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1、微生物培養(yǎng)基大全(國(guó)外翻譯版)146 種培養(yǎng)基配方供參考。146 種培養(yǎng)基配方THECOMPOSITIONOFMEDIA培養(yǎng)基及成分I.AcetobacterMedium(醋酸菌培養(yǎng)基)Glucose(ffi 萄糖)100gYeasstextract(孝母膏)10gCaCO320gAgar(xx)15gDistilledwater(蒸儲(chǔ)水)1000mlAdjust(調(diào))pHto6.8適用范圍:惡臭醋酸桿菌混濁變種2.NutrientAgar(營(yíng)養(yǎng)肉汁瓊脂)Pepton(蛋白月東)5gBeefextract(牛肉膏)30gNaCl5gAgar(xx)15gDistilledwater(蒸儲(chǔ)水)A
2、djust(調(diào))pHto7.07.2Note:WhencultivationofBacillus, 5mgoftoMnSO5 / 654.H2Omaybeadded .Itisfavorabletopromotesporeformation .適用范圍:產(chǎn)氣氣桿菌、糞產(chǎn)堿桿菌、蠟狀芽孢桿菌、蠟狀芽孢桿菌蕈狀變種、地衣形芽孢桿菌、巨大芽孢桿菌、多粘芽孢桿菌、塵埃芽孢桿菌、短小芽孢桿菌、嗜熱脂肪芽孢桿菌、枯草芽孢桿菌、枯草芽孢桿菌深黑變種、蘇云金芽孢桿菌、蘇云金芽孢桿菌蠟螟亞種(青蟲菌)、蘇云金芽孢桿菌戈?duì)査沟伦兎N、蘇云金芽孢桿菌猝倒亞種、產(chǎn)氨短桿菌、黃色短桿菌、谷氨酸棒狀桿菌、北京棒桿菌、大腸埃
3、希氏菌(大腸桿菌)、銅綠假單胞菌(綠膿桿菌)、凸形假單胞桿菌、熒光假單胞菌、彎曲假單胞菌、惡臭假單胞菌、假單胞桿菌、藤黃八疊球菌、亞黃八疊球菌、尿素八疊球菌、金黃色葡萄球菌、運(yùn)動(dòng)發(fā)酵單孢菌3.AzotobacterMedium(固氮菌培養(yǎng)基)KH2PO40.2gK2HPO40.8gMgSO4.7H2O0.2gCaSO4.2H2O0.1gNa2MoO4.2H2OTrace微量)Yeastaxtract既孝母膏)0.5gMannitol(甘露醇)20gFeCl3Tract量)Distilledwater(蒸儲(chǔ)水)1000mlAgar密月旨)15gAdjust(調(diào))pHto7.2適用范圍:固氮菌、膠
4、質(zhì)芽孢桿菌4 .CornMealMedium(玉米粉培養(yǎng)基)Maizeflour(玉米粉)5gPeptone(蛋白月東)0.1gGlucose(ffi 萄糖)1gTapwater(自來水)1000mlNote:Boilthemixtureinautoclaveat121 C for1hr.distributethemediuminto18 x 18mmtu bes,eachcontains10mloftheliquid,thenautoclaveat121 for1hr .again(15 磅蒸煮 1 小 時(shí),分裝入18 x 18毫米試管,每管深度達(dá)6厘米。15磅再次滅菌1小時(shí)。)5 .Lac
5、tic-bacteriaMediumI(乳酸菌培養(yǎng)基 I)Yeastextract(酵母膏)7.5gPeptone(蛋白月東)7.5gGlucose(ffi 萄糖)10gKH2P042gTomatojuice(西紅柿汁)100mlTween(吐溫)800.5mlDistilledwater( 蒸餾水 )900mlpH7.0適用范圍:植物乳桿菌(胚芽乳桿菌)、嗜熱乳酸鏈球菌6 .Lactic-bacteriaMidium H (乳酸菌培養(yǎng)基 H )Lacto-caseinpeptone俘L酪蛋白月東)10gBeefextract(蛋白月東)10gYeastextract(酵母膏)5gGlucos
6、e葡萄糖)5gTween(吐溫)801gK2HPO42gNa-acetate(醋酸鈉)5gDiaminecitrate(檸檬酸二胺)2gMgSO4.7H2O0.2gMnSO4.H2O0.05gDistilledwater( 蒸餾水 )1000mpH6.5 6.8適用范圍:植物乳桿菌(胚芽乳桿菌)7.PeotoneGlucoseYeastextractMediumPGY(蛋白月東、酵母膏、葡萄糖培養(yǎng) 基)Peptone (蛋白月東)10gYeastextract (酵母膏)5gGlucose (葡萄糖)IgDistilledwater (蒸儲(chǔ)水)1L1.1 lycerolAgar (甘油瓊脂)P
7、eptone (蛋白月東)5gBeefextract (酵母膏)3gGlycerol (甘油)20gTopwater (自來水)1000mlAgar( xx) 15gpH1.2 1.39.Rhizobiummedium (根瘤菌培養(yǎng)基)AS9Yeasteztract (酵母膏)1gSoileztract (土壤浸提液)200mlMannitol (甘露醇)10gAgar (瓊脂)15gDistilledwater (蒸儲(chǔ)水)800mlpH7.2Note:Soilextract:Suspend50gfinelyanddriedgardonsoilin200mloftapwater.Autocla
8、veat121 for1h r.Decantthroughcotton- cloth,fillerthoughpaper,makeupvolumeto200ml.Resterilizefor20minutesat121 ,then mixedwithothringredientsanddistributed.( 土壤浸提液的制法:取土壤 50 克,加水200 毫升, 15 磅蒸煮 1 小時(shí),經(jīng)濾紙過濾后加水補(bǔ)足到200 毫升。)適用范圍:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根 瘤菌1.1 Mannitol
9、Agar(甘露醇瓊月旨)Yeastextract(酵母膏)5gPeptone(蛋白月東)3gMannitol(甘露醇)25gAgar晾脂)15gDistilledwater( 蒸餾水)1000ml1.2 GlucoseAsparagine (葡萄糖、天門冬素瓊脂)Glucose (葡萄糖)10gAsparagine (天門冬素)0.5gK2HPO40.5 gWater (水)1000mlpH40.640.7適用范圍:刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)12.Gause ' sSyntheticAgaU氏合成一號(hào)瓊脂)KNO31gSolublestarch (可溶性淀粉)2
10、0gK2HPO40.5gMgSO4.7H2O0.5gNaCl0.5gFeSO7 / 6540.01gAgar (瓊月旨)20gwater (水)1000mlpH1 .22 .4適用范圍:刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)、白黃鏈霉菌、白色鏈霉菌、抗生鏈霉菌、雙重輪絲鏈霉菌、產(chǎn)色鏈霉菌、燼灰鏈霉菌、天藍(lán) 色鏈霉菌、滅蚊鏈霉菌、紅霉素鏈霉菌、青色鏈霉菌、球孢鏈霉菌、淺灰鏈霉 菌、灰色鏈霉菌、吸水鏈霉菌、淡紫灰鏈霉菌、黃色長(zhǎng)孢鏈霉菌、藤黃色鏈霉 菌、細(xì)黃鏈霉菌、黑化鏈霉菌、玫瑰色鏈霉菌、華美鏈霉菌、嗜熱鏈霉菌、委內(nèi)瑞拉鏈霉菌、紫色直絲鏈霉菌、紫色鏈霉菌、綠色鏈霉菌13 .WortAg
11、ar (xx 汁 xx)Dilutetheworld(withouthop)to12Brix.Add15garinto1000mlofthedilutedword.M elttheagarbyheating,thendistributethemediumintotubes.Autoclaveat110for30minute s.(將發(fā)酵啤酒的原料(未加酒花),稀釋至 12柏林,加瓊脂15克,溶化后分 裝。 15 磅滅菌 30 分鐘。)適用范圍:克魯斯假絲酵母、郎比可假絲酵母、解脂假絲酵母、馬其頓假絲酵母、擬熱帶假絲酵母、粗壯假絲酵母、皺褶假絲酵母、熱帶假絲酵母、產(chǎn)朊假絲酵 母、阿舒假囊酵母、白
12、地霉、果香地霉、地霉屬、異常漢遜酵母、異常漢遜酵 母變種、阿拉伯糖醇漢遜酵母、施氏漢遜酵母、菅囊酵母、伯頓畢赤酵母、膜 醭畢赤酵母、粘紅酵母、小紅酵母、膠紅酵母、深紅酵母、卡爾斯伯酵母、釀 酒酵母、椰園釀酒酵母、發(fā)酵性酵母、洛格酵母、羅斯酵母、魯氏酵母多形魯 氏酵母、威爾酵母、酵母、路德類酵母、栗酒裂殖酵母、擲抱酵母、貝雷絲孢 酵母、皮狀絲孢酵母、糙孢曲霉、無殼曲霉、鮮橙曲霉、紅曲霉、紫紅曲霉、 紅色紅曲霉、紅曲霉菌14 .PotatoDextroseAgarPDA馬鈴薯、葡萄糖瓊脂)9 / 65Potatoextract(馬鈴薯汁)1000mlDextrose(glucose)葡萄糖)20g
13、Agar(xx)20gNote:Potatoextract:Slicepotatoesthin.Add1000mlofwater,immediatelytopreventOxidtionofjuiceand boiluntilsoft.(approximately30min.).Filterthroughcotton- cloth.Autoclaveat115 for20minutes. (注:取去皮馬鈴薯200 克,切成小塊,加水1000 毫升煮沸30 分鐘,濾去馬鈴薯塊,將濾液補(bǔ)足至1000毫升,加葡萄糖20克,瓊脂15克,溶化后分裝,15磅滅菌 30 分鐘。)適用范圍:酵米面假單胞菌(酵
14、米面黃桿菌)、白色鏈霉菌、燼灰鏈霉菌、青色鏈霉菌、球孢鏈霉菌、灰色鏈霉菌、龜裂鏈霉菌、傘枝梨頭霉、雅致放射毛 霉、棒曲霉、米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛殼、長(zhǎng)刺毛 殼、橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、束狀刺盤孢、新月彎孢霉、奇異 翅孢殼、地生翅孢殼、串珠鐮孢、尖鐮孢、盤長(zhǎng)孢菌(魯保一號(hào))、銀白楊盤長(zhǎng)孢、 玉蜀黍長(zhǎng)蠕孢、深黃被孢霉、小被孢霉、多頭被孢、拉曼被孢霉、葡萄色被孢霉、生 香毛霉、卷枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢 毛霉、總狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青 霉、棉鈴蟲擬青霉、擬青霉、球形阜孢
15、、白腐菌、少根根霉、華根霉、科恩根霉、戴爾根霉、日本根霉、爪哇根霉、米根霉、點(diǎn)頭根霉、綠穗霉、簇抱匍柄霉、雅致 枝霉、康寧木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢15 .Czapek ' sAg嘉氏瓊脂)Sucrose (蔗糖)30gNaNO33gMgSO4.7H2O0.5gKCl0.5gFeSO4.7H2O0.01gK2HPO41gAgar儂月旨)13gDistilledwater(蒸儲(chǔ)水)1000mlpH16 2適用范圍:紅霉素鏈霉菌、洋蔥曲霉、金頭曲霉、泡盛曲霉、泡盛曲霉煙色變種、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、無花果曲霉、黃柄曲霉、黃曲霉、煙曲霉、構(gòu)巢曲霉、黑曲霉、赭曲
16、霉、米曲霉、寄生曲霉、醬油曲霉、土曲霉、宇佐美曲霉、焦曲霉、雜色曲霉、溫特曲霉、阿達(dá)青霉、產(chǎn)黃青霉、黃綠青霉、桔青霉、頂青霉、園弧青霉、繩狀青霉、灰黃青霉、島青霉、淡紫青霉、特異青霉、赭綠青霉、產(chǎn)紫青霉、婁地青霉、小刺青霉、蕁麻青霉、16.ConcentratedSucroseCzapek 濃糖Ss(i瓊月旨)Sucrose 糖)200gNaNO33gMgSO4.7H2O0.5gKCl0.5gFeSO4.4H2O0.01gK2HPO41gAgar儂月旨)13gDistilledwater(蒸儲(chǔ)水)1000ml適用范圍:xx曲霉17.SyntheticPotatoMedium(綜合馬鈴薯培養(yǎng)基)
17、20%Potatoextract(馬鈴薯汁)1LGlucose葡萄糖)20gKH2PO43gMgSO4.7H2O1.5gVitanibB1(硫胺素)Trace微量)Agar儂脂)15gpH6適用范圍:米曲霉、醬油曲霉、棲土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛客、長(zhǎng)刺毛殼、球毛殼、橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、多主枝孢、束狀刺盤孢、葫蘆科刺盤孢、新月彎孢霉、奇11 / 65異翅孢殼、地生翅孢殼、串珠鐮孢、蝕脈鐮孢霉、盤長(zhǎng)孢菌(魯保一號(hào))、銀白楊盤長(zhǎng)孢、瓶毛殼、金龜子綠僵菌、生香毛霉、卷枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總
18、狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬青霉、淡紫色擬青霉、擬青霉、白腐菌、少根根霉、根霉屬的菌、華根霉、科恩根霉、戴爾根霉、臺(tái)灣根霉、日本根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、點(diǎn)頭根霉、三寶壟根霉、根霉屬的種、綠穗霉、康寧木霉、木素木霉、擬康氏木霉、木霉、粉紅木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢、雙孢蘑菇、大肥菇、發(fā)光假蜜環(huán)菌、木耳、皺木耳、毛木耳、大禿馬勃、長(zhǎng)根菇、金針菇、棘托竹蓀、短裙竹蓀、短裙竹蓀東北變種(暫定)、長(zhǎng)裙竹蓀、白鬼筆、無裙竹蓀、寬鱗大孔菌、凸圓靈芝(白芝)、紫芝、靈芝(紅芝,赤芝,日本靈芝,韓國(guó)靈芝)、灰樹花、猴頭菌、
19、香菇、粗毛斗菇、潔麗香菇、豹皮菇、玉蕈、真姬菇、安絡(luò)小皮傘菌、鬼毛針、黑柄皮傘菌、尖頂羊肚菌、圓錐羊肚菌、高羊肚菌、黑脈羊肚菌、羊肚菌、滑菇、光帽黃傘、尖鱗黃傘、金頂側(cè)耳、榆黃磨、金頂磨、白黃側(cè)耳、紫抱平菇、姬菇、裂皮側(cè)耳、擬囊體仰耳 (鮑魚菇)、真線仰耳短抱變種、真線側(cè)耳、扇形平菇、扁形平菇、佛羅里達(dá)無抱子仰耳、佛羅里達(dá)仰耳、蛭菇、糙皮仰耳、平菇、貝形側(cè)耳、鷹翅菌、肺 形側(cè)耳、柳樹菌、樹窩、扁柄仰耳、美味仰耳、紫抱仰耳、粉褶仰耳、粉紅側(cè) 耳、鳳尾菇、漏斗狀側(cè)耳、榆干仰耳、大榆磨、仰耳、茯苓、黑菇、煙色紅 菇、裂褶菌、雞縱、金耳、黃木耳、銀耳、白木耳、紫晶口磨、銀絲菇、絲蓋 小包腳菇、草菇18
20、 .CornMealMedium(玉米粉培養(yǎng)基)Mixcornmealwithwater20:100(W/V)well .Distributethemixtureinto100mlbottle s,eachbottlecontainsabout30mlofthemixture .Autoclaveat121 for30minutes .(20% 玉米粉裝瓶,15磅滅菌30 分鐘。 )19 .FilterPaperMedium (濾紙培養(yǎng)基)( NH4) 2SO41gKH2PO41gMgSO0.7gNaCl0.5gDistilledwater (蒸儲(chǔ)水)1000mlAstripofilterpa
21、per (濾紙條)6 x1cm (厘 米)20.SoybeanCakeMealAga遁豆餅粉瓊月旨)10%infusionofsoybeancakemeal(10%|豆餅粉浸汁)1000mlGlucose(SJ萄糖)10gNaCl2.5g21. Sawdust, Wheat-bran Medium (木屑、麩皮培養(yǎng)基)Sawdust of broad leaf tree 75g Wheat bran 25gPreparation:Mix these two ingredients well . Add appropriate water and blend (the amount of wat
22、eradded may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick ahole on the filling, put the cotton pulg in bottle mouth .殼斗科植物或闊葉樹的木屑75 克麩皮 25 克制法:加水適量充分混勻,水量以手捏時(shí)指間有水珠為宜,裝瓶并壓緊,在中心處扎一小洞,堵上棉塞,20 磅滅菌 1 小時(shí)。22. Pine Block or Pine Sowdust Medium (松木條或松木屑培養(yǎng)基)1. Cut
23、 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbingsuger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbsfor 1 hr . (W 10 x 2 x 1 厘米的松木條 浸泡在 1-2%的蔗糖水中。使木條吸足糖水,裝瓶不宜過緊,用紙包好,20 磅滅菌1 小時(shí)。 )2. Pine Sawdust
24、MediumPine Sawdust 松木屑)75% Rice bran 維糠)23-24%Socrose 蔗糖)1-2% Water (appropriate)水(適量)Autoclave at 20 lbs for 1 hr.(20 磅滅菌 1 小時(shí))23. Rice Straw. Dung Medium 稻草、馬糞培養(yǎng)基()Dried horse dung (or cow dung)(干馬糞或牛糞) 65%Died straw (干稻草)35% Urea (尿素)0.5%Calcium superhosphate (過磷酸鈣)0.7% Gypsum powder (石膏粉)1%Ammon
25、ium sulphate (硫酸鏤)0.3%Note:Add appropriate water, pile them up and cause them fermentation. (水適量 進(jìn)行堆積發(fā)酵)24. Straw Rice Bran Medium (稻草、米糠培養(yǎng)基)Dried straw (干稻草)75-80% Rice bran (米糠)20-25%Note:Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12hr. Allow the stra
26、w fully (the amount of water added may be decided by kneading with fingers. It is rightwhen a drop of water appeared between fingers). ( 注 :選無霉稻草,切成長(zhǎng)約3 厘米的小段,用清水浸泡12 小時(shí),使充分吸水,然后傾去水,加米糠和水,水量以用手捏時(shí)指間有水珠為宜。)25. Rice Medium (米飯培養(yǎng)基)Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoc
27、lave at 121c for 30 minutes (orf川 2g of rice into a 15 x 150mm tube). Add 6 ml of water. Autoclave at 121 for 30 minutes. (取大米10克,入 50毫升椎形瓶中,水20-30毫升于15磅滅菌30分鐘即成,或取大米2克,裝入15x150毫米試管中,加 水 6 毫升,于15 磅滅菌 30 分鐘。 )26. Filter Paper Medium (濾紙條培養(yǎng)基)(NH4)2SO4 1g KH2PO4 1gMgSO4.7H2O0.5g Yeast Extract孝母膏)0.1gDi
28、stilled Water (蒸餾水 ) 1000mlStrip of filter paper 1 x 7 cm (濾紙條 1 x 7 厘米)Note:Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml ofthe broth). Autoclave at 121 for 30 minutes. Then put one or two sterilized strips of filter paper into atubeaseptically.( 將上述
29、溶液分配成溶液后 分裝入試管,每管1 毫升, 15 磅滅菌 30 分鐘。再將滅菌的濾紙條(1 條或 2條)投入試管中即好使用。)27. Yeast Extract-Medium Extract Agar I醉母膏、麥芽膏瓊脂)Yeast extract (酵母膏) 10g Mall extract (麥芽膏) 10gGlucose (葡萄糖) 4g Distilled water (蒸餾水) 1000mlAgar (xx) 20g pH7.0適用范圍:紅霉素鏈霉菌28. Yeast Extract Peptone(酵母膏、蛋白月東瓊脂)Yeast extract (酵母膏)1g Multi-p
30、eptone (多蛋白月東)2gBeef extract (牛肉膏)1g Glucose(葡萄糖)10gAgar (瓊月旨)20g Distilled water (蒸儲(chǔ)水)1LpH7.029. Glucose Yeast Extract Starch Agar葡萄糖、酵母、淀粉瓊脂)Glucose (葡萄糖)10g Yeast extract (酵母膏)10gStarch (淀粉)10g NaCl 5gCaCO3 3g Agar(瓊脂)20gDistilled water (蒸儲(chǔ)水)1L pH7.030. Wheat Bran Medium (秋皮培養(yǎng)基)Wheat bran (秋皮)36g
31、 (NH4) 2HPO4 10gK2HPO40.2g MgSO4。 7H2O0.1gAgar (瓊月旨)20g Distilled water (蒸儲(chǔ)水)1LpH7.031. Yeast Sporulation Medium (酵母菌產(chǎn)生子囊孢子培養(yǎng)基)(1) Kleyn MediumKH2PO40.012% K2HPO40.02%Sodium acetate (醋酸鈉)0.5% Glucose 葡萄糖()0.062%NaCl0.062% Peptone (蛋白胨)0.25%Biotin (生物素)2 g% Ag瓊脂)2%Trace element solution (混合鹽類) 1ml/10
32、0mlNote:Trace element solution (混合鹽類溶液):MgSO4.7H2O0.4%, NaCl0.4%, CuSO4.5H2O0.002%,MnSO4.4H2O0.2%, FeSO4.4H2O0.2%(2) Gorodkowa MediumGlucose (葡萄糖)0.1% Peptone (蛋白胨) 1%NaCl0.5% Agar (xx) 2%(3) McClary MediumGlucose (葡萄糖)0.1% KCl0.18%Yeast juice (酵母汁)0.25% Sodium actate 醋酸鈉()0.82%Agar (xx)1.5%32. BPY
33、Medium (BPYg 養(yǎng)基)Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10gNaCl 5g Yeast extract 酵母膏 () 5gGlucose (葡萄糖) 5g Agar (瓊脂) 1-2%Distilled (蒸餾水 ) 1000ml pH7.0適用范圍:大腸xx菌(大腸桿菌)、惡臭假單胞菌33. LB Medium (LB培養(yǎng)基)Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10gNaCl 10g Agar 瓊脂() 1-2%Distilled water (蒸餾水) 1000ml pH7.0適用范圍:大腸xx菌(大腸桿
34、菌)34. Penssay Broth (Penssayfe)湯)Beef extract (牛肉膏)1.5g Yeast extract 酵母膏()1.5gTryptone (胰蛋白胨) 5g NaCl3.5gGlucose (葡萄糖) 1g K2HPO4.3H2O4.8gDistilled water (蒸餾水 ) 1000ml pH7.235. Blond Agar (血瓊脂培養(yǎng)基)Peptone (蛋白胨) 10g Glucose 葡萄糖() 2gNaCl 5g NaH2PO42.5gSodium citrate (檸檬酸鈉) 5g Brain infusion (腦浸汁) 800ml
35、Pig heart infusion (豬心浸) 200ml Sleep Blood 羊血() 10%pH36.Note:Preparation of pig brain and pig heart infusion:( 1) Smash up 100g fresh pig hear(t without fat and blood vessel) .Add distilled water up to 1000ml;( 2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;( 3)
36、 Keep the two infusions at 50 for 1 hr , then boil for 15 minutes;( 4) Cool and filtrate through gauze . 注 豬心和豬腦浸汁法:( 1)新鮮豬心除去心頭和脂肪,攪碎100 克加蒸餾水至1000 毫升;( 2)新鮮豬腦除去血管,取200 克加蒸餾水至1000 毫升;( 3) 50 保溫 1 小時(shí),然后煮開15 分鐘;( 4)冷卻后用紗布過濾備用。NaCl 6g MgSO4.7H2O0.1gKH2PO40.5g CaCl20.1gK2HPO42.0g Yeast extract 酵母膏()1.0
37、g(NH4)2SO42.0g Distilled water (蒸餾水) 1000mlFilter paper strip:one strip (濾紙條:一片 ) pH7.07.4Note:Carboxymethyl Cellulose 2% Agar 2%(斜面培養(yǎng)基去濾紙條用 .2%瓊脂及2%羧甲基纖維素鈉CMCNa)37. Clostridium Pasreurianum Synthetic Medium (巴氏梭狀芽胞桿菌合成培養(yǎng) 基)Glucose (葡萄糖)1% MnSO40.001%KH2PO40.05% FeSO40.001%K2HPO40.05% Yeast extract
38、(酵母膏)0.1%MgSO4.7H2O0.02% Peptone (蛋白胨)0.01%NaCl0.001% CaCO30.5%Distille water (蒸餾水) 100% pH7.038. Yeast Extract-Medium Extract AgaE (酵母膏、麥芽膏瓊脂 U )Yeast extract (酵母膏)4g Mall extract (麥芽膏)10gGlucose (葡萄糖)4g Agar (瓊脂)17gDistilled water (蒸儲(chǔ))1000ml pH7.339. Oatemeal Agar (燕麥粉瓊月旨)Oatemeal (infusion)燕麥粉浸液2
39、0g Agar (瓊脂)18%Trace element solution (跡量鹽溶液)1mlDistilled water up to (用蒸餾水補(bǔ)充至1000 毫升) 1000ml pH7.2Note:Trace element solution (跡量鹽溶液):FeSO4.7H2O0.1g MnCl2.4H2O0.1gZnSO4.7H2O0.1g Distilled water (蒸餾水) 100ml40. Calcium Malate Agar (蘋果酸鈣瓊脂)Calium malate (蘋果酸鈣) 10g K2HPO423 / 650.5gNH4Cl0.5g Glycerol (
40、甘油) 10gAgar (瓊脂) 15g Distilled water (蒸餾水) 1000ml41. Thiobacillus Ferrooxidans Medium (氧化亞鐵硫桿菌培養(yǎng)基)(NH4)2SO40.15g KH2PO40.05gKCl0.05g MgSO4.7H2O0.5gCa(NO3)2.4H2O0.01g FeSO4.7H2O 50gDistilled water (蒸餾水 ) 1000ml pH2.042. Thiobacillus Thiooxidans Medium (氧化硫硫桿菌培養(yǎng)基)(NH4)2SO40.3g KH2PO4 3-4g25 / 65CaCl2
41、(anhydrous) 無水0.25g MgSO4.7H2O0.5gFeSO4.7H2O0.001g Distilled water (蒸餾水) 1000mlpH3.5-443. Plasma Substitute Medium (代血漿培養(yǎng)基)Sucrose (蔗糖)12.5-15% Peptone (蛋白胨)0.5%KH2PO40.03% Na2HPO4.12H2O0.14%pH7.07.244. Skim Milk Medium (脫脂牛奶培養(yǎng)基) ATCC 183脫脂牛奶100g去離子水1000ml適用范圍:xx乳桿菌、嗜熱乳酸鏈球菌45. Pea Agar 豌豆( xx)Glucos
42、e (葡萄糖) 1% Peptone (蛋白胨 )0.5%NaCl0.5% Agar (xx)2.0%Pea infusion (counted by NH2)腕豆浸汁(以 NH2 計(jì))12-14 mg / 100pH (before sterilization) 消前 7.27.5Note:Preparetion of pea infusion (豌豆浸汁的制法) :Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to4.34.5 by usingof sulphur
43、ic acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottlemouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37 for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Di
44、stributionandautoclave at 120 for 30 minutes. Store at cool contion . (稱取白豌豆900 克,置于5 公升廣口瓶中,加自來水3 公升,用硫酸調(diào)pH 至4.3-4.5,再加氯仿30毫升(防腐),加膠塞并用紗布扎口(防止沖脫),放37 恒溫室 7-10 天。取出用濾紙過濾,濾液煮沸20 分鐘,以除氯仿,并再次以濾紙過濾,除沉淀。濾液分裝,于120 30 分鐘滅菌。后冷藏備用。)The standard quality of pea infusion as following (豌豆浸汁質(zhì)量標(biāo)準(zhǔn)):Aminonitrogen (氨
45、基酸):160-200 mg / 100mlSoluble phosphoruse (溶磷):400-600 區(qū) g / mlColor (色澤):auratus,transparent (金黃、透明)46. Wheat Bran Medium (麥麩瓊脂)Wheat bran (麥麩)3.6% (NH4)2HPO40.3%K2HPO40.02% MgSO4.7H2O0.01%Agar (xx) 2% pH7.0適用范圍:金霉素鏈霉菌、紅霉素鏈霉菌47. Glucose Yeast Extract Agar!(萄糖、酵母膏瓊脂)Glucose 翁萄糖)10g Yeast extract (孝母
46、膏)10gWater (水 ) 1000ml Agar (瓊脂) 15gpH7.248. MMN Medium (MMN 培養(yǎng)基 )CaCl20.05g MgSO40.15gNaCl0.025g FeCl3 (1%)1.2mlKH2PO40.5g Vitamib B1 硫胺素)100(NH4)2HPO40.25g Wort (12 Be' ) 100mlGlucose (葡萄糖) 10-15g Citric acid 檸檬酸()0.2gDistilled water (蒸餾水) 900ml pH5.5 (左右)適用范圍:蜜環(huán)菌49. Starch Agar (淀粉瓊脂 )Soluble
47、 starch (可溶性淀粉) 10g NaNO3 1gMgCO3 1g K2HPO40.3gNaCl0.5g Agar (xx) 20gDistilled water (蒸餾水) 1000ml50. 5 Be ' Wort Agar (5BI汁瓊月旨)適用范圍:園弧青霉、少根根霉、xx根霉、xx根霉51. 5 Be ' Koji Extract Agar 傣Be十瓊脂)適用范圍:華根霉、三寶壟根霉52. 發(fā)根農(nóng)桿菌培養(yǎng)基(YEB)Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏 ) 1g/LPeptone (蛋白胨) 5g/L Sucro
48、se 蔗糖() 5g/LMgSO31 / 654.7H2O0.4g/100ml Agar (瓊脂)1.5g/100mlpH7.4適用范圍:青枯假單胞菌53. 青枯菌培養(yǎng)基(Tm)Peptone (蛋白月東)10g Casein干酪素水解物產(chǎn))1gGlucose (葡萄糖) 5g Ddhro (純水 )1000mlAgar(xx) 15g pH7.054. YM (Yeast Malt Agar)酵母膏3.0gxx 汁3.0g蛋白胨5.0gxx20.0g蒸餾水 1000ml55. ISP 4# / 65可溶性淀粉10.0g K2HPO41.0gMgSO4.7H2O1.0g NaCl1.0g(NH
49、4)2SO42.0g CaCO32.0gFeSO4.7H2O0.001g MnCl2.7H2O0.001gxx20.0g 蒸儲(chǔ)水 1000ml56. Trypton Soy Agar 胰蛋白胨17.0g大豆月東3.0g葡萄糖2.5gxx20.0gNaCl5.0g K2HPO42.5g蒸餾水 1000ml57. NA (Nutrient Agar)牛肉膏3.0g蛋白月東5.0gxx20.0g 蒸儲(chǔ)水 1000mlpH6.67.058. 甘露(糖 )醇 xx(ATCC 1)酵母膏5.0g蛋白月東3.0g甘露(糖 )醇33 / 650.008g15.0g蒸餾水 1000ml59. Marine 瓊脂
50、 2216 (ATCC 2)蛋白胨5.0g酵母膏1.0g檸檬酸鐵0.1g澳化鉀0.08gNaCl19.45g MgCl28.8gNa2SO43.24g CaCl21.8gKCl0.55g NaHCO30.16gNH4NO30.0016g Na2HPO4氯化鍶0.034g硼酸0.022g硅酸鈉0.004g氟化鈉0.0024gxx15.0g 蒸儲(chǔ)水 1000mlpH7.47.860. 兔血xx(ATCC 4)牛心(取其浸液)500.0g 胰蛋白月東(Tryptose)10.0gNaCl5.0gxx15.0g蒸餾水 1000ml pH6.6-# / 657.061. 孢子形成培養(yǎng)基(ATCC 5)酵
51、母膏1.0g 牛肉膏1.0g胰蛋白胨2.0g葡萄糖10.0gFeSO4散量 xx15.0g蒸餾水 1000ml pH7.262. Trypticase Soy Agar (ATCC 18)Trypticase Soy Broth30.0gxx15.0g蒸餾水 1000ml63. YPAD (ATCC 109)酵母膏10.0g蛋白月東20.0g37 / 65葡萄糖20.0gxx20.0g蒸餾水 1000ml pH7.064. SP (ATCC 432)棉子糖1.0g蔗糖1.0g半乳糖 1g 可溶性淀粉5g水解酪蛋白2.5gxx20.0gMgSO4.7H2O0.5g K2HPO40.25g蒸餾水
52、1000ml pH 自然65. 胰酪胨大豆酵母浸膏瓊脂(TSA-YE)胰蛋白胨15.5g大豆蛋白月東41 / 65NaCl5.0g酵母膏6.5gxx15.0g 蒸儲(chǔ)水 1000mlpH7.366. ATCC 20蛋白胨5.0g酵母膏15.0gK2HPO43.0g葡萄糖2.0gxx20.0g 蒸儲(chǔ)水 1000mlpH7.37.567. TY (Yeast Tryptone Agar)蛋白胨10.0g酵母膏5.0g葡萄糖1.0g NaCl5.0gxx20.0g 蒸儲(chǔ)水 1000mlpH7.068. 硫醇(Thiol)培養(yǎng)基(ATCC 49)示蛋白胨(Proteose Peptone No.3,Di
53、fco)10.0g酵母膏5.0g葡萄糖1.0g NaCl5.0g硫醇復(fù)合物8.0gxx1.0gp-氨基苯甲酸2.5g6.97.369. CzapeK Dox 瓊脂培養(yǎng)基(ATCC 134)Czapek Dox Broth35.0gxx15.0g蒸餾水 1000ml70. MG/L蛋白胨5.0gxx5.0g谷氨酸鈉1.15g 生物素0.0001gK2HPO40.25g NaCl0.1gMgSO4.7H2Oxx20.0g 蒸儲(chǔ)水 1000ml氯霉素(終濃度)50止g/mlpH7.071. Eclb Broth蛋白胨10.0g酵母膏5.0gNaCl5.0gxx20.0g蒸儲(chǔ)水1000ml氨節(jié)青霉素(終濃度)300 wg/mlpH7.0酵母膏1.0g 牛肉膏1.0gNZ Amine,type A2.0g葡萄糖43 / 6517.5gxx20.0g 蒸儲(chǔ)水 1000mlpH7.373. Harrold /
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