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1、HomeTechnicalReferenceRestrictionEndonucleasesNEBufferActivityChartQ_5HRREU匕回NEBufferActivityChartforRestrictionEnzymesForyourconvenience,NewEnglandBiolabsoffersasimple4buffersystem.Acolor-coded10XNEBufferissuppliedwitheveryreendonuclease,ensuring100%activity.Over170restrictionenzymesexhibit100%acti

2、vityinNEBuffer4,resultinginincreasedeffandeaseofuseespeciallywhenperformingdoubledigests.Tohelpselectthebestconditionsfordoubledigests,thischartshowstheoptimal(supplied)NEBufferandapproximateactivityintNEBuffersandNEBufferEcoRIforeachenzyme.IfBSAissuppliedwithanenzyme,itisincludedinallNEBufferactivi

3、tyreactions.tableshowsrecommendedreactiontemperature,heatinactivationtemperature,recommendeddiluentbufferandwhethertheenzymqualified(i.e.,cleavessubstratein5minutesunderrecommendedconditions).Note:ThevalueslistedinthistableareapproximaobtainedusingeachenzymesspecificunitassaysubstrateDNA.Pleasecheck

4、outothertechnicalreferenceinformationrelatedtorestrictionenzymes:DoubleDigestion|HeatInactivation|Activityat37C|DiluentBuffers|Time-SaverEnzymes|HighFidelity(HF)RestrictionEnzClickfortheChartLegend,IconDescriptionA|B|C|D|E|F|H|I|K|L|M|N|P|R|S|T|X|Z|EnzymeSuppliedNEBuffer%ActivityinNEBuffersHeatInac.

5、CIncu.Temp.CDiluent1234EcoRIAatllNEBuffer405050100256537AAcc65INEBuffer3+BSA107510025506537AAccINEBuffer450501010058037AAcilNEBuffer32550100501006537AAcllNEBuffer4+BSA101001005No37BAcuINEBuffer4+SAM50100501001006537BAfelNEBuffer42550251001006537BAflllNEBuffer4+BSA501002510006537AAfllllNEBuffer3+BSA2

6、575100501008037BAgelNEBuffer1100501075506537CAgel-HFNEBuffer4+BSA1005010100256537AAgel-HFRE-Mix-NRNRNRNR-6537-AhdINEBuffer4+BSA25750100106537AAlelNEBuffer410251010006537AAlulNEBuffer4100100751001006537BAlwlNEBuffer4501001010056537AAlwNINEBuffer410100100100106537AApalNEBuffer4+BSA2550010056525AApaLIN

7、EBuffer4+BSA100100101005No37AApeKINEBuffer3257510050100No75BApoINEBuffer3+BSA1075100751008050AAsciNEBuffer40101010056537AAsciRE-Mix-NRNRNRNR-6537-AseINEBuffer3NR75100ddNR506537BAsiSINEBuffer4+BSA5010010010008037BAvalNEBuffer4107510100508037AAvailNEBuffer4507510100506537AAvrIINEBuffer4100100501005080

8、37BEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentBaeGINEBuffer11007510501006537ABaeINEBuffer4+BSA+SAM5010050100406525ABamHINEBuffer3+BSA75100100dd100100No37ABamHI-HFNEBuffer4100501010025No37ABanINEBuffer4+BSA5010050100256537ABanIINEBuffer410010501001008037ABbsINEBuffer2100100257550

9、6537BBbvCINEBuffer450100101001008037BBbvINEBuffer210010025751006537BBccINEBuffer1+BSA100501050156537ABceAINEBuffer3+BSA1001001001001006537ABcgINEBuffer3+SAMNRNR100ddNR506537ABciVINEBuffer4100500100256537CBclINEBuffer350100100dd75100No50ABcoDINEBuffer45075751001006537BBfaINEBuffer475501010008037BBfuA

10、INEBuffer3075100101006550BBfuCINEBuffer4+BSA1005025100158037BBgllNEBuffer35075100501006537BBglllNEBuffer3107510010100No37ABlpINEBuffer4501001010050No37ABmgBINEBuffer3+BSA02510010506537CBmrINEBuffer27510075100506537BBmtlNEBuffer22510025501006537BBmtI-HFNEBuffer4+BSA5010010100-6537BBpmlNEBuffer3+BSA75

11、1001001001006537BBpulOINEBuffer31025100251008037BBpuEINEBuffer4+SAM50100101001006537BBsaAINEBuffer4100100100100100No37CBsaBINEBuffer450100751001008060BBsaHINEBuffer4+BSA501001001001008037ABsalNEBuffer4+BSA75751001001006537BBsal-HFNEBuffer4+BSA252510100106537BBsaJINEBuffer41001001001001008060ABsaWINE

12、Buffer4+BSA5010050100408060ABsaXINEBuffer4751001010015No37BBseRINEBuffer4100100751001006537ABseYINEBuffer31050100501006537BBsglNEBuffer4+SAM507550100206537BBsiEINEBuffer4+BSA501001010058060ABsiHKAINEBuffer4+BSA5010010010025No65BBsiWINEBuffer3100100100251008055ABsllNEBuffer31050100751008055ABsmAINEBu

13、ffer4501001001001008055BBsmBINEBuffer3751001001001008055BBsmFINEBuffer4+BSA255050100158065ABsmINEBuffer4751007510058065ABsoBINEBuffer21010010050100No37ABsp1286INEBuffer4+BSA252525100106537ABspCNINEBuffer4+BSA+SAM1007510100158025ABspDINEBuffer42575501001006537ABspEINEBuffer301010001008037BBspHINEBuff

14、er4101005010056537ABspMINEBuffer3NRNR100NR1006537BBspQINEBuffer410501001001008050BBsrBINEBuffer4501001001001008037ABsrDINEBuffer2+BSA501005075508065ABsrFINEBuffer410100100100100No37CBsrGINEBuffer2+BSA2510010100508037ABsrINEBuffer3050100101008065BBssHIINEBuffer3100100100dd1001008050BBssKINEBuffer3+BS

15、A050100501008060ABssSINEBuffer3+BSA050100dd101008037BBstAPINEBuffer4+BSA251001001001008060ABstBINEBuffer475502510050No65ABstEIINEBuffer3+BSA507510075100No60ABstEII-HFNEBuffer4+BSA5075100100-No37ABstEII-HFRE-Mix-NRNRNRNR-No37-BstNINEBuffer2+BSA101001007550No60ABstUINEBuffer410010050100100No60ABstXINE

16、Buffer3050100251006537BBstYINEBuffer25010075100508060ABstZ17INEBuffer4NRNR100100100No37BBsu36INEBuffer3+BSA0251000158037ABtglNEBuffer3+BSA25501001001008037BBtgZINEBuffer4+BSA10250100508060ABtsCINEBuffer4501005010025No50BBtsINEBuffer4+BSA1005050100258055ABtsIMutlNEBuffer4+BSA1005010100-8055AEnzymetop

17、SuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentCac8INEBuffer45075100100506537BClalNEBuffer4+BSA105050100506537ACspCINEBuffer4+SAM1010010100206537ACviAIINEBuffer4+BSA752510100256525CCviKI-1NEBuffer41050251001008037ACviQINEBuffer3+BSA75100100750No25BEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatIn

18、cu.Dilu-Inac.Temp.entDdelNEBuffer375100100751006537ADpnlNEBuffer4100100751001008037BDpnIINEBufferDpnIINRNR100NR1006537BDralNEBuffer47575501001006537ADralllNEBuffer3+BSA1007510025506537BDralll-HFNEBuffer40501010050No37BDrdINEBuffer4+BSA2550101001006537AEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.I

19、ncu.Temp.DiluentEaelNEBuffer4100100501001008037AEagINEBuffer31025100101006537CEagl-HFNEBuffer4251001001001006537BEarlNEBuffer4100100501001006537AEcilNEBuffer41005050100-6537AEco53klNEBuffer41005025100106537AEcoNINEBuffer4100100751001006537AECOO109INEBuffer4+BSA10010075100156537AECOP15INEBuffer3+BSA+

20、ATP75100100100506537AEcoRINEBufferEcoRI1001001001001006537CEcoRI-HFRE-Mix-NRNRNRNR-6537-EcoRI-HFNEBuffer410100010056537CEcoRVNEBuffer3+BSA507510050508037AEcoRV-HFRE-Mix-NRNRNRNR-6537-EcoRV-HFNEBuffer4251001001001006537BEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentFatINEBuffer21010

21、050501006555AFauINEBuffer41005010100506555AFnu4HINEBuffer4102525100106537AFokINEBuffer410010075100256537AFseINEBuffer4+BSA100750100506537BFspEINEBuffer4+BSANRNRNR100-6537BFspINEBuffer4107510100506537CEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatIncu.Dilu-Inac.Temp.entHaellNEBuffer4+BSA7510050100258037A

22、HaelllNEBuffer450100251001008037AHgalNEBuffer11007550100256537AHhalNEBuffer4+BSA751001001001006537AHincIINEBuffer3+BSA751001001001006537BHindlllNEBuffer2501001050256537BHindlll-HFNEBuffer41010010100108037BHinflNEBuffer475100751001008037AHinPIINEBuffer41001001001001006537AHpalNEBuffer4252510100100No3

23、7AHpallNEBuffer1100501050156537AHphINEBuffer4NR750100106537BHpy166IINEBuffer410010050100206537CHpy188INEBuffer4502510100506537AHpy188IIINEBuffer4+BSA1001001010056537AHpy99INEBuffer4+BSA10100100256537AHpyAVNEBuffer4+BSA10010025100506537BHpyCH4IIINEBuffer41005025100208037AHpyCH4IVNEBuffer1100251025156

24、537AHpyCH4VNEBuffer4505025100506537BEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentI-CeuINEBuffer4+BSA10100100-6537BI-ScelNEBufferI-SceI+BSA10505050-6537BEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentKasINEBuffer4+BSA2510001001006537BKpnINEBuffer1+BSA1007505025No37

25、AKpnI-HFNEBuffer41002501000No37AKpnI-HFRE-Mix-NRNRNRNR-No37-EnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentLpnPINEBuffer4+BSANRNRNR100-6537BEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatIncu.Dilu-Inac.Temp.entMbolNEBuffer4751001001001006537AMboIINEBuffer4100100501001006537CMfelNEBuffer4

26、75501010056537AMfel-HFRE-Mix-NRNRNRNR-6537-Mfel-HFNEBuffer475501010006537AMIuCINEBuffer4100101010008037AMlulNEBuffer32575100501006537AMlylNEBuffer4+BSA505025100256537AMmelNEBuffer4+SAMNRNRNR100dd508037BMnIINEBuffer4+BSA7510050100506537BMscINEBuffer47575751001006537BMselNEBuffer4+BSA7510075100506537A

27、MslINEBuffer410010025100106537AMspAIINEBuffer4+BSA5010050100506537BMspINEBuffer4751005010050No37AMspJINEBuffer4+BSANRNRNR100-6537BMwoINEBuffer3107510075100No60BEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentNaelNEBuffer41007510100256537ANarlNEBuffer41007575100506537ANb.BbvCINEBuffer

28、25010010100-8037ANb.BsmINEBuffer32510010025-8065ANb.BsrDINEBuffer2101002550-8065ANb.BtsINEBuffer4+BSA7510075100-8037ANciINEBuffer4100251010025No37ANcoINEBuffer31001001001001006537ANcoI-HFRE-Mix-NRNRNRNR-8037-NcoI-HFNEBuffer450100101001008037BNdeINEBuffer475100751001006537ANgoMIVNEBuffer4100501010008

29、037ANheINEBuffer2+BSA10010010100156537CNheI-HFRE-Mix-NRNRNRNR-8037-NheI-HFNEBuffer4+BSA10010010008037CNlalllNEBuffer4+BSA25252510056537BNIaIVNEBuffer4+BSA1010101001006537BNmeAIIINEBuffer4+SAM1010010008037BNotlNEBuffer3+BSA050100251006537CNotl-HFRE-Mix-NRNRNRNR-6537-NotI-HFNEBuffer4+BSA25100251005065

30、37ANruINEBuffer3010100101006537ANsilNEBuffer31075100251008037ANspINEBuffer2+BSA100100010056537ANt.AIwINEBuffer2251002550-8037ANt.BbvCINEBuffer45010010100-8037ANt.BsmAINEBuffer41005010100-6537ANt.BspQINEBuffer3107510025-8050BNt.BstNBINEBuffer301010010-8055ANt.CviPIINEBuffer42510050100-6537AEnzymetopS

31、uppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentPacINEBuffer1+BSA100751010056537APacIRE-Mix-NRNRNRNR-6537-PaeR7INEBuffer4251001010050No37APciINEBuffer3+BSA50751005008037BPflFINEBuffer4+BSA0252510006537APflMINEBuffer3+BSA0100100501006537APhoINEBuffer3505010075100No75API-PspINEBufferPI-PspI+BSA0

32、101010-No65BPI-SceINEBufferPI-SceI+BSA0000-6537BPleINEBuffer410100100100506537APmeINEBuffer4+BSA0501010056537APmlINEBuffer1+BSA10075075156537APpuMINEBuffer4025010010No37BPshAINEBuffer4+BSA5050010056537APsiINEBuffer41010010100106537BPspGINEBuffer47510050100100No75APspOMINEBuffer4252510100156537BPspXI

33、NEBuffer4010010100508037BPstINEBuffer3+BSA757510050508037CPstl-HFNEBuffer4107550100258037CPvuINEBuffer3+BSA107510010100No37BPvuI-HFNEBuffer4+BSA25100100100100No37BPvuIINEBuffer2100100100100100No37BPvuII-HFNEBuffer4025010008037BEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentRsalNEBuf

34、fer410010050100156537ARsrIINEBuffer4257510100256537CEnzymetopSuppliedNEBufferB1B2B3B4EcoRIHeatInac.Incu.Temp.DiluentSacINEBuffer1+BSA1005010100156537ASacI-HFNEBuffer4+BSA1005010100156537ASacIINEBuffer42575101001006537ASallNEBuffer3+BSA001000506537ASalI-HFRE-Mix-NRNRNRNR-8037-SalI-HFNEBuffer410100100

35、1001006537ASapINEBuffer475500100506537BSau3AINEBuffer1+BSA100501010056537ASau96INEBuffer450100100100508037ASbfINEBuffer475500100256537ASbfI-HFNEBuffer45025010006537BScaINEBuffer3NRNR100ddNR508037AScaI-HFRE-Mix-NRNRNRNR-6537-ScaI-HFNEBuffer410010010100156537BScrFINEBuffer41001001001001006537CSexAINEB

36、uffer4+BSA1007550100506537ASfaNINEBuffer307510025506537BSfcINEBuffer4+BSA755010100256537BSfiINEBuffer4+BSA01001010015No50CSfoINEBuffer42510050100100No37BSgrAINEBuffer41005010100506537ASmaINEBuffer400010006525ASmlINEBuffer4+BSA25752510050No55ASnaBINEBuffer4+BSA25502510058037ASpelNEBuffer4+BSA7510025100508037CSpelRE-Mix-NRNRNRNR-8037-Spel-HFNEBuffer4+BSA2

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