DB34T 4223-2022 黃緣閉殼龜　規(guī)程

34Yellow-marginedBoxTurI本文件按照GB/T1.1—2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分：標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)定增、方國(guó)俠、沈保平、高凌、徐麗、彭步旭、鮑鳴、張四剛1黃緣閉殼龜本文件給出了黃緣閉殼龜（CuoraflavomarginataGray，1863）的名稱與分類、主要形態(tài)構(gòu)造特征、生長(zhǎng)和繁殖特性、遺傳學(xué)特征、檢測(cè)方法GB/T18654.2養(yǎng)殖魚類種質(zhì)檢GB/T18654.12養(yǎng)殖魚類種質(zhì)檢驗(yàn)GB/T18654.15養(yǎng)殖魚類種質(zhì)檢驗(yàn)第4名稱與分類5.1.1外形體呈橢圓形，前部略窄、后部略寬。頭光滑無(wú)鱗，鼓膜圓而清晰，頭部背面呈橄欖色，兩眼后各2椎盾：5枚。肋盾：8枚，左右各4枚。緣盾：22枚，左右各11枚。臀盾：2枚，左右各1枚。35.1.2.3g，背甲長(zhǎng)104.6mm～121.2mm，年齡4+～6+。各注：各可量性狀說(shuō)明參長(zhǎng)，胃分化明顯，十二指腸上附有條形白色胰臟，無(wú)盲腸。雌雄異體，雄性睪丸1對(duì)，淡白色，卵圓形，位于腎臟前端內(nèi)側(cè)，后端為輸精管，通泄殖腔，陰莖位于尾基部的泄殖孔內(nèi)；雌體卵巢1對(duì)，位46.1生長(zhǎng)背甲寬6.2繁殖自然條件下，雌龜為6性成熟，雄龜是5性成熟。每年5月～8月產(chǎn)卵，5月中旬～6月上旬為產(chǎn)卵高峰期。一年多次產(chǎn)卵，一般每年產(chǎn)卵1次～4次。每只雌龜每年產(chǎn)卵1枚/次～4枚/次。7.1.2染色體核型見(jiàn)圖3,染色體組型見(jiàn)圖4。5黃緣閉殼龜線粒體16SrRNA基因片段的堿基序列為1596bp,種內(nèi)K2P遺傳距離為0.0024,小于2%,標(biāo)準(zhǔn)序列見(jiàn)附錄C。69判定規(guī)則7Wt1=12.73e0.476（r=0.9843）·················································Lt=45.47T0.466（r=0.9944）········································Wt2=34.81T0.461（r=0.9939）·····················································(A.3)Wt2——t齢時(shí)龜背甲寬，單位為毫米（mmWt1=Lt3.018（r=0.9899）··························································(Wt1——t齢時(shí)龜體重，單位為克（gLt=0.82Wt21.191（r=0.9925）·············································Wt2——t齢時(shí)龜背甲寬，單位為毫米（mm8DB34/T4223—6jooobM9黃緣閉殼龜線粒體16SrRNA基因標(biāo)準(zhǔn)序列（1596b1GCAAGAACCTAGCCCAACCAAAATAAAAAACTTAACAAACAAAACTATCC51CACCAAAACCAACCAAAACATTCTCACCATCCTAGTATAGGCGATAGAAA101AGACAGTTGGAGCAATAGAGATAGTACCGCAAGGGAAAGATGAAAAACAA151TGAAATATTCACTAAGCCATAAAAAGCAAAGATTAATCCTTATACCTTTT201GCATCATGATTTAGCTAGCATATTCAAGCAAAGAGCCCTAAAGTATGAAA251CCCCGAAACTAAGTGAGCTACTTAAAGGCAGCCACACCAAGGCTAAATCC301GTCTCTGTGGCAAAAGAGTGGAAAGACCTGTAAGTAGAGGTGAAAAGCCT351AACGAACTTAGTGATAGCTGGTTGCTCAATAAAAGAATATAAGTTCAACC401TTAAACCTTCTAAAAACAACCTAAAGTATAAAAGAAAAGTTTAAGATTTA451TTCAATTGGGGTACAGCCCAATTGAAAAAGGATACAACCAATAACGGAGG501ACAAAACCCCAAAAATAACAACCGTAGGCCTTAAAGCAGCCACCAACTAA551GAAAGCGTCAAAGCCCACCCGCTATTAAATAGTAACAAAAAATTTTTTCC601CCTAATAATATTGAGCTACTCCATTTAAATAGAGGAATTAATGCTAAAAT651GAGTAACAAGAAGACAAAACTTCTCTAATACGCTAGCTTAAGTCATAACA701GATAAACTACTGACTATTAACATCCATCACTATAAAACAACAACATTTTA751AATCACCCTATAGACCTAACTGTTAACCCAACACAGGAGCGTATACAAGA801AAGATTAAAATTTGTAAAAGGAACTAGGCAAATAAACGAGCCCGACTGTT851TACCAAAAACATAGCCCCTAGCAACACAAGTATTAGGGGTAATGCCTGCC901CAGTGACACTGTTAAACGGCCGCGGTATCCTAACCGTGCAAAGGTAGCGT951AATCACTTGTCTTTTAAATAAAGACTAGAATGAATGGCCAAACGAGGTTC1001TACCTGTCTCTTACAAATAATCAGTGAAATTGATCTTCCTGTGCAAAAGC1051AGGAATAACATTATAAGACGAGAAGACCCTGTGGAACTTCAAATACAAAT1101CAACTATTATCAACACCCACCTACGGGTCCACATCAAACTAGCATCTGAC1151TTATATTTTCGGTTGGGGCGACCTCGGAGTAAAATAAAACCTCCGAAAAA1201GAATTTCCTTTTAAACCTAGACCTACAGCCCACAGTGCTTCCAGCAAAAC1251GATCCAATATACTTGATCAACGAACCAAGCTACCCCAGGGATAACAGCGC1301AATCCCATCCTAGAGTTCCTATCGACGATGGAGTTTACGACCTCGATGTT1351GGATCAGGACATCCTGATGGTGCAACTGCTATCAAGGGTTCGTTTGTTCA1401ACGATTAACAGTCCTACGTGATCTGAGTTCAGACCGGAGCAATCCAGGTC1451GGTTTCT