為大腦半球結構中主要的灰質部分之一

IntrastriataltargetsofprojectionfibersfromthecentrallateralnucleusoftheratthalamusNoritakalchinohe,Hiroyasulwatsuki,KazuhikoShoumuraNeuroscienceLetters302(2001)105-108IntroductionThebasalgangliaandthecerebellumarethemajorsubcorticalstructuresthatareimportantforregulationandcontrolofvoluntarymovementProjectionfromthedeepcerebellarnucleitothemotor-relatedStriatum,whichreceivesinputsfromthemotorcorticalareasHavebeenreportedtoberelayedatseveralthalamicnuclei;theventrallateralnucleusparscaudails,ventralposteriorlateralnucleusparsoralis,areaXandcentrallateralnucleus(CL)inthemonkey,andtheventrallateralnucleusandCLintheratBasalganglia為大腦半球結構中主要的灰質部分之一，也就是neuron之所在分為：Caudatenucleus（尾狀核）&Putamen(被殼）StriatumCaudatenucleus&putamen合稱striatum（紋狀體）因經過的axon把他們弄出了條紋的外觀臨床上，basalganglia是指striatum,視丘下部神經核,黑質與運動功能異常有關，特徵，不自主，無目的的運動IntroductionPreciseunderstandingofthefunctionofthesecerebello-thalamo-striatalprojectionsrequiresdetailedknowledgeofintrastriataltargetsofprojectionfibersfromthecerebello-recipientthalamicnucleiInthepreviousstudyWedemostratedthatthalamostriatalneuronsintheCLreceivedirectstrongprojectionsfromthelateralcerebellarnucleusintheratSpecialattentiontoa.whetherornotprojectionfibersfromtheCLmakesynapsesondendriticspines,whichareagoodmakerfor

striatalprojectionneuronsb.whetherornotprojectionfibersfromtheCLareinsynapticcontactwithparvalbumin(PV)immunopostivestriatalinterneuron,whichrepresentthemajorclassof

GABAergicinterneuronsinthestriatumMethoda.TenmaleWistarratswereanesthetizedwithsodiumPentobarbital(70mg/kg,I.P)b.10%solutionofbiotindextranamine(BDA)in0.01MPB(pH7.2)wasiontophoreticallyinjectedintotheCLc.Theglassmicropipette(tipdiameter:15μm)usinga

positivecurrent(5-7μA,7son/7soff)for20~30minsSurvivalTheperiodof7days,theratswerere-anesthetizedandperfusedtranscardiallywith100mlofsalinefollowedby500mlofparaformaldehydeand0.1%glutaraldehydein0.1MPB(pH7.4)Post-fixedinthesamefixativeandstoredovernightat4℃inPB,thefollowingday,thebrainwasseriallycut50μmthickonamicroslicerStaining(fordextran)1.wash2.ABCkit(1:100~1:200dilute)for2hatroomtemperature3.wash4.0.1%DABin0.05MTrisand0.003%H2O2BDA-labeledaxonswerevisualizedasbrownStaining(forPV)PVimmunohistochemistryusingVIPasachromogen1.wash2.mouseanti-PVmonoclonalantibodydiluted1:1000for24hat4℃3.1:200biotinylatedhorseanti-mouseIgGantibody2h4.1:100ABCkitfor2h5.VIPkitPVimmunopositivestructureswerevisualizedaspurpleResultInjectionsiteToevaluteTherelativefrequencyoftheCLboutonstoallexcitatoryinputsonPVimmunopositiveinterneurons,werecordedbothBDA-labeledandunlabeledboutonswhichmadeasymmerticalsynapticspecializationswithPVimmunopositivestructuresResultBDA-labeledfibersweredistributedmainlyinthelaterodorsalpartofthestriatumandintermingledwithPVimmunopositiveneuronsAllBDA-labeledfibersweresmallindiameter(0.1~0.3μm)andexhibitedanextendedtypeofarborizationwithlong,straight,relativelyunbranchedtrajectoriesthroughneuropilResultOf408BDA-labeledterminals,371(90.9%)wereinsynapticcontractwithPVimmunonegativedendriticspines,35(8.6%)madesynapsesonPVimmunonegativedendriticshafts,andonlytwo(0.5%)formedsynapsesonPVimmunopositive

dendriticshaftsDiscussionInthepresentstudy,wedemostratedthatalargeproportion(91%)ofthalamostriatalfibersfromtheCLterminatedonPVimmunonegativedendriticspines,whichwereassumedtobethoseofstriatalprojectionneuronsThusThetargetpreferenceofthalamostriatalfibersfromtheCLseemsnottohaveasignificantbiastointerneuronsoverprojectionneuronsinthestriatumInsummaryThecerebello-CL-striatalprojectionhavepredominantaccess(probablyexcitatory)tostriatalprojectionneuro