苦碟子中的新黃酮苷

1、    苦碟子中的新黃酮苷        關(guān)鍵詞苦碟子；黃酮苷；木犀草素-7-O-D-吡喃葡萄糖醛酸苷甲酯；芹菜素-7-O-D-吡喃葡萄糖醛酸苷甲酯New Flavonoids from Ixerissonchifolia HanceFeng Xizhi,Xu Suixu,Yue Dabiao,Feng WenjunDepartment of Natural Medicinal Chemistry,Shenyang Pharmaceutical University,Shen

2、yang 110015AbstractTwo new flavonoids,named luteolin-7-O-D-glucuronide methyl ester and apigenin-7-O-D-glucuronide methyl ester,were isolated from the whole plant of Ixeris sonchifolia(Bge.)Hance.Their structures were deduced and confirmed by the chemical method and spectoscopic analysis(1H-NMR,13C-

3、NMR).Key wordsIxeris sonchifolia(Bge.)Hance;flavonoids;luteolin-7-O-D-glucuronide methyl ester;apigenin-7-O-D-glucuronide methyl ester苦碟子為菊科植物抱莖苦荬菜Ixeris sonchifolia(Bge.)Hance的當年生干燥全草.具有清熱解毒、排膿之功效.利用大孔樹脂、硅膠柱層析及聚酰胺柱層析從中分離得到兩個新的黃酮苷類成分，其中一個命名為木犀草素-7-O-D-吡喃葡萄糖醛酸苷甲酯(luteolin-7-O-D-glucuronide methyl est

4、er,)，另一個為芹菜素-7-O-D-吡喃葡萄糖醛酸苷甲酯(apigenin-7-O-D-glucuronide methyl ester,).這兩種成分是首次從本屬植物中得到.化合物()為黃色針狀結(jié)晶，鹽酸-鎂粉反應(yīng)陽性，Molish反應(yīng)陽性，酸水解檢出葡萄糖醛酸.通過各種譜學的分析13(1H-NMR,13C-NMR)并與已知物木犀草素-7-O-D-吡喃葡萄糖醛酸苷相對照確定了其結(jié)構(gòu).其氫譜、碳譜數(shù)據(jù)見表1.Tab.1The NMR data of compound()(in DMSO-d6)No.13C-NMR 1H-NMR No.13C-NMR 1H-NMR 2164.43145.731

5、03.06.73(s)4150.04181.85115.96.89(d,J=8.1 Hz)5156.86121.27.43(d,J=8.1 Hz)699.36.43(d,J=1.5 Hz)7-glcuA7162.7-199.45.17(d,J=6.0 Hz)894.36.79(d,J=1.5 Hz)-272.89161.0-376.010105.3-471.51119.0-574.52113.47.40(br s)-6170.88-OCH351.9化合物()為淡黃色顆粒狀結(jié)晶，鹽酸-鎂粉反應(yīng)陽性，Molish反應(yīng)陽性.通過各種譜學分析13(1H-NMR，13C-NMR)并與已知物芹菜素-7-O

6、-D-吡喃葡萄糖醛酸苷比較確定了其結(jié)構(gòu).其氫譜、碳譜數(shù)據(jù)見表2. Tab.2The NMR data of compound()(in DMSO-d6)No.13C-NMR 1H-NMR No.13C-NMR 1H-NMR 2165.43117.26.93(d,J=8.7 Hz)3104.46.65(s)4162.84183.25117.26.93(d,J=8.7 Hz)5158.26129.97.88(d,J=8.7 Hz)6100.66.47(d,J=1.2 Hz)7-glcuA7163.6-1100.35.19(d,J=6.0 Hz)895.96.79(d,J=1.5 Hz)-273.9

7、9162.4-376.610106.7-472.51122.3-576.42129.97.88(d,J=8.7 Hz)-6170.4-OCH351.92哈爾濱中藥二廠科研所，哈爾濱 1500783哈爾濱中藥二廠，哈爾濱 150078作者單位：沈陽藥科大學天然藥物化學教研室，沈陽 110015 參考文獻1.Harborne JB,Christine A Willians.Flavonoid patterns in the fruits of the Umbelliferae. Phytochemistry,1972，11(5)：174117502.Francisco AT,Harborne JB,Ron S.Twelve 6-oxygenated flavone sulfates from Lippianadiflora and L.can