細(xì)胞流體血流剪切應(yīng)力平行平板流動(dòng)室 flow chamber

1、Parallel Plate Flow Chambers Human Neutrophils Rolling on Human Endothelial Cells Expressing E-selectinParallel plate flow chambers are used in dynamic studies of cell adhesion under well-defined shear forces. Various gasket sizes allow flexibility in experimental design to operate with li

2、mited quantities of cells and/or reagents. Circular Flow Chamber (for 35mm tissue culture dishes) 31-001Circular Flow Chamber Kit     Flow Chamber Kit Includes:Two flow decks   (cast acrylic with different inlet/outlet designs)8 silicon rubber gasket

3、s (two of each size)Flow Width = 0.25 cm, Thickness = 0.005 inFlow Width = 0.25 cm, Thickness = 0.010 inFlow Width = 0.50 cm, Thickness = 0.010 inFlow Width = 1.00 cm, Thickness = 0.010 in24 polypropylene tubing connectors(six of each) Threaded 1/16 in. straight and L-connectorsMale and Fe

4、male taper luer to 1/16 in. connectorsSilastic? laboratory tubing (1/16 in. ID x 10 ft.)  平行板流動(dòng)培養(yǎng)艙(Parallel-Plate Flow Chamber)是體外研究細(xì)胞力學(xué)最常用的模型之一，用來(lái)模擬體內(nèi)各種細(xì)胞處于生理環(huán)境中，暴露在動(dòng)態(tài)液體流作用下所受到的流體剪剪應(yīng)力。細(xì)胞的代謝反應(yīng)與所受到的壁面剪切應(yīng)力密切相關(guān)。MICFORCE米力光CO.LTD典型的平行板流動(dòng)培養(yǎng)艙由三個(gè)部分組成：聚碳酸酯分液室，硅膠墊圈和玻璃蓋玻片。分液室構(gòu)成流動(dòng)培養(yǎng)艙的一側(cè)，包括進(jìn)液口，出液

5、口和真空槽。墊圈的厚度決定流程長(zhǎng)度。蓋玻片構(gòu)成流動(dòng)小室的另一側(cè)，其上覆蓋細(xì)胞外基質(zhì)蛋白(ECM)，血管細(xì)胞或其他待研究的生物材料。真空形成一個(gè)密封環(huán)境將三個(gè)部件緊密連接在一起，同時(shí)也確保通道高度的一致性。1.平行板流動(dòng)培養(yǎng)艙能夠產(chǎn)生生理學(xué)范疇內(nèi)的壁面剪應(yīng)力：0.01-30 dyn/cm2。通過(guò)微量注射泵以可控的液體流量(如抗凝的全血或者細(xì)胞懸液)注入小室并流進(jìn)固定化基質(zhì)的方法產(chǎn)生剪切應(yīng)力。2.裝置設(shè)計(jì)，組裝和操作簡(jiǎn)單，方便；3.可以在設(shè)定的時(shí)間-周期內(nèi)研究穩(wěn)定剪應(yīng)力對(duì)細(xì)胞的影響；也可以隨實(shí)驗(yàn)需求調(diào)節(jié)流速和剪切應(yīng)力；細(xì)胞可在流動(dòng)液體條件下生長(zhǎng)，能夠在顯微鏡下隨時(shí)觀察生長(zhǎng)狀態(tài)；或者利用視頻顯微鏡實(shí)

6、時(shí)監(jiān)控細(xì)胞狀態(tài)；研究方向：1.模擬體內(nèi)血液流動(dòng)產(chǎn)生的血流剪切切力，研究不同dyn/cm2層流脈動(dòng)剪切應(yīng)力下成骨細(xì)胞破骨細(xì)胞軟骨細(xì)胞血管平滑肌細(xì)胞血管內(nèi)皮細(xì)胞骨髓充間質(zhì)干細(xì)胞和內(nèi)皮細(xì)胞之間的動(dòng)態(tài)粘附作用或細(xì)胞間的相互作用等；2.利用平行板流動(dòng)培養(yǎng)艙產(chǎn)生剪應(yīng)力模擬腫瘤細(xì)胞的體內(nèi)生長(zhǎng)環(huán)境，從而研究腫瘤細(xì)胞的增殖，吸附和轉(zhuǎn)移機(jī)制。3.細(xì)胞趨化實(shí)驗(yàn)中作為藥物測(cè)試體系，基于細(xì)胞-內(nèi)皮細(xì)胞粘附過(guò)程作為新藥物的運(yùn)輸體系； Glycotech公司提供兩種平行板流動(dòng)培養(yǎng)艙：Rectangular Flow Chamber(矩形流動(dòng)培養(yǎng)艙)和Circular Flow Chamber(圓形流動(dòng)艙)，含有平

7、行流動(dòng)小室裝置需要的基本組成部件。客戶(hù)根據(jù)自身實(shí)驗(yàn)需求選擇合適的產(chǎn)品。(1).Rectangular Flow Chamber(長(zhǎng)方形形流動(dòng)培養(yǎng)艙可以用于標(biāo)準(zhǔn)顯微載玻片)Rectangular Flow Chamber Kit包含附件： 1個(gè) 流動(dòng)板 (灌注的聚丙烯酸材質(zhì))4個(gè) 硅橡膠墊圈(每種尺寸各2個(gè))Flow Width = 1.00 cm, Thickness = 0.005 inFlow Width = 1.00 cm, Thickness = 0.010 in24個(gè) 聚丙烯管接頭(每種尺寸各6個(gè))Threaded 1/16 in. straight a

8、nd L-connectorsMale and Female taper luer to 1/16 in. connectors硅橡膠實(shí)驗(yàn)軟管(1/16 in. ID x 10 ft.) Rectangular Flow Chamber (for standard microscope slide)31-010Rectangular Flow Chamber Kit   Flow Chamber Kit Includes:One flow deck (cast acrylic)4 silicon rubber gaskets (two of each size)Flow Width = 1.00 cm, Thickness = 0.005 inFlow Width = 1.00 cm, Thickness = 0.010 in24 polypropylene tubing connectors(six of each) Threaded 1/16 in.