Trichostatin-A-TSA-DataSheet-MedChemExpress_第1頁
Trichostatin-A-TSA-DataSheet-MedChemExpress_第2頁
Trichostatin-A-TSA-DataSheet-MedChemExpress_第3頁
Trichostatin-A-TSA-DataSheet-MedChemExpress_第4頁
全文預(yù)覽已結(jié)束

下載本文檔

版權(quán)說明:本文檔由用戶提供并上傳,收益歸屬內(nèi)容提供方,若內(nèi)容存在侵權(quán),請進(jìn)行舉報或認(rèn)領(lǐng)

文檔簡介

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemETrichostatin ACat. No.: HY-15144CAS No.: 58880-19-6Synonyms: TSA分式: CHNO分量: 302.37作靶點(diǎn): HDAC作通路: Cell Cycle/DNA Damage; Epigenetics儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 25 mg/mL (8

2、2.68 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 3.3072 mL 16.5360 mL 33.0721 mL5 mM 0.6614 mL 3.3072 mL 6.6144 mL10 mM 0.3307 mL 1.6536 mL 3.3072 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度,選擇合適的溶劑配制儲備液,并請注意儲備液的保存式和期限。體內(nèi)實(shí)驗(yàn) 請根據(jù)您的實(shí)驗(yàn)動物和給藥式選擇適當(dāng)?shù)娜芙獍?,配制前請先配制澄清的儲備液,再依次添加助溶?為保證實(shí)驗(yàn)結(jié)果的可靠性,體內(nèi)實(shí)驗(yàn)的作液,建議您現(xiàn)現(xiàn)配,當(dāng)天使

3、;澄清的儲備液可以根據(jù)儲存條件,適當(dāng)保存;以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占):1. 請依序添加每種溶劑: 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (8.27 mM); Suspended solution; Need ultrasonic2. 請依序添加每種溶劑: 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (8.27 mM); Clear solution1/3 Master of Small Molecules 您邊

4、的抑制劑師www.MedChemEBIOLOGICAL ACTIVITY物活性 Trichostatin A (TSA)有效的,特異的組蛋去?;割愋?I 和 II (HDAC class I/II) 抑制劑,對 HDAC 的IC50 值為 1.8 nM。IC50 & Target HDAC1.8 nM (IC50)體外研究 Trichostatin A is a potent and specific inhibitor of HDAC class I/II, with an IC50 value of 1.8 nM for HDAC.Trichostatin A (TSA) inhibit

5、s proliferation of eight breast carcinoma cell lines with meanSD IC50 of124.4120.4 nM (range, 26.4-308.1 nM). HDAC inhibitory activity of Trichostatin A is similar in all cell lineswith mean IC50 of 2.40.5 nM (range, 1.5-2.9 nM) 1. Trichostatin A (330 nM) increases Gs proteinexpression in human myom

6、etrial cells, but does not increase Gs mRNA levels 2. Trichostatin A (20-75nM)induces minimal cytotoxicity to adipose-derived stem cells (ADSCs), and enhances the osteogenicdifferentiation capacity of ADSCs 3. In addition, Trichostatin A (0, 10, 100, 500 nM) dose-dependentlydecreases HDAC class I/II

7、 activity 4.體內(nèi)研究 Trichostatin A (500 g/kg, s.c.) pronounces antitumor activity without causing any measurable toxicity indoses of up to 5 mg/kg by s.c. injection, in randomized controlled efficacy studies using the N-methyl-N-nitrosourea carcinogen-induced rat mammary carcinoma model 1.PROTOCOLCell

8、Assay 3 Cells are cultured in a 96-well plate at 1103 cells per well with 100 L complete DMEM in the presence orabsence of a HDAC inhibitor Trichostatin A for 72 h. Cytotoxicity is measured by performing WST-8 assayusing a CCK-8 cell proliferation kit. The 450 nm absorbance is measured with a microp

9、late reader. Allexperiments are carried out in triplicate and 3 independent experiments are performed 3.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Rats 1Administration 1 Twelve rats are randomized to receive 500 g/kg Trichostatin A in 50 L D

10、MSO, or 50 L DMSO as vehiclecontrol, by s.c. injection twice weekly for 4 weeks. In subsequent studies, 30 rats are randomized to receiveTrichostatin A 500 g/kg in 50 L DMSO, or 50 L DMSO as vehicle control, by s.c. injection daily for 4weeks. Weekly tumor measurements, estimated tumor volumes, and

11、body mass are recorded for eachanimal. Animals are sacrificed at the end of the 4-week study period; palpable tumors are resected andimmediately snap-frozen in liquid nitrogen. Animals with tumors 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)

12、表的科研獻(xiàn) Proc Natl Acad Sci U S A. 2016 Sep 6;113(36):9967-76. Kidney Int. 2017 Sep;92(3):669-679. Theranostics. 2019 Apr 13;9(9):2424-2438.2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE Cell Death Dis. 2018 Apr 27;9(5):497. J Mol Med (Berl). 2019 Jun 14.See more customer validations on HYPERLINK /

13、www.MedChemEREFERENCES1. Vigushin DM et al. Trichostatin A is a histone deacetylase inhibitor with potent antitumor activity against breast cancer in vivo. ClinCancer Res. 2001 Apr;7(4):971-6.2. Karolczak-Bayatti M, et al. Expression of the GTP-Binding Protein Gs in Human Myometrial Cells is Regulat

14、ed by Ubiquitination andProtein Degradation: Involvement of Proteasomal Inhibition by Trichostatin A.,Reprod Sci. 2012 Aug 8.3. Hu X, et al. Histone deacetylase inhibitor trichostatin A promotes the osteogenic differentiation of rat adipose-derived stem cells byaltering the epigenetic modifications on Runx2 promoter in a BMP signaling-dependent manner.,Stem Cells Dev. 2012 Aug 8.4. Azechi T, et al. Trichostatin A, an HDAC class I/II inhibitor, promotes Pi-induced vascular calcification via up-regulation of the expressionof alkaline phosphatase. J Atheroscler Thr

溫馨提示

  • 1. 本站所有資源如無特殊說明,都需要本地電腦安裝OFFICE2007和PDF閱讀器。圖紙軟件為CAD,CAXA,PROE,UG,SolidWorks等.壓縮文件請下載最新的WinRAR軟件解壓。
  • 2. 本站的文檔不包含任何第三方提供的附件圖紙等,如果需要附件,請聯(lián)系上傳者。文件的所有權(quán)益歸上傳用戶所有。
  • 3. 本站RAR壓縮包中若帶圖紙,網(wǎng)頁內(nèi)容里面會有圖紙預(yù)覽,若沒有圖紙預(yù)覽就沒有圖紙。
  • 4. 未經(jīng)權(quán)益所有人同意不得將文件中的內(nèi)容挪作商業(yè)或盈利用途。
  • 5. 人人文庫網(wǎng)僅提供信息存儲空間,僅對用戶上傳內(nèi)容的表現(xiàn)方式做保護(hù)處理,對用戶上傳分享的文檔內(nèi)容本身不做任何修改或編輯,并不能對任何下載內(nèi)容負(fù)責(zé)。
  • 6. 下載文件中如有侵權(quán)或不適當(dāng)內(nèi)容,請與我們聯(lián)系,我們立即糾正。
  • 7. 本站不保證下載資源的準(zhǔn)確性、安全性和完整性, 同時也不承擔(dān)用戶因使用這些下載資源對自己和他人造成任何形式的傷害或損失。

評論

0/150

提交評論