生物信息學(xué)教學(xué)資料：引物設(shè)計(jì)

1、引物設(shè)計(jì)生科1201班周宇箏3702120110現(xiàn)在要對h9n2病毒的基因設(shè)計(jì)引物。一.利用ncbi查找該病毒的全部基因1 進(jìn)入ncbi的genebank界面。display settings: © tabular 20 per page. sort by relevancesond to:2輸入病毒的名字h9n2,查找相關(guān)的基因。gene sources genomiccategoriesresults: 1 to 20 of 22«first < prg page 1 ot2 next> lastalternately spliced_annotated g

2、ns protein-codingname/gene id descriptionlocationaliases hahemagglutinin influenza a virusnc.004908.1 (32 1714)fluavahhh9n2s4gp1sequence contentid： 1460996(a/hong kong/10799(h9n2)ccds ensembl refseq ns1 10:1460995nonstructural protein 1 influenza a virus (a/hong kong/i073/99(h9n2)nc.004906 1 (27 719

3、)fluavahhh9n2s8gp2refseqgene m2 id: 1461002matnx protein 2 influenza a vjojs (among kong/10739(h9n2)nc.004907.1 (33.1014)fluavahhh9n2s7gp1status current onlyu eb2id: 1460998polymerase pb2 influenza a virus (miong kong/10739(h9n2)nc_004910.1 (28.2307)fluavahhh9n2s1gp1chromosome locations moren oaid：

4、1460997neuraminidase influenza a wes (a/hong kong/10739(h9n2)nc.004909 1 (1 1404)fluavahhh9n2s6gp1clear all np id: 4036076nucleoprotein unflwnza a virus (a/hong kong/10739(h9n2)nc_004905 2 (40 1536)fluavahhh9n2s5gp1show additional filters nep id: 4036374nonstructural protein 2 influenza a vjojs (a/h

5、ong kong/i073/99(h9n2)nc.004906 1 (27. 864)fluavahhh9n2s8gp1ns2 ml id 1461001matrix protein 1 influenza a virus (a/hong kong/10739(h9n2)nc.004907.1 (33 791)fluavahhh9m2s7gp2n兇広id： 13229457pa-x protein influenza a virus (a/hong kong/10739(h9n2)nc.004912 1 (21 780)fluavahhh9n2s3gp23以該病毒的一個基因h9n2ha為例，進(jìn)

6、行引物設(shè)計(jì)atggagacagtatcactaataactatactactagtagcaacagtaagcaatgcagataaaatctgcatcggctatcaatcaacaaactccacagaaactgtggacacactaacagaaaacaatgtccctgtgacacatgccaaagaactgctccacacagagcataatgggatgctgtgtgcaacaagcttgggacaacctcttattttggacacctgc acaattgaagggctaatctatggcaatccttcttgtgatctatcgctggaaggaagagaatggtcctatatcgtc

7、gagag accatcagctgttcacggattgtgttaccccgggaacgtagaaaatctagaagagctaaggtcactttttagttctgcta ggtcttatcaaagaatccagattttcccagacacaatctggaatgtgtcttacgatgggacaagcacagcatgctcaggttcattctacagaagcatgagatggttgactcgaaagaacggcgattaccctatccaagacgcccaatacacaaataatcaagggaagaacattcttttcatgtggggcataaatcacccacccaccgatacta

8、cacagagagatctgtacacgagaaccgacacaacaacgagtgtggcaacagaagaaataaataggatcttcaaaccattgataggaccaaggcctcttgtcaacgg tttgatgggaagaattgattattattggtctgtattgaaaccgggtcaaacactgcgaataaaatctgatgggaatctaatagctccatggtatggacacattctttcaggagagagccacggaagaattctgaagactgatttaaaaaggggtagctg cacagtgcaatgtcagacagagaaaggtggctt

9、aaacacaacactgccattccaaaatgtaagtaggtatgcatttggaaa ctgctcaaaatacattggcataaagagtctcaaacttgcagttggtctgaggaatgtgccttctagatctagtagaggactattcggggccatagcagggtttatagagggaggttggtcaggactagttgctggttggtatgggttccagcattcaaatg accaaggggttggtatggcagcagatagagactcaacccaaaaggcaattgataaaataacatccaaagtgaataatata gtcgacaa

10、aatgaacaagcagtatgaaatcattgatcatgaattcagtgaggtagaaactagacttaacatgatcaataat aagattgatgatcaaatccaggatatatgggcatataatgcagaattgctagttctgcttgaaaaccagaaaacactcg atgagcatgacgcgaatgtaaacaatctatataataaagtaaagagggcgttgggttccaatgcggtggaagatgggaa aggatgtttcgagctataccacaaatgtgatgacca/ytgcatggagacaattcggaacgggacct

11、acaacagaaggaag tatcaagaggagtcaaaattagaaagacagaaaatagagggggtcaagctggaatctgaaggaacttacaaaatcctcac catttattcgactgttgcctcatctcttgtgattgcaatggggtttgctgccttcttgttctgggccatgtccaatgggtcttgcag/ytgcaacatttgtatataa用primers軟件為這些基因設(shè)計(jì)引物。1.首先安裝一個primers軟件2點(diǎn)擊file下拉菜單，選擇new,然后選擇dna sequencefile edit view function translate window helpnewopeninsert sequencesavesave asprefere ncesdegenerate basesprintquitctrl+sctrurqrlfb3.將已經(jīng)查到的基因序列復(fù)制到文本框中。4.因?yàn)樽罱K得到的基因其目的是插入到質(zhì)粒中，因此設(shè)計(jì)引物前需要 進(jìn)行酶切位點(diǎn)分析，所以要點(diǎn)擊enzyme按鈕，選擇add添加口標(biāo)酶, 然后點(diǎn)擊0k進(jìn)彳亍分析。5