 土 0.362超細(xì)1040SephadexG-7540 1203,000 1,000 超細(xì)104070,000950,00012157.5 土 0.52433.92 15.86SephadexG-10040 1204,000 1,000 超細(xì)10401,500,00050,000152010.0 土 1.04852.35 9.41SephadexG-15040 1205,000 1,000 2030超細(xì)1040400,000150,000182215.0 土

3、1.57250.88 3.53SephadexG-20040 1205000100030401040800,000200,000202520.0 土 2.07250.39 1.572.聚丙烯酰胺凝膠CD技術(shù)數(shù)據(jù)型號排阻下限（分子量）分級分離范圍（分子量）膨脹后床體積（ml/g干凝 膠）膨脹所需最少時間（室溫,h）Bio-gel-P-21,6002002,0003.824Bio-gel-P-43,6005004,0005.824Bio-gel-P-64,6001,000 5,0008.824Bio-gel-P-1010,0005,000 17,00012.424Bio-gel-P-3030,00

4、020,000 50,00014.910 12Bio-gel-P-6060,00030,000 70,00019.010 12Bio-gel-P-100 100,00040,000 100,00019.024Bio-gel-P-150 150,00050,000150,00024.024Bio-gel-P-200 200,00080,000 200,00034.048Bio-gel-P-300 300,000100 400,00040.0483.瓊脂糖凝膠技術(shù)數(shù)據(jù)型號瓊脂糖含量%（w/vy排阻下限（分子量）分級分離。）范圍（分 子量）生產(chǎn)廠家Sepharose 4B40.3 X 1063X 1

5、06PharmaciaSepharose 2B22X 106 25X 106Sagavac 10102.5 X 1051X 104 2.5 X 105SeravacSagavac 887X 1052.5 X 1047X 105Sagavac 662X 1065X 104 2X 106Sagavac 4415X 1062X 105 15X 106Sagavac 22150X 1065X 105 15X 107Bio-gel A-0.5M100.5 X 106<1X 1040.5 X 106Bio-gel A-1.5M81.5 X 106<1X 1041.5 X 106Bio-gel

6、A-5M65X 1061X 104 5X 106Bio-gel A-15M415X 1064X 104 15X 106Bio-RadBio-gel A-50M250 X 1061X 105 50 X 106Bio-gel A-150M1150X 1061X 106 150X 1064.各處凝膠所允許最大操作壓凝膠取大靜水壓（kPa）SephadexG-109.8G-159.8G-259.8G-509.8G-754.95 .瓊脂糖凝膠濃度與線性DN的辨范圍凝膠濃度（線性DN職度（bp）0.51000300000.7800120001.0500100001.240070001.520030002.

7、0|50 20006 .染料在變性聚丙烯酰胺凝膠中遷移速度凝膠濃瘦:(%漠酚藍(lán)二甲苯青FF5.035bp140bp6.026bp106bp8.019bp75bp10.012bp55bp20.08bp28bp7.染料在非變性聚丙烯酰胺凝膠中遷移速度凝膠濃度（漠酚藍(lán)二甲苯青FF3.5100bp460bp5.065bp260bp8.045bp160bp12.020bp70bp15.015bp50bp20.012bp45bp七、氨基酸特性八、遺傳密碼UCAGUUUPheUCUSerUAUTyrUGUGysUUUCPheUCCSerUACTyrUGGGysCUUUALeuUCASerUAAl終止（赭石）

8、UGA* （乳曰）AUUGLeuUGGSerUA(孩止（琥珀）UGGTrpGCUULeuCCUProCAUHisCGAArgUC CUCLeuCCCProCAC:HisCGCArgC密碼子第二位密碼子 第三 位（3 端'）碼0一 ) 密子第位端CUALeuCCAProCAAGlnCGAArgACUGLeuCCGProCAGGlnCGCArgGAUUIleACUThrAAUAsnAGUSerUAUCIleACCThrAACAsnAGCSerqAAUAIleACAThrAAALysAGAArgAAUGMetACGThrAAGLysAGCArgGGUUValGCUAlaGAUAspGGUGl

9、yuGUCValGCCAlaGACAspGGCGly日GGUAValGCAAlaGAAGluGGAGlyAGUGValGCGAlaGAGGluGGGGlyG九、常用酸堿技術(shù)參數(shù)NaOH40.0019.10 763501.530 52.42.75111 101.110 363.4溶質(zhì)分子式分子量mol/L g/L重量(為比重配制mol/L溶液加入量(ml/L )冰乙酸CHCOOH60.0517.401045 99.51.05057.5乙酸60.056.27376361.04559.5甲酸HCOOH46.0223.40080 901.20012.7鹽酸HCl36.5011.60124361.180

10、36.22.90105101.050 344.8硝酸HNO63.0215.99 1008 711.420 62.514.90 938671.400 67.113.30 :沼7611.370 75.21.常見市售酸堿濃度HclOa100.50 11.65 1172 701.670 85.89.20923601.540108.7磷酸HPO80.0018.101445 851.700 55.2硫酸岳Q98.1018.00776 961.840 55.6氫氧化俊NHOH35.0014.80 :251280.898 (57.6氫氧化鉀KOH56.1013.50 ；757501.520M.11.94109 101.090 515.52 .各種濃度酸堿貯存液CD近似 pH值溶質(zhì)1N30.1Na0.01Na0.001Na