中科院2006年攻讀碩士學(xué)位研究生入學(xué)試題《生物化學(xué)及分子生物學(xué)》.doc

2006203012341-45G6789A5SRNA5SRNA5SRNA1011Q12131415CD4HIV1617mRNASD23SRNA318-6-1920DNA30451A BC D2L-A B C50% D3DNA AKlenow BTaq CDNA DDNA4Fe A B C D5 A B C D6 A- B- C- D7A-CoA+3NAD+FAD+H2OCoASH+3NADH+2H+FADH2+2CO2B-CoA+3NADH+FAD+H2OCoASH+3NAD+2H+FADH2+2CO2C-CoA+3NAD+FAD+Pi+GDP+2H2OCoASH+3NADH+2H+FADH2+GTP+2CO2D-CoA+3NADH+FAD+Pi+GDP+2H2OCoASH+3NAD+2H+FADH2+GTP+2CO28 ANorthern blot BWestern blot CSouthern blot DEastern blot9A B C D10e-tRNAe CAMPe +ATPe CAMP+PPie CAMP+tRNAe CtRNA+AMP32PPPi32PATPaa-tRNAAATP32PPi BtRNAATP32PPi Ce ATP32PPi De AMP32PPi11A B C D12 A B C D13SDSA BC D14A BC D15ATPATPA2Na+2K+B2K+2Na+C3Na+2K+D3K+2Na+16AATP BATP CCa2+ATP DADP-ATP17 A B C D18DixonA B C D19AA B CQ D20ABCA BATPC D21A26-16-B26-CATP D22A B C DD-23 A BC D24DNA ABCD25DNA17m15m A6.67103 B5.88103 C5.55103 D5.2610326 DNAADNARNABDNACRNADDNA27ABmRNACmRNADmRNA28DNARNAA-32PdATP B-32PATP C3HTdR D32Pi29CDP- ABCD3020cm4cm4cm A3 B4 C5 D65251Leu-Pro-Ser-Trp-Ala200.01M Tris-HClPH7.023415NDNADNA15NDNA5ADNABDNADNADNA55012AKmVmax0.1mM100U/mg1U1mBKmKcat5m50min-150kDa3ALDL4Thomas Cech RNArRNAThomas Cech 51. MESADFYEVE PRPPMSSHLQ SPPHAPSNAR LWLWSPGRGPR AAPSPTCRPGAAGRICEHET61 SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAAGPAGG GGDFDYPGAPAGPGGAVMSA 121 GAHGPPPGYG CAAAGYLDGR LEPLYERVGA PALRPLVIKQEPREEDEAKQ LALAGLFPYQ 181 PPPPPPPPHP HASPAHLAAP HLQFQIAHCGPTPPPTPVPS PHAAPALGAA GLPGPGSALK 240 GLAGAHPDLR TGGGGGGGSGAGAGKAKKSVD PDKAKQRNVE LTQQKVELTS DNDRLRKRVE 301 QLSREADTLRGIFRQLPESS VKLAMATARL ARGGGTLAGR PPGLWRPRGW FRVAGSGCPG 361RASQD40kDa80kDa20061+ 2+ 3- Kcat4+5- RasGRas6+ 7+ 8-9+10- 11-12+ 13+14-15+ CD4HIVHIVHIVHIVHIV16+17- 16sRNA18- -6-19+20+1B 2A L-3C4D5D6D7C8B9D10D11D12C13C14C15C16D17C 18D19B20B ABC2ATPABCATPATP21B22C23A24C25C 17-15100010.4/3.54=588026C27A28C29A30B20-4/4=41Trpmax=280280nmTrp348nm21PP11PP1-26-3CC18184DNAN15N144DNAN153N145DNAATCG20DNAdNTP DNADNA1 T50602Km v=Kcat/KmESKcatA=100U/mg=100umol/mgmin=100umol50kDa/0.001mol=5000