LED光源在園藝與藥用植物和人工蟲(chóng)草室內(nèi)培養(yǎng)中的應(yīng)用課件

LED光源在園藝與藥用植物和

人工蟲(chóng)草室內(nèi)培養(yǎng)中的應(yīng)用,2014年6月21日，廣州

2014年植物生長(zhǎng)調(diào)節(jié)劑與現(xiàn)代農(nóng)業(yè)研討會(huì)植物的種子萌發(fā)、幼苗生長(zhǎng)、形態(tài)建成、開(kāi)花結(jié)果等過(guò)程都需要光不同的生物及不同生長(zhǎng)發(fā)育期需要不同的光質(zhì)與光強(qiáng)研究?jī)?nèi)容非洲菊組織培養(yǎng)不定芽誘導(dǎo)、芽增殖和生根鐵皮石斛和金釵石斛組培繁殖原球莖增殖、生長(zhǎng)、分化蟲(chóng)草子實(shí)體形成,生長(zhǎng),功能成分子實(shí)體的誘導(dǎo)形成生長(zhǎng)：高、鮮重、干重生物活性物質(zhì):蟲(chóng)草素,多糖,類胡蘿卜素I-Gerbera

hybrida組織培養(yǎng)Xidian

Liu

劉西典Jianzong

Peng

彭建宗Xiaojing

Wang

王小菁Results

—

shoot

multiplicationCompared

to

WFL,

no

significant

differences

were

observed

in

the

treatments

of

V1,V2,

V5

and

V6.

V3

and

V4

decreased

the

FW

of

the

shoots.

V6

increaseddry

weight

significantly.Fresh

weight

and

dry

weightHeight

and

number

of

shootsAll

LEDs

could

increase

the

height

of

shoots

except

V5.

But

No.ofshoots/explant

had

no

significant

different.ChlorophyllcontentAll

LEDs

induced

chlorophyll

biosynthesis

significantly,compared

with

WFLW

V1V4

V3V6

V2

V5WFL35

days

cultured

in

vitro

under

LEDExplant:DedifferentiationcallusRedifferentiationRootingPlantletsResults

—

Root

inductionReceptacle

Multiplication

DivisionAdventitious

budsCluster

shoots

Root

inductionunderLED

Single

shootV3

treatment

decreased

FW

and

DW.V2

treatment

increased

FW

and

DW

significantly.Fresh

weight

and

Dry

weightV3,

V4

and

V5

promoted

the

root

activity

significantly.V2

increased

the

maximum

root

length.Root

activity

and

maximum

root

lengthV1V2V3V4V5V6WFLSeedlings

35

d

after

rooting

inductionSurvivalrateHeight

(cm)No.

of

leavesV1V2V3V4V5V6WFL100%100%100%100%100%100%100%9.46±0.22

a9.77±0.22

a8.19±0.34

b9.54±0.30

a8.02±0.27

b8.25±0.48

b7.09±0.23

c5.19±0.23

b4.94±0.11

b6.06±0.19

a6.00±0.35

a5.31±0.24

b6.19±0.20

a4.60±0.27

bResults

—

Transplantation15

days

after

transplantation,no

difference

on

seedlings

survival

rates.AllLEDtreatedseedlings

were

significant

higher

andnumberof

leaves

increased.?

Among

6

LEDs,

V1,V2

and

V6

were

suitable

for

shootmultiplication

and

V2

was

good

for

root

induction.?

Survival

rates

of

seedlings

from

all

LED

treatmentshad

no

differenceafterseedling

transplantation.

Butthe

seedlings

grown

under

LEDs

were

higher

withmore

leaves

than

WFL.ConclusionsJie

Ye

葉婕Hongmei

Bao

包紅梅QingshengYe

葉慶生II-Dendrobium

officinale組培繁殖Tissue

culture

processshootsPlantlets(1-2cm)seedling

(7-8cm)germination

1

monthgrowth

and

differentiation

2

month

seeds

growth1

month

protocormsshoot

elongation

3

monthPlantlets27LED

treatmentDW

of

plantlet(g)DW

of

plantlets

under

V6

was

significantly

higher

than

others

after

90days

treatment.30

days60

days90

days0.000.010.020.03ab

ababbaaaaa

aaV1V2V3V4V6V5FLabacabcaba

aabDW

of

plantlets

cV6Height

of

plantlet(cm)

There

were

some

different

in

plantlet

height

after

30

and

60

days

irradiationunder

LED,

the

different

disappeared

after

90

days.30days60days90days0.50.01.52.0V1V2V4V6V3V5FLaaaababaac

bcaaaaa

bcbcabc

bcHeight

of

plantlets

a90天后差異不顯著Roots

of

plantletAfter

60

days

cultivation,

there

were

more

roots

under

V6.60days90days204V1V3V2V4V5V6FLa

aaaacdaab

bdabcaRootsnumberperplantlets

aGrowth

of

plants

after

30days

under

LED.Growth

of

plants

after

60

dayscultivation

under

LED.Growth

of

plants

after

90

dayscultivation

under

LED.?

Among

6

LED,

V6

were

suitable

for

plantlet

growth,it

promoted

the

DW,

diameter,

tillering

androoting

in

Dendrobium

officinale.ConclusionsIII-Cordyceps

militarisZhiliYi

易智莉Haihang

Li

李海航本實(shí)驗(yàn)室培養(yǎng)的蛹蟲(chóng)草選出對(duì)蛹蟲(chóng)草的生長(zhǎng)、產(chǎn)品質(zhì)量最優(yōu)的光質(zhì)，為L(zhǎng)ED光源在蛹蟲(chóng)草栽培中的運(yùn)用提供理論和應(yīng)用基礎(chǔ)。不同的光照時(shí)間蛹蟲(chóng)草色素的變化初步探究蟲(chóng)草色素的光誘導(dǎo)形成過(guò)程與機(jī)理。重復(fù)3次原基誘導(dǎo)形成材料與方法材料與方法重復(fù)3次子實(shí)體生長(zhǎng)實(shí)驗(yàn)材料與方法甲醇浸提光照組合光質(zhì)紅/藍(lán)/遠(yuǎn)紅1遠(yuǎn)紅光/2紅光/3紅光+藍(lán)光+遠(yuǎn)紅光紅∶藍(lán)∶遠(yuǎn)紅=4∶2∶44紅光+藍(lán)光+遠(yuǎn)紅光紅∶藍(lán)∶遠(yuǎn)紅=3∶2∶55紅光+藍(lán)光+遠(yuǎn)紅光紅∶藍(lán)∶遠(yuǎn)紅=3∶3∶46紅光+藍(lán)光+遠(yuǎn)紅光紅∶藍(lán)∶遠(yuǎn)紅=1∶4∶57紅光+藍(lán)光+遠(yuǎn)紅光紅∶藍(lán)∶遠(yuǎn)紅=1∶6∶38藍(lán)光/9白光紅∶藍(lán)∶遠(yuǎn)紅=6∶3∶110熒光燈（對(duì)照）紅∶藍(lán)∶遠(yuǎn)紅=4∶6∶0LED光照處理結(jié)果與討論RFrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/31.

黑暗、紅光、遠(yuǎn)紅光下不能形成子實(shí)體原基2.

藍(lán)光和含藍(lán)光的LED組合都能誘導(dǎo)原基形成。表明藍(lán)光在子實(shí)體原基誘導(dǎo)形成中起決定性作用3.

LED白光（R/B=2/1）和LED處理Ⅲ（R/B=2/1）對(duì)原基的誘導(dǎo)形成較好1.光質(zhì)對(duì)子實(shí)體原基形成的影響RFrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/3處理Ⅲ鮮重最高其他LED處理組與熒光對(duì)照組無(wú)顯著差異遠(yuǎn)紅光下鮮重最低結(jié)果與討論2.光質(zhì)對(duì)子實(shí)體生長(zhǎng)（鮮重）的影響RFrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/3Ⅲ（R/B/Fr=4/2/4）干重較高遠(yuǎn)紅光下干重最低,其次是紅光Ⅲ-Ⅹ處理都含藍(lán)光，干重?zé)o顯著差異，但都高于遠(yuǎn)紅光和紅光結(jié)果與討論3.光質(zhì)對(duì)子實(shí)體生長(zhǎng)（干重）的影響結(jié)果與討論各處理的子實(shí)體平均高度無(wú)統(tǒng)計(jì)學(xué)差異處理Ⅲ的高度略高于其它RFrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/34.光質(zhì)對(duì)子實(shí)體生長(zhǎng)（平均高度）的影響I.FR(100%)II.R(100%)VII.R:B:FR1:6:3VIII.B(100%)III.R:B:FR4:2:4IV.R:B:FR3:2:5V.R:B:FR3:3:4 VI.R:B:FR1:4:5IX.LEDwhiteX.FluorescentLED白光蟲(chóng)草素含量最高，比對(duì)照高62%；其次是LED紅光，比對(duì)照高60%其他LED組合比熒光對(duì)照都有不同程度的提高。紅光有利于提高蟲(chóng)草素的含量，藍(lán)光對(duì)蟲(chóng)草素積累的作用不顯著。RFrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/3結(jié)果與討論5.光質(zhì)對(duì)蟲(chóng)草素含量的影響62%60%各光照處理下蟲(chóng)草多糖的含量無(wú)統(tǒng)計(jì)學(xué)差異。RFrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/3結(jié)果與討論6.光質(zhì)對(duì)蟲(chóng)草多糖含量的影響RFrBWFLR/B/Fr=4/2/4R/B/Fr=3/2/5R/B/Fr=3/3/4R/B/Fr=1/4/5R/B/Fr=1/6/3LED藍(lán)光下類胡蘿卜素含量最高，比對(duì)照組提高了51.6%。LED遠(yuǎn)紅光下類胡蘿卜素含量最低，比熒光對(duì)照低54.3%。其次是紅光，比對(duì)照低46.4%。藍(lán)光和含藍(lán)光的LED處理，類胡蘿卜素都比對(duì)照高，且藍(lán)光比例越高，類胡蘿卜素含量越高。結(jié)果與討論7.光質(zhì)對(duì)類胡蘿卜素含量的影響R光誘導(dǎo)色素合成光誘導(dǎo)子實(shí)體原基形成（形態(tài)建成）色素的形成發(fā)生在光