常見載體引物與序列

1、In vitroge n中國使用的測(cè)序載體和引物Primer of VectorVectorPrimer(F)Primer(R)PACTT7T3pACT2GAL4 ADpACT2-RpAS2-1GAL4 BDpAS2-1.RpB42ADpB42ADFpB42ADRpBACPAK8BAC1BAC2pBK-CMVT7T3PBS(SK/KS)/M13-:M13F/T7T3/M13RpBV220PBV220FPBV220RpCAMBIA 1301(1300)P1P2pCAMBIA 2300M13R(-48)M13F(-47)PCANTB5ES1/M13RS6pCAT3-e nhancerRVP3此載體

2、無反向引物pcDNA3.0CMV-F/T7SP6/BGHpcDNA3.1T7BGHpcDNA4T7/CMV-FBGHpcDNA6T7/CMV-F(J21025在 T7前面)BGHpcDNAIIT7SP6pCE2.1M13FM13RpCEP4pCEP-FEBV-RpCF-TM13FM13RpCIT7 (17Base)此載體無反向引物pCI-neoT7 (17Base)T3pCMS-EGFPT7T3pCMV-3Tag-4AT3 /PFLAG-CMV-FT7pCMV5pCMV5FpCMV5RpCMV5-FlagpCMV5FpCMV5RpCMV-MYC/HA:pCMV-FpCMV-RpCMV-Spor

3、tM13F/T7SP6/M13RpCMV-Tag(KAN+):T3T7pCR2.1-TOPOM13F/T7M13RpCR3.1T7BGHpCS2SP6T7pDonarM13FM13RpDONR221M13FM13RpDriveT7SP6pDRIveR(KAN+)T7SP6pDsRED1-C1:pDsRED-ex-C1-FpEGFP-N-3 （距離很近,一般不用）pDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3pDSRED-N1(KAN+):pEGFP-N-5PDSRED-N-RpECFP-CpEGFP-C-5pECFP-C-3'pECFP-N1pEGFP-

4、N-5pEGFP-N-3pEF/mvc/ERpEF-FpCDNA3.1RpEGFP-C(KAN+)pEGFP-C-5pEGFP-C-3'pEGFP-N(KAN+)pEGFP-N-5pEGFP-N-3pENTR/D-TOPO(KAN-MI13FM13RpET-*(His)(KAN+)T7T7 TerpFT22(a b c)(AMP+)T7T7 TerpFT28 30 24,49(KAN+T7T7 TerpET32(a b c)(AMP+)T7/S.tagT7 TerpET50(amp+)T7/s.tagT7TERpET44(AMP+)T7/s.tagT7 TerpEYFP-N1pEGFP

5、-N-5pEGFP-N-3pEYFP-C1pEGFP-C-5pEGFP-C-3'pFLAG-CMVpFLAG-CMV -FpFLAG-CMV -RpGAD424GAL4 AD3'ADpGADGHGAL4 AD3'ADpGADT7-RecGAL4 AD/T73'ADpGAPZa-Aa-FACTOR3'AOXpGBKT7(Ka na+)T73'BDpGEM-T(-Easy)M13F/T7SP6/M13RpGEX-(*)T:pGEX-4T-5'pGEX-4T-3pGL2GLP1GLP2pGL3RVP3GLP2(RVP4)沒有特別說明用GLP2)

6、Pin poi ntTM VectorPinpoint PrimerSP6pIRESPIRES-F/T7T3/PIRES-R (J40720)plRES2-EGFP1pIRES2-EGFP.P5pIRES2-EGFP.P3pLXSNpLXSN-FpLXSN-RpMAL-c2EMalE primerM13F(-47)pMAL-C2xP5'P3'pMAL-p2XMalE primerM13F(-47)PMD18-TM13R(-48)M13F(-47)PMD19-TM13F(-47)M13R(-48)pPIC9K(AMP+ ZEO+ 5 ' AOX/a-factor3 

7、9; AOXpPICZa5'AOX/PPICZa-F3'AOXpPROEXHTAM13R(-48)無反向引物pQE30or40pQE30-FpQE-R:pRSETT7T7TERpSile ncer3.1-H1 hygroM13F(-47)3.0REVpSILENCEV2.0-U6T72.0REVpSK01-TM13FM13RpSK-CMV(KAN+)T7T3pSP72T7SP6pSport1SP6/M13F(-47)T7/M13RpSUPERT7T3pTA2M13F/T7(優(yōu)先使用M13FT3/M13RpT-AdvM13F /T7M13RpTARGET TMpTarget.F(

8、在T7前面)/T7pTarget.RpTO-T7T7T7TERpTRC99a-cpTRC99C-FpTRC99C-RpTriPLEx25'pTriPLEx2T7pTWIN-1T7T7TER客戶確認(rèn)再用)pUC18(19)/118(119):M13F(-47)M13R(-48)pUCm-TM13F/T7M13RpVAX1T7BGHpWSK29T7/M13F/M13F(-47)T3/M13R/M13R(-48)pXT7T7SP6pYES2T7pYes2.RpLEXApLEXA-FpLEXA-RpLNCXpLNCX-FpLNCX-RpRECEIVER1CMV-F此載體無反向引物pSHUTTL

9、E-CMVPSHUTTLE-CMV-FPSHUTTLE-CMV-RpSP64/65SP6此載體無反向引物pSTBlue-1T7SP6pT7Blue(R)T7M13F(-47)In vitroge n中國測(cè)序通用引物序列引物名稱序列(5'-3')M13RCAG GAA ACA GCT ATG ACCM13FTGT AAA ACG ACG GCC AGTM13F(-47)CGC CAG GGT TTT CCC AGT CAC GACM13R(-48)AGC GGA TAA CAA TTT CAC ACA GGAM13(-96)CCC TCA TAG TTA GCG TAA CGSP

10、6ATT TAG GTG ACA CTA TAGT7TAA TAC GAC TCA CTA TAG GGT7 termi natorJgC TAG TTA TTG CTC AGC GGT3ATT AAC CCT CAC TAA AGG GApGEX-4T-5'GGG CTG GCA AGC CAC GTT TGG TGpGEX-4T-3'CCG GGA GCT GCA TGT GTC AGA GGGLp1TGT ATC TTA TGG TAC TGT AAC TGGLp2CTT TAT GTT TTT GGC GTC TTC CARVp3CTA GCA AAA TAG GCT

11、GTC CCRVp4GAC GAT AGT CAT GCC CCG CGpcDNA3.1RTAG AAG GCA CAG TCG AGGPinPoint primerCGT GAC GCG GTG CAG GGC GpCMV-FTCT AAA AGC TGC GGA ATT GTpCMV-RTCC AAA CTC ATC AAT GTA TCPTRC99C-F:TTG CGC CGA CAT CAT AACPTRC99C-R:CTG CGT TCT GAT TTA ATC TGpCEP-F:AGA GCT CGT TTA GTG AAC CGEBV-R :GTG GTT TGT CCA AAC

12、 TCA TCplRES2-EGFP.P5 :GTA GGC GTG TAC GGT GGG AGplRES2-EGFP.P3 :AAC GCA CAC CGG CCT TAT TC3'AD:AGA TGG TGC ACG ATG CAC AGCMV -FCGC AAA TGG GCG GTA GGC GTGS1CAA CGT GAA AAA ATT ATT ATT CGCS6GTA AAT GAA TTT TCT GTA GTA GG5、A0X1GAC TGG TTC CAA TTG ACA AGC3、A0X1GCA AAT GGC ATT CTG ACA TCCa -FactorT

13、AC TAT TGC CAG CAT TGC TGCGAL4 ADTAC CAC TAC AAT GGA TGpACT2-RGTG CAC GAT GCA CAG TTG AApB42ADF:CCA GCC TCT TGC TGA GTG GAG ATGpB42ADR:AAG CCG ACA ACC TTG ATT GGA GpEGFP-N-5 :TGG GAG GTC TAT ATA AGC AGA GpEGFP-N-3 :CGT CGC CGT CCA GCT CGA CCA GpEGFP-C-5 :CAT GGT CCT GCT GGA GTT CGT GpEGFP-C-3':T

14、AT GGC TGA TTA TGA TCA GTPBV220F:AAG AAG GGC AGC ATT CAA AGPBV220R:CTG CGT TCT GAT TTA ATC TGU6ATG GAC TAT CAT ATG CTT ACC GTA2.0rev primer:AGG CGA TTA AGT TGG GTA3.0rev:GAG TTA GCT CAC TCA TTA GGCS.tagGAA CGC CAG CAC ATG GAC5'TriplF x2CTC CGA GAT CTG GAC GAG CRVP4GAC GAT AGT CAT GCC CCG CGRVP3C

15、TA GCA AAA TAG GCT GTC CCpQE30-RGTT CTG AGG TCA TTA CTG GpQE30-F(TGA GCG GAT AAC AAT TTC ACpEF-FTCA AGC CTC AGA CAG TGG TTCpDSRED-N1-RTGA AGC GCA TGA ACT CCT TGpDsRED-express-C1-FTCC CAC AAC GAG GAC TAC ACpCMV5RATT ATA GAG GAC ACC TAG TCpCMV5FfrTC CAA AAT GTC GTA ATA ACP5'TGC GTA CTG CGG TGA TCA

16、 ACP3'CTG CAA GGC GAT TAA GTT GGBAC2ACG CAC AGA ATC TAG CGC TTBAC1AAC CAT CTC GCA AAT AAA TA3'BD:frAA GAG TCA CTT TAA AAT TTG TAT ACpTarget.FCCA GGA TTT TCC CAG TCA CpTarget.RGGC TTT ACA CTT TAT GCT TCT7(17base)ACA TCC ACT TTG CCT TTC TCpYes2.RfrCG GTT AGA GCG GAT GTGGAL4 BDTCA TCG GAA GAG A

17、GT AGpAS2-1.RAAG AGT TAC TCA AGA ACA AGA ApFlag-CMV-F:GGT AGG CGT GTA CGG TGGpFlag-CMV-R:GCA CTG GAG TGG CAA CTTpIRES-F:CTT ACT GAC ATC CAC TTT GCpIRES.R:CAC TGC ATT CTA GTT GTG GTP1CCA GGC TTT ACA CTT TAT GCP2GCG ATT AAG TTG GGT AAC GCpLEXA-FCGT CAG CAG AGC TTC ACC ATpLEXA-RTAA AAC CTA AGA GTC ACT TTpLNCX-