基于柔性基底酶型生物燃料電池物研究中期報告

基于柔性基底酶型生物燃料電池物研究中期報告（BasedonMid-termReportontheStudyofEnzymaticBiofuelCellwithFlexibleSubstrate）摘要：柔性基底是一種新型的生物燃料電池（BFC）載體，能夠提高BFC的靈活性和性能。本研究旨在探討不同柔性基底對BFC性能的影響，并研究BFC中酶的類型和濃度對性能的影響。實驗結(jié)果表明，不同柔性基底對BFC的性能影響顯著，其中PDMS基底的BFC性能最優(yōu)。此外，在相同的基底下，較高的酶濃度可以提高BFC輸出電流。因此，優(yōu)化酶的類型和濃度對于提高BFC性能具有重要意義。關(guān)鍵詞：柔性基底；生物燃料電池；酶濃度；輸出電流Abstract:Flexiblesubstrateisanovelcarrierforbiofuelcell(BFC),whichcanimprovetheflexibilityandperformanceofBFC.ThisstudyaimstoinvestigatetheinfluenceofdifferentflexiblesubstratesonBFCperformance,andtostudytheeffectofenzymetypeandconcentrationonperformance.TheexperimentalresultsshowthatthedifferentflexiblesubstrateshavesignificanteffectsonBFCperformance,amongwhichthePDMSsubstratehasthebestBFCperformance.Inaddition,underthesamesubstrate,higherenzymeconcentrationcanimproveBFCoutputcurrent.Therefore,optimizingthetypeandconcentrationofenzymesisofgreatsignificancetoimproveBFCperformance.Keywords:Flexiblesubstrate;Biofuelcell;Enzymeconcentration;Outputcurrent.Introduction:Biofuelcells(BFCs)areapromisingalternativeenergysourceduetotheiruniqueadvantages,suchashighenergyconversionefficiency,lowpollutionandrenewableenergy.BFCscandirectlyconvertbiochemicalenergyintoelectricalenergythroughelectrochemicalreactions,andthesubstratesusedinBFCshaveasignificantimpactontheirperformance.FlexiblesubstratesareanewtypeofsubstratethatcanimprovetheflexibilityandperformanceofBFCs.Flexiblesubstrateshavemanyadvantages,includinglowweight,highflexibilityandgoodbiocompatibility,whichmakethemsuitableforuseinwearabledevices.However,theinfluenceofdifferentflexiblesubstratesonBFCsperformanceandtheeffectofenzymetypeandconcentrationonperformanceneedtobefurtherstudied.Objectives:TheobjectivesofthisstudyaretoinvestigatetheinfluenceofdifferentflexiblesubstratesonBFCperformance,andtostudytheeffectofenzymetypeandconcentrationonperformance.Methods:BFCswithdifferentflexiblesubstrates(PDMS,siliconrubber,andTPU)werefabricatedandtheirperformanceswereevaluatedbycyclicvoltammetry(CV)andchronoamperometry.TheBFCswiththebestperformancewereselectedforfurtherstudy.Next,theeffectofenzymetypeandconcentrationonBFCperformancewasstudied.Differenttypesofenzymes(glucoseoxidase,laccase,andhorseradishperoxidase)wereusedinBFCswithPDMSsubstrate,andthecorrespondingBFCperformanceswereevaluated.Results:TheresultsshowedthatthedifferentflexiblesubstrateshavesignificanteffectsonBFCperformance.Amongthem,thePDMSsubstratehasthebestBFCperformance.ThepeakcurrentdensityofPDMS-basedBFCwas48.50μA/cm2,whichwas2.4timesthatofsiliconrubber-basedBFC(20.26μA/cm2)and4.3timesthatofTPU-basedBFC(11.18μA/cm2).TheeffectofenzymetypeandconcentrationonBFCperformancewasalsostudied.TheresultsshowedthathigherenzymeconcentrationcanimproveBFCoutputcurrent.Forexample,whenglucoseoxidase(GOx)concentrationwasincreasedfrom0.05mg/mLto0.1mg/mL,theoutputcurrentofBFCwasincreasedby1.3times,from5.34μA/cm2to6.94μA/cm2.Conclusion:Inconclusion,theresultsofthisstudyshowedthatdifferentflexiblesubstrateshavesignificanteffectsonBFCperformance,andPDMSsubstratehasthebestBFCperformance.In