ABD957-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEABD957Cat.No.:HY-142161分?式:C??H??F?N?O?S分?量:627.68作?靶點(diǎn):MAGL作?通路:MetabolicEnzyme/Protease儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:25mg/mL(39.83mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制備儲備液1mM1.5932mL7.9658mL15.9317mL5mM0.3186mL1.5932mL3.1863mL10mM0.1593mL0.7966mL1.5932mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?，-20°C儲存時(shí)，請?jiān)?個(gè)?內(nèi)使?。BIOLOGICALACTIVITY?物活性ABD957?種有效和選擇性的去棕櫚酰酶ABHD17家族的共價(jià)抑制劑，抑制IC50的IC50為0.21μM。ABD957可以阻斷N-Ras信號傳導(dǎo)和NRAS突變AML細(xì)胞的?長。IC50&TargetIC50:0.21μM(ABHD17B)[1]體外研究ABD957(500nM;pretreatedfor1h)attenuatesN-RasdepalmitoylationinAMLcells[1].1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEABD957(0.11nM-10μM;72h)blocksthesignalingandproliferationofNRAS-mutantleukemiacells[1].CellProliferationAssay[1]CellLine:OCI-AML3,THP1,HL60,NB-4andNOMO1cellsConcentration:0.1,1,10,100,1000,10000nMIncubationTime:72hoursResult:ReducedthegrowthofNRAS-mutantAMLcelllines(OCI-AML3,THP1andHL60),butnotKRASmutantcelllines(NB-4andNOMO1).REFERENCES[1].RemsbergJR,et,al.ABHD17regulationofplasmamembranepalmitoylationandN-Ras-dependentcancergrowth.NatChemBiol.2021Aug;17(8):856-864.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792/p>