多功能影像軌跡行為系統(tǒng)

會(huì)議流程產(chǎn)品介紹應(yīng)用原理產(chǎn)品搭配與運(yùn)用產(chǎn)品介紹FeaturePhotobeamVideoSpatialresolution(openfield50x50cm)16x16beams3.2cmbetweenbeams500x500pixels0,1cmbetweenpixelsRawdataBeamcrossingsDigitalimage#animals/arenaSingleSingleormultipleMultiplearenas/sensorNoYesMultipleBodyPointsNoYes(nose,center,tailbase)EthoVisionXT自動(dòng)化的動(dòng)物軌跡追蹤及行為分析軟體所有迷宮都適用（Watermaze,Plusmaze,radialarmmaze,Openfield…）自動(dòng)化排程＝更進(jìn)一步的實(shí)驗(yàn)設(shè)定精準(zhǔn)的物件判別技術(shù)（鼻、身體、尾部，輪廓）論文發(fā)表數(shù)量最多產(chǎn)品介紹即時(shí)分析：經(jīng)由影像擷取卡擷取攝影影像事後分析：

利用以拍攝好的影片檔(MPEG,AVI)產(chǎn)品介紹數(shù)位化影像解析度:擷取卡決定解析度的大小灰階應(yīng)用原理如何抓取影像特徵?物件偵查利用像素原理物件判別移除多餘雜訊干擾抓取物件面積也可辨識(shí)多個(gè)物件物件特徵分析X,Y位置(persample)物件面積(#ofpixels)活動(dòng)力Mobility(%change)應(yīng)用原理應(yīng)用原理基本架構(gòu)OpenfieldWatermazeElevatedplusmazeObjectrecognitionRadialarmmazeHoleboardSocialinteractiontestForcedswimtestTailsuspensiontestOperantconditioningActivityin96-wellplate利用最新的影像追蹤技術(shù)，您將得到最可信賴的數(shù)據(jù)多種物件辨別方式：

GrayscalingStaticsubtractionDynamicsubtractionDifferencingColortracking應(yīng)用原理GrayscalingSubtraction底圖－即時(shí)影像＝物件應(yīng)用原理軌跡

(e.g.distancemoved,velocity)位置

(inzone,distancetozone/point,zonetransition,headingtopoint)軌跡形狀

(turnangle,heading)個(gè)體行為

(mobility,movement,rotation,elongation)社交行為

(relativedistance,relativemovement)Trialcontrol&Hardwarerelatedparameters

(e.g.#oflicks,pelletsdropped,interval‘light-on=>behavior’)能夠在其他電腦上重複分析數(shù)據(jù)，並將其數(shù)據(jù)輸出成Excel檔（*.xls）或是Text檔（*.txt）供其他統(tǒng)計(jì)軟體使用參數(shù)選擇應(yīng)用原理模組應(yīng)用產(chǎn)品搭配與運(yùn)用MultipleBodyPointsModule

允許追蹤物件面積的重心、鼻子、尾部，三個(gè)部位活動(dòng)性及延展性的分析產(chǎn)品搭配與運(yùn)用MultipleArenaModule

同時(shí)追蹤100個(gè)區(qū)域

可用於同時(shí)觀察多個(gè)迷宮各個(gè)區(qū)域可有不同排程進(jìn)行實(shí)驗(yàn)

可用於斑馬魚(yú)、果蠅、昆蟲(chóng)等需要多量數(shù)據(jù)的實(shí)驗(yàn)

產(chǎn)品搭配與運(yùn)用PhysiologicalsignalsModule

能輸入外部的生理訊號(hào)，使動(dòng)物的生理訊號(hào)與其行為軌跡同步分析產(chǎn)品搭配與運(yùn)用利用獨(dú)一無(wú)二的演算法，抓取動(dòng)物身上的小標(biāo)記，進(jìn)而分析動(dòng)物軌跡（鼻子、身體、尾部），避免動(dòng)物交疊後誤判的產(chǎn)生最多同時(shí)偵測(cè)16隻動(dòng)物於同一區(qū)域分析:動(dòng)物之間的親近度靠近或離開(kāi)（閃避）彼此可將軌跡與參數(shù)結(jié)合，同步顯示於影片SocialinteractionModule產(chǎn)品搭配與運(yùn)用簡(jiǎn)單易懂的圖解邏輯介面方式來(lái)定義實(shí)驗(yàn)流程可利用參數(shù)來(lái)定義實(shí)驗(yàn)start/stop狀態(tài)可利用TTL觸發(fā)方式，控制外部裝置多元化的觸發(fā)控制，取代只有on/off的觸發(fā)NoldusUSBIO-Box:最多支援同時(shí)24個(gè)TTL訊號(hào)輸出TrialandHardwareControl

Module產(chǎn)品搭配與運(yùn)用WaterMazeUseEthoVisiontoautomatewatermazeexperiments.Calculatetotaldistancemoved,timetillreachingplatformetc.Automaticallystoprecordingwhenanimalhasreachedplatform產(chǎn)品搭配與運(yùn)用OpenFieldUsezonestodistinguishbetweenedgeandcenterCalculatetimespentineachzoneandtotaldistancemovedComparebetweentwodifferenttreatments/mousestrains產(chǎn)品搭配與運(yùn)用PlusmazeMakeazoneforeacharmandoneforthecentreCalculatetimespentinopenarmsversustimespentinclosedarmsCountnumberof‘headdips’產(chǎn)品搭配與運(yùn)用Traditionalway:ManualObservations,largeinter-observerbias.Difficulttoreproduceresults.UsingEVMobilityparameter:

-StandarderrorofdatascoredbyEthoVisionissmallerthanscoredintraditionalway.-AutomaticallydetectwhenanimalbecomesfatiguedPorsoltSwimTest產(chǎn)品搭配與運(yùn)用PlacepreferencetestStudyplacepreferencebehaviorofananimal;MakeazoneofeachseparateareaandcalculatetimespentineachzoneStudyLight-darkpreferenceGivedrugandconfineanimaltocertainarea(e.g.withverticalstripes)thenreleaseanimalandseeifheprefersoravoidsthisarea產(chǎn)品搭配與運(yùn)用OtherapplicationsInsecttrackingPlacepreferenceForagingbehaviorPredator-preyinteraction(e.g.searchingefficiencypredator)Avoidanceof/attractiontopheromones產(chǎn)品搭配與運(yùn)用OtherapplicationsHumanSpatialOrientationandWay-findingAnalysiswithEthoVisioninaRealArenaMaze產(chǎn)品搭配與運(yùn)用總結(jié)WhyuseEthoVisionXT?利用標(biāo)準(zhǔn)化的方式，減少人為誤差利用