某營(yíng)養(yǎng)膠囊對(duì)小鼠免疫功能的影響

1、某營(yíng)養(yǎng)膠囊對(duì)小鼠免疫功能的影響 作者：周靜，陳瓊瑤，徐薇，盧潤(rùn)生，蔣中仁，金偉，謝惠萍，葛宇杰【摘要】 目的 評(píng)價(jià)某營(yíng)養(yǎng)膠囊對(duì)動(dòng)物是否具有增強(qiáng)免疫力的功能。方法 選用正常動(dòng)物，連續(xù)30天灌胃后，按照保健食品檢驗(yàn)與評(píng)價(jià)技術(shù)規(guī)范進(jìn)行試驗(yàn)。結(jié)果 經(jīng)30天灌胃后，3個(gè)劑量組動(dòng)物的抗體生成細(xì)胞數(shù)明顯增加，與陰性對(duì)照組比較差異有顯著性(P0.01，P0.05，P0.01);3個(gè)劑量組動(dòng)物的吞噬率及吞噬指數(shù)與對(duì)照組比較，也差異有顯著性(P0.05)。結(jié)論 該營(yíng)養(yǎng)膠囊對(duì)動(dòng)物具有增強(qiáng)免疫力的作用。 【關(guān)鍵詞】 小鼠;營(yíng)養(yǎng)膠囊;免疫力AbstractObjective To evaluate whether a

2、nutrient capsule could improve animals immunity or not.Methods Chose healthy animals and gave the nutrient capsule to them by gavage,then performed tests according to Technical Standards for Testing & Assessment of Health Food.Results Being gave the nutrient capsule by gavage after 30 days,the abili

3、ty of the lymphocytes proliferation of the three dosage groups were obviously enhanced. There were significant differences between these two groups and the negative control group(P0.01，P0.05，P0.01). The phagocytic percentage and the phagocytic index of the low-dosage group and the high-dosage group

4、were significantly different from the negative control group(P0.05)。2.2 某營(yíng)養(yǎng)膠囊對(duì)小鼠體液免疫的影響(見(jiàn)表2) 連續(xù)灌胃30天后，3個(gè)劑量組動(dòng)物的溶血空斑數(shù)與對(duì)照組比較差異均有顯著性(P0.01，P0.05，P0.01)。2.3 某營(yíng)養(yǎng)膠囊對(duì)單核-巨噬細(xì)胞功能的影響(見(jiàn)表3) 連續(xù)灌胃30天后，低、高劑量組動(dòng)物的吞噬率及吞噬指數(shù)與陰性對(duì)照組比較差異有顯著性(P0.05)。表1 某營(yíng)養(yǎng)膠囊對(duì)小鼠臟器系數(shù)的影響表2 某營(yíng)養(yǎng)膠囊對(duì)抗體生成細(xì)胞的影響表3 某營(yíng)養(yǎng)膠囊對(duì)小鼠巨噬細(xì)胞雞紅細(xì)胞能力的影響3 討論該營(yíng)養(yǎng)膠囊連續(xù)灌胃30天后，對(duì)小鼠的體重指數(shù)(臟器/體重)、NK細(xì)胞活性及對(duì)小鼠的細(xì)胞免疫均無(wú)影響，而對(duì)小鼠體液免疫試驗(yàn)，結(jié)果為陽(yáng)性;對(duì)小鼠的單核細(xì)胞吞噬功能試驗(yàn)結(jié)果為陽(yáng)性。表明該營(yíng)養(yǎng)膠囊對(duì)動(dòng)物具有增強(qiáng)免疫力的作用。筆者認(rèn)為3今后對(duì)其有免疫調(diào)節(jié)功能的保健食品進(jìn)行功效研究時(shí)，應(yīng)注意劑量設(shè)計(jì)和觀察周期應(yīng)符合保健食品的特點(diǎn)。保健食品的特點(diǎn)是小劑量，長(zhǎng)期服用，使其所含活性物質(zhì)發(fā)揮功效作用，與藥品的大劑量，急性作用不盡相同。目