9基因工程抗體和抗體工程

1、C genes code for the constant regions of immunoglobulin protein chains.V gene is sequence coding for the major part of the variable (N-terminal) region of an immunoglobulin chain.Immunoglobulin genes are assembled from their parts in lymphocytes Figure 24.4 Heavy and light chains combine to generate

2、 an immunoglobulin with several discrete domains.Immunoglobulin genes are assembled from their parts in lymphocytesFigure 24.4 Heavy and light chains combine to generate an immunoglobulin with several discrete domains.Immunoglobulin genes are assembled from their parts in lymphocytesTable 24.1 Each

3、immunoglobulin family consists of a cluster of V genes linked to its C gene(s).Immunoglobulin genes are assembled from their parts in lymphocytes FamilyV GenesC GenesManMouseManMouseLambda64Kappa100098Figure 24.5 The lambda C gene segment is preceded by a J segment, so that V-J recombination generat

4、es a functional lambda light-chain gene.Immunoglobulin genes are assembled from their parts in lymphocytesFigure 24.6 The kappa C gene segment is preceded by multiple J segments in the germ line. V-J joining may recognize any one of the J segments, which is then spliced to the C gene segment during

5、RNA processing.Immunoglobulin genes are assembled from their parts in lymphocytesFigure 24.7 Heavy genes are assembled by sequential joining reactions. First a D segment is joined to a J segment; then a V gene segment is joined to the D segment.Immunoglobulin genes are assembled from their parts in

6、lymphocytesFigure 24.8 The lambda family consists of V gene segments linked to a small number of J-C gene segments.The diversity of germline information Figure 24.9 The human and mouse kappa families consist of V gene segments linked to 5 J segments connected to a single C gene segment.The diversity

7、 of germline information Figure 24.10 A single gene cluster in man contains all the information for heavy-chain gene assembly.The diversity of germline information Figure 24.12 Consensus sequences are present in inverted orientation at each pair of recombining sites. One member of each pair has a sp

8、acing of 12 bp between its components; the other has 23 bp spacing.Recombination between V and C gene segments generates deletions and rearrangements Figure 24.13 Breakage and reunion at consensus sequences generates immunoglobulin genes.Recombination between V and C gene segments generates deletions and rearrangementsFigure 15.8 Reciprocal recombination between direct repeats excises the material between them; each product of recombination has one copy of the direct repeat.Recombination between V and C gene segments generates deletions and rearrangeme