各種激發(fā)光和吸收光波長(zhǎng)

1、現(xiàn)在發(fā)現(xiàn)一個(gè)更全的有關(guān)激發(fā)光和發(fā)射光的波長(zhǎng)圖譜。這對(duì)CONFOCAL去做多重染色時(shí)是必不可少的參考資料。全部文件Excitati on and Emissi on Wavele ngths of FluorophoresTavi's "FluoroTable": Com mon fluorophores(from )FluorophoreAbsorpti onEmissi on Other info1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluore

2、sce in5045295-Carboxyfluorescein (5-FAM)4925185-Carbox yn apthofluoresce in (pH 10)512/598563/668 Ratio Dye, pH5-Carboxytetramethylrhodam ine542568(5-TAMRA)5-FAM (5-Carboxyfluoresce in)4925185-HAT (Hydroxy Tryptami ne)370-415520-5405-Hydroxy Tryptami ne (HAT)370-415520-5405-ROX (carboxy-X-rhodami ne

3、)5786045675915-TAMRA548552(5-Carboxytetramethylrhodam ine)5425686-Carboxyrhodam ine 6G5185436-CR 6G5185436-JOE5205487-Ami no-4-methylcoumari n3514307-Ami noacti no mycin D (7-AAD)5466477-Hydroxy-4-methylcoumari n360449, 4559-Ami no-6-chloro-2-methoxyacridi ne412,430471,474ABQ344445Acid Fuchs in54063

4、0ACMA412,430471,474(9-Ami no-6-chloro-2-methoxyacridi ne)Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Oran ge, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Y ellow470550Acriflavin436520Acriflavin Feulge n SITSA355-425460Aequorin (Photoprote in)466PhotoproteinA

5、FPs - AutoFluorescentProtein-(Qua ntum Biotech no logies) see sgGFP,sgBFPAlexa Fluor 350346442342441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa Fluor 546556, 557572, 573Alexa Fluor 568577,578603Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650Alexa Flu

6、or 647647666Alexa Fluor 660668698Alexa Fluor 680679702Alizarin Complex on530-560,580,580624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Ami no methylcoumar in)345425347444AMCA-X353442Amino act ino myc in D555655Amino coumari n346442350445Amino methylcoumarin

7、(AMCA)345425347444Ani lin Blue600An throcyl stearate360-381446APC (Allophycocya nin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530 Ratio Dye, Zn2+APTS424505Astrazon Brillia nt Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Y ellow 7 GLL450480Atabrine4364

8、90ATTO-TAG CBQCA465560ATTO-TAG FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisami nophe nyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberi ne Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)381,382, 445,blue shifted GFPBlue Flu

9、oresce nt Prote in383447, 448(Y 66H)Blue Fluoresce ntProteinBFP/GFP FRETBima ne398490Bisbe nzamide360461Bisbe nzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505 Ratio Dye, Zn2+Blan cophor FFG390470Bla ncophor SV370435BOBO-1462481BOBO-3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy

10、500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodipy Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE52

11、8547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO-1462481BO-PRO-3544570Brillia nt Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529 Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532 Ratio Dye, Zn 2+Calcei n49451

12、7Calcei n Blue373440Calcium Crims on588, 589611,615Calcium Gree n501, 506531Calcium Gree n-1 Ca2+ Dye506531Ca2+ DyeCalcium Gree n-2 Ca2+506/503536Ca2+Calcium Gree n-5N Ca2+506532Ca2+Calcium Gree n-C18 Ca2+509530Ca2+Calcium Orange549575576Calcofluor White385,395, 437,405440, 445Carboxy-X-rhodami ne (

13、5-ROX)576601Cascade Blue377420398423399Cascade Y ellow399550400552Catecholam ine410470CCF2 (Ge neBlazer)CFDA494520CFP - Cyan Fluoresce nt Protein430,433, 474,Cyan Fluoresce nt436, (453)475,Protein476,(501)CFP/YFP FRETChlorophyll480650Chromomyc in A436-460470Chromomyc in A445575CL-NERF (Ratio Dye, pH

14、)504/514540Ratio Dye, pHCMFDA494520Coele nteraz ineCa2+Dye, (429)465Ca2+Dye,biolumin esce neebiolu min esce nee,n ative moleculeCoele nteraz ine ep (Ca2+ Dye,)(430)442Ca2+Dye,biolu min esce neeCoele nteraz ine f(437)473Ca2+Dye,biolu min esce neeCoele nteraz ine fcp452Ca2+Dye,biolu min esce neeCoele

15、nteraz ine h(437)464Ca2+Dye,biolu min esce neeCoele nteraz ine hcp(433)444Ca2+Dye,biolu min esce neeCoele nteraz ine ip441Ca2+Dye,biolu min esce neeCoele nteraz ine n(431)467Ca2+Dye,biolu min esce neeCoele nteraz ine O460575Coumar in Phalloidin387470C-phycocya nineCPM Methylcoumari n384469Methylcoum

16、ar inCTC400-450602CTC Formaza nCy24895068554568581598Cy35145665525705548649666675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453) 475(501)cyclic AMP Fluorose nsor (FiCRhR)500517CyQua nt Cell Proliferati on Assay480520CellProliferati onAssayDabcyl453Dan syl340578Dan syl Ami ne337517Dan syl Cadaver

17、i ne335518Dan syl Chloride372518Dan syl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH(Dichlorodihydrofluoresce in505535Diacetate)DDAO463607DHR (Dihydorhodami ne 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488605498713DiA (4-Di-16-ASP)45659

18、1Dichlorodihydrofluoresce inDiacetate505535(DCFH)DiD - Lipophilic Tracer644665LipophilicTracerDiD (DiIC18 (5)644665DIDS341415Dihydorhodami ne 123 (DHR)505535DiI (DiIC18 (3)549, 551565Din itrophe nol349DiO (DiOC18(3)484, 487501, 502DiR748780LipophilicTracerDiR (DiIC18 )750779DM-NERF (high pH)497/5105

19、40Ratio Dye, pHDNP349Dopam ine340490-520DsRed558583Red fluoresce ntproteinDTAF494520DY-630-NHS621660Hemicya nelabelforprotei ns and DNADY-635-NHS634664Hemicya nelabelforprotei ns and DNAEBFP383447Enhan cedBlueFluoresce ntProteinECFP436474EnhancedCyanFluoresce ntProteinEGFP488, 498507, 516EnhaneedGre

20、enFluoresce ntProteinELF 97345530Eosin524545Erythros in529, 532554,555Erythrosi n ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrys in430540EukoLightEuropium (III) chlorideEYFP513, 520527,532Enha need Y ellowFluoresce ntProteinFast Blue360440FDA494520Feulge n (Para

21、rosa nili ne)570625FIF (Formaldehyd In duced Fluoresce nee)405433FITC490, 494520, 525FITC An tibody493517Flazo Orange375-530612Fluo-3480506, 520, 527Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluoresce in Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidi ne)361536Fluor-Ruby555582F

22、luorX494520FM 1-43479598FM 4-46515640Fura Red(high pH)572657Fura RedFluo-3Fura-2, high calcium335505Excitatio nratiodyeFura-2, low calcium363512Excitatio nratiodyeFura-2/BCECFGen acryl Brillia nt Red B520590Ge nacryl Brillia nt Y ellow 10GF430485Gen acryl Pink 3G470583Genacryl Y ellow 5GF430475Gen e

23、Blazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type,non-UV excitatio n475509(wtGFP)GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granu lar Blue355425Haematoporphyri n530-560580Hoechst 33258345487Hoechst 33342347Filter sets in stalled on the Axiovert 200M:1)BrightfieldDIC: