從基因組學(xué)到蛋白質(zhì)組學(xué)

Genomicsisbig,Proteomicsisenormous從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第1頁(yè)!TheHumanGenomeisencodedby23chromosomepairsTheseencode~20,300differentgenesc.2001從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第2頁(yè)!DNAisposedof4chemicalbases(nucleicacids)A,G,C,TDNAdoublehelixThe“simple”chemistryofDNAhasindustrialisedgenomics從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第3頁(yè)!1stgenome:$70MNow:$50Kanddecreasingrapidly從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第4頁(yè)!CaterpillarButterflyGenomevs.ProteomeGenomesarestatic,ProteomesaredynamicHeartLiver從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第5頁(yè)!Proteinsareposedofupto20aminoacids-DifferentphysicalandchemicalpropertiesProteinscanfoldtoformuniquestructurestoprovidecellularspecificityLargedynamicrangeofcellularproteinlevels106從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第6頁(yè)!TheHumanProteome20,000genesAssumeeachproteincontains4sitesofpost-translationalmodification2^4x20,000=320,000proteinspeciesTheproteomeisenormouslylargeandplex從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第7頁(yè)!ProteomicsatMacquarieUniversityBirthplaceoftheterm‘proteomics’–Wilkins1994HomeofAustralianProteomeAnalysisFacility(APAF)since1995LargestarrayofproteomicinstrumentationinAustraliaforresearchandservices5leadingresearchgroups(Baker,Hancock,Haynes,Molloy,Packer)50MacquarieUniversitystaff/studentsusingAPAFannuallyforresearch從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第8頁(yè)!2DGelofurinaryproteinsNativeEPORebinantEPOismorealkalineDetectionofdrugsforsportsanti-dopingKhanetal.2005從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第9頁(yè)!CanweidentifybiomarkersofearlyresponsetochemotherapyinmetastaticCRC?ColorectalcancerchemotherapyCurrentclinicalparadigmistoassesspatientsbyCTscan6weeksafterpletionoftherapyR.Murthyetal.2006從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第10頁(yè)!VascularProteinTargetingofAVMsM.StoodleyHighriskofdeathuponruptureManyAVMsareuntreatablebysurgery從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第11頁(yè)!從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第12頁(yè)!HemoglobinAntibodyEpidermalGrowthFactorReceptorProteinsaremolecularmachinesofmanyshapesandsizesRubisco從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第13頁(yè)!20,300proteincodinggenes30%lackexperimentalevidencefortheirexistence230cellsinhumans.EachcellhasadifferentproteomeVerylittleinformationabout:abundancecelltypedistributionsubcellularlocalisationInteractionswithotherproteinsFunction!TheHumanProteome從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第14頁(yè)!ProteomicsisthestudyoftheproteinsinagenomeatagiventimeDeterminingtheidentityoftheproteinsDeterminingtheamountoftheproteinsDeterminingtheinteractionbetweenproteins從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第15頁(yè)!ExampleApplicationsofProteomicsIdentifyproteinsinbloodfordiseasediagnosisorprognosisIdentifyproteinsinplantsthathelpwithsalttoleranceordroughttoleranceBio-prospectingtheenvironmenttoidentifyproteinswithdesirablepropertiesTobetterunderstandtheroleofproteinsinhumandiseaseprocessesInanti-sportsdopingtocatchdrugcheats從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第16頁(yè)!ColorectalcancerchemotherapyCanweapriorideterminewhichpatientswillbenefitfromchemotherapy?AcquireproteomicdataIdentifycandidatebiomarkersValidatebiomarkersAllStageCpatientsundergochemo,yetonly50%needitOfthosethatneedit,whowillbenefit?從基因組學(xué)到蛋白質(zhì)組學(xué)共19頁(yè)，您現(xiàn)在瀏覽的是第17頁(yè)!TargetedMassSpectrometryAssaytoQuantitatePlasmaProteinsEachcolouredpeakrepresentsadifferentproteinmeasuredfrom<1uLplasma從基因組學(xué)到蛋白質(zhì)