食源性肽粉通則編制說(shuō)明

《食源肽粉通則》編制說(shuō)明制修訂計(jì)劃，項(xiàng)目編號(hào)20221342-T-424，項(xiàng)目名2024年7月-11月，開(kāi)展分子量分布的試驗(yàn)方法驗(yàn)證工作，建立了食源性肽粉的分處于2021年向國(guó)標(biāo)委提交了標(biāo)準(zhǔn)修訂計(jì)“本文件規(guī)定了食源性肽粉的術(shù)語(yǔ)和定義，規(guī)定了食源性肽粉的原輔材料、感官、理化容，同時(shí)給出了產(chǎn)品分類(lèi)。本文件適用于食源性肽粉產(chǎn)品的生產(chǎn)、檢驗(yàn)和銷(xiāo)售。”1GB/T22729-20082QB/T4707-20143QB/T5298-20184GB31611-20235SB/T10634-20116QB/T4588-20137GB31645-20188GB/T22492-2008910按照GB/T1.1-2020規(guī)范標(biāo)準(zhǔn)文本描述。分別對(duì)色澤、滋味氣味和狀態(tài)進(jìn)行了描述表1感官要求表2本標(biāo)準(zhǔn)的理化指標(biāo)要求蛋白質(zhì)（以干基計(jì)g/100g≥肽含量（以干基計(jì)g/100g≥表3食源性肽粉相關(guān)標(biāo)準(zhǔn)的蛋白質(zhì)含量要求GB/T22492-2008X≥90X≥85X≥80QB/T5298-2018/X≥90X≥75/QB/T4707-2014X≥80GB/T22729-2008≥14.5(總氮)≥13.5(總氮)≥13.5(總氮)GB31645-201815(總氮)SB/T10634-2011≥90≥85≥85QB/T4588-2013≥15(總氮)≥13(總氮)≥85≥10(總氮)GB31611-2023≥11.2(總氮)表4行業(yè)征集食源性肽粉相關(guān)樣品的蛋白質(zhì)情況（以干g/100g（以干g/100gSCFF-PP-01SCFF-PP-19SCFF-PP-20SCFF-PP-03/SCFF-PP-21SCFF-PP-2291.50SCFF-PP-05SCFF-PP-239.32SCFF-PP-2491.1011.60SCFF-PP-2593.70SCFF-PP-2683.30SCFF-PP-27SCFF-PP-1011.80SCFF-PP-30SCFF-PP-12SCFF-PP-31SCFF-PP-13SCFF-PP-32SCFF-PP-14SCFF-PP-33SCFF-PP-1579.20SCFF-PP-3477.30SCFF-PP-1691.70SCFF-PP-3592.50SCFF-PP-1797.50SCFF-PP-3623.60SCFF-PP-1897.20SCFF-PP-3778.307.2.2肽含量表5食源性肽粉相關(guān)標(biāo)準(zhǔn)的肽含量要求GB/T22492-2008X≥80X≥70X≥55QB/T5298-2018/X≥75X≥50/QB/T4707-201470GB/T22729-2008X≥85X≥75X≥80GB31645-2018/SB/T10634-2011857555QB/T4588-20137075GB31611-2023表6行業(yè)征集食源性肽粉相關(guān)樣品的肽含量情況SCFF-PP-0173.18SCFF-PP-1984.14SCFF-PP-02SCFF-PP-2051.36SCFF-PP-0394.31SCFF-PP-2167.2894.77SCFF-PP-22SCFF-PP-0512.41SCFF-PP-2310.22SCFF-PP-2476.36SCFF-PP-0713.93SCFF-PP-2576.31SCFF-PP-2692.6775.49SCFF-PP-2781.46SCFF-PP-1012.29SCFF-PP-30SCFF-PP-12SCFF-PP-31SCFF-PP-1358.75SCFF-PP-3291.21SCFF-PP-1463.15SCFF-PP-33SCFF-PP-15SCFF-PP-3467.83SCFF-PP-1682.79SCFF-PP-35SCFF-PP-1794.11SCFF-PP-3622.15SCFF-PP-18SCFF-PP-3761.60GB20371-2016《食品加工用植物蛋白粉》為≤10.0（12.0）以外，蛋白肽產(chǎn)品的標(biāo)準(zhǔn)水檢測(cè)，結(jié)果表明，樣品的水分在2.3~9.7g/100g之間（見(jiàn)表7），其中表7食源性肽粉相關(guān)標(biāo)準(zhǔn)的水分要求GB20371-2016≤10.0(12.0)GB/T22492-2008≤7.0QB/T5298-2018≤6.5GB/T22729-2008≤7.0GB31645-2018≤7.0QB/T4707-2014≤7.0QB/T4588-2013≤7.0SB/T10634-2011≤7.0≤7.0GB31611-2023≤7.0表8行業(yè)征集食源性肽粉相關(guān)樣品的水分情況SCFF-PP-016.77SCFF-PP-205.18SCFF-PP-022.34SCFF-PP-213.90SCFF-PP-223.23SCFF-PP-056.61SCFF-PP-237.44SCFF-PP-242.44SCFF-PP-07SCFF-PP-254.01SCFF-PP-266.12SCFF-PP-27SCFF-PP-10SCFF-PP-282.81SCFF-PP-118.55SCFF-PP-295.45SCFF-PP-12SCFF-PP-30SCFF-PP-136.51SCFF-PP-312.61SCFF-PP-14SCFF-PP-32SCFF-PP-15SCFF-PP-33SCFF-PP-16SCFF-PP-34SCFF-PP-175.22SCFF-PP-35SCFF-PP-185.05SCFF-PP-367.53SCFF-PP-193.10SCFF-PP-377.2.4重均分子量表9行業(yè)征集食源性肽粉相關(guān)樣品的重均分子量情況SCFF-PP-01873.45SCFF-PP-191,268.17726.68SCFF-PP-20405.73SCFF-PP-032,208.95SCFF-PP-211,135.162,920.12SCFF-PP-221,101.03SCFF-PP-05653.98653.98SCFF-PP-23453.15453.15592.92592.92SCFF-PP-24542.83552.49552.49SCFF-PP-25388.54388.543,854.22SCFF-PP-26561.00561.00988.35988.35SCFF-PP-273,695.96SCFF-PP-10SCFF-PP-30839.50SCFF-PP-12661.62SCFF-PP-31734.72SCFF-PP-13542.27SCFF-PP-321,354.78SCFF-PP-14777.25SCFF-PP-331,127.65SCFF-PP-15931.43931.43SCFF-PP-34647.59SCFF-PP-164,168.97SCFF-PP-351,012.09SCFF-PP-171,514.57SCFF-PP-36772.74SCFF-PP-18SCFF-PP-371.2.1.1酸溶蛋白1.2.1.2游離氨基酸表1-1肽粉中的肽含量141.84g/100g1.2.2.1酸溶蛋白的檢出限CL=s×t（n-1，1-α=0.99）×V=s×3.143×V（1）s——試驗(yàn)標(biāo)準(zhǔn)偏差；3.143——取n=7且置信水平1-α=0.99時(shí)查t分布表得：t（n-1，1-α=0.99）=3.143；V——試樣體積，mL。表1-2檢出限檢測(cè)結(jié)果。0.1860.1118500.5190.111810.4310.340.148500.5130.111810.22500.5190.111810.42500.1118500.5150.111810.30500.5190.111810.42500.5210.1118），LOD=（2）S——組分峰高；N——基線(xiàn)噪音；C——組分摩爾質(zhì)量，nmol。表1-3方法的檢出限和定量限Asp133.1105020.143119.1105014448220.145105.1105014325620.230.1470.77147.1105011177120.410.1881.3875.1105017184320.140.122Ala89.1105012355620.0510.230.170Val117.1105020.530.3011.76240.2105014914710.021149.2105017529920.120131.2105011338820.1851.21131.2105013529420.310.1551.02181.2105013402920.1571.42165.2105010948520.1921.58155.2130021029420.124Lys106.212084820.530.3311.76Arg174.28507814520.570.2181.89115.19584621.440.835表1-4氨基酸標(biāo)準(zhǔn)曲線(xiàn)及相關(guān)系數(shù)AspY=5.375e+004+3.441e+006XY=5.071e+004+3.517e+006XY=6.502e+004+3.43e+006XY=-1.498e+005+3.606e+006XY=3.33e+005+4.324e+006XAlaY=-753.3+3.174e+006XValY=6.195e+005+2.334e+006XY=1.951e+005+3.727e+006XY=-3.38e+004+3.349e+006XY=-1.557e+005+2.497e+006XY=-2.926e+005+3.249e+006XY=1.714e+004+3.84e+006XY=-7.765e+004+4.155e+006XY=-6393+3.722e+006XLysY=9.382e+004+3.299e+006XArgY=-1.008e+005+3.293e+006XY=8.446e+005+2.445e+006X1.2.1.1酸溶蛋白表1-5準(zhǔn)確度檢測(cè)結(jié)果0.1830.10582.0501.6170.105842.4842.110.35712.0501.5930.105841.772.0501.6030.105841.071.2.4.2游離氨基酸表1-6方法回收率Asp67396771.9495.2567776721.9195.6365594941.891.9668259141.9394.8385912301.911.90100.52Ala6362997Val47094551.751.9092.2375010051.961.94101.0465421061.961.90103.3449794501.98103.8762722621.98102.0569855601.811.9294.5275880271.841.9495.1070173981.8990.73Lys65485631.96Arg1.9652100701.791.9890.181.2.5.1酸溶蛋白表1-7樣品中酸溶蛋白重復(fù)性檢測(cè)結(jié)果0.11180.186樣品-10.11182.41041.70841.891.410.05770.11182.62141.313樣品-30.11182.50042.2630.05250.11182.42941.824樣品-50.11182.65642.8720.11182.54841.3651.2.5.2游離氨基酸表1-8肽粉中游離氨基酸重復(fù)性檢測(cè)結(jié)果（單位：g/100g）123456RSD（%）Asp0.2300.2530.2310.2370.2300.2353.950.1590.1710.1520.1650.1570.1580.1604.170.1940.1820.1990.1960.1930.1953.910.1810.1850.1810.1960.1700.1900.1844.83Val0.1330.1420.1350.1290.1430.1310.1364.274.780.2210.2370.2150.2300.2330.2273.560.2310.0124.720.3120.0134.410.3270.3580.3120.3510.3420.3330.3370.0174.97Lys0.3100.3300.3160.0154.953.950.2150.2310.2120.2150.2140.21