17-博士研究生課——生命科學前沿進展——胰腺的發(fā)育—-魏熾炬.ppt

1、胰腺的發(fā)育,魏熾炬,The Development of Pancreas,Helena Edlund,E8,E10,The Development of Pancreas,Helena Edlund,The Structure of Pancreas,Helena Edlund,Transcriptional Specification,Micheal German,Transcriptional Specification,Micheal German,Early Hormone Expression,Jacob Hecksher-Sorensen,Adult Insulin- and G

2、lucagon-Producing Cells Differentiate From Two Independent Cell Lineages,Pedro Luis Herrera Development 127, 2000,Paper One,Islet Precursor Cell Labeling,Translational STOP,Brian Sauer,The Beta Cell Lineage,The Alpha Cell Lineage,Both Alpha and Beta Cells Derive From PDX-1 Expressing Precursors,Dire

3、ct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors,Guoqiang Gu, Jolanta Dubauskaite and Douglas A. Melton Development 129, 2002,Paper Two,Generation of The Transgenic Mice,PDX1+ Progenitors Give Rise to Islets, Acini and Duct in Adult Mic

4、e,Wild Type HPAP-,pdx1-cre HPAP+,pdx1-cre-ER,pGK-cre HPAP+,pdx1-cre Laz/Vimentin,E9.5/TM 8 week,NGN3+ Precursor Cells Give Rise to Islets,ngn3-cre,NGN3+ cells contribute to the renewal of islets in adult mice,ngn3-cre-ER,8 week TM,Day 1,Day 1,Day 7,Day 0,Notch Signaling Controls Multiple Steps of Pa

5、ncreatic Differentiation,L. Charles Murtaugh, Ben Z. Stanger, Kristen M. Kwan, and Douglas A. Melton PNAS December 9, 2003,Paper Three,Notch Signaling and Pancreas Development,ngn3,X,Cre-activated misexpression of activated Notch1,Cre-activated misexpression of activated Notch1,E9.5,Cre Activation B

6、y PDX1 Promoter,E13.5/TM E17.5,E11.5/TM E15.5,Cre Activation at E11.5-E13.5,Cre Activation By Ngn3 Promoter,E15.5,E13.5,Late Cre Activation,Adult/TM,Control,Adult pancreatic -cells are formed byself-duplication rather than stem-celldifferentiation,Yuval Dor, Juliana Brown, Olga I. Martinez 而陰性等位基因在活性染色體細胞有突變表型.,X染色體的失活涉及Xist RNA的穩(wěn)定,它能將失活染色體包被起來.,X-gal staining for -gal activityin the intestinal crypts,Homozygous Female,Hetrozygous Female,Neonatal islets were heterog