iFSP1-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEiFSP1Cat.No.:HY-136057CASNo.:150651-39-1分?式:C??H??N?分?量:323.35作?靶點(diǎn):Ferroptosis作?通路:Apoptosis儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:20mg/mL(61.85mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM3.0926mL15.4631mL30.9262mL5mM0.6185mL3.0926mL6.1852mL10mM0.3093mL1.5463mL3.0926mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2mg/mL(6.19mM);Suspendedsolution;Needultrasonic1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性iFSP1FSP1(AIFM2)的選擇性有效抑制劑，EC50值為103nM。iFSP1可選擇性地誘導(dǎo)過(guò)表達(dá)FSP1的GPX4敲除細(xì)胞發(fā)?鐵死亡(ferroptosis)。iFSP1可增強(qiáng)多種?癌細(xì)胞系對(duì)鐵死亡誘導(dǎo)劑的敏感度，如:(1S,3R)-RSL3(HY-100218A)。IC50&TargetEC50:103nM(FSP1)[1]體外研究iFSP1(0.001-1μM;24hours)inhibitstheGpx4-knockoutcellgrowthasadose-dependentmanner,butdoesnotinhibitthewildtypecellgrowth.TreatmentwiththeferroptosisinhibitorLip-1protectsGPX4-knockoutcellsfromiFSP1-inducedferroptosis[1].iFSP1(0.001-1μM;24hours)islessefficientthangeneticdeletionofFSP1,whereasiFSP1treatmentintheFSP1-knockoutbackgroundhadnoadditiveeffecttoRSL3-inducedferroptosis[1].iFSP1(3μM;24hours)treatmentresultsinanobvioustoxicityofRSL3inapanelofgeneticallyengineered(FSP1-knockout)humancancercelllines[1].AIFM2:theflavoproteinapoptosis-inducingfactormitochondria-associated2isapreviouslyunrecognizedanti-ferroptoticgene.AIFM2,whichisrenamedferroptosissuppressorprotein1(FSP1)[1]CellProliferationAssay[1]CellLine:Wild-typeandGpx4-knockoutPfa1orHT1080cellsoverexpressingFSP1–HAConcentration:0.001-1μMIncubationTime:24hoursResult:Wastoxictocellsthatdependsolely(noGPX4expressiondetectable)onFSP1function.CellViabilityAssay[1]CellLine:NCl-H1437,NCl-H1437FSP1KO,U-373,U-373FSP1KO,MDA-MB-436,MDA-MB-436FSP1KO,SW620,SW620FSP1KO,MDA-MB-435S,MDA-MB-435SFSP1KO,A549andA549FSP1KOConcentration:3μMIncubationTime:24hoursResult:Sensitizedavarietyofhumancancercelllinestotheferroptosisinducer(1S,3R)-RSL3.戶(hù)使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?CellDiscov.2022May3;8(1):40.?Small.2021Aug;17(32):e2101368.?CellDeathDis.2022May18;13(5):468.?CellDeathDis.2022Jan21;13(1):73.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCES[1].DollS,etal.FSP1isaglutathione-independentferroptosissuppressor.Nature.2019Nov;575(7784):693-698.McePdfHeightCaution:Producthasnotbeenfullyvalidatedfor