挑戰(zhàn)杯匯報(bào)最終版

1、20種川道地中藥材對番茄灰霉病菌的抑菌效果研究Inhibitory effects of 20 kinds of Sichuan authentic herbal medicine on mycelial growth of Botrytis cinereaXue-dan SunCollege of Life and Basic Science,Sichuan Agricultural University CONTENTSBackground Experiment Information Results and Discussion Experimental Process10324304-

2、070810-152College of Life and Basic Science,Sichuan Agricultural University 1. Experiment InformationMaterialsPurposeThe active ingredients of 20 kinds of authentic herbal medicine be extracted in order to screen the significant inhibitory effect of extracting solution.Isotope research laboratory(10

3、1) Botrytis cinerea provided by the laboratory.Buying medicines from Tongren.LaboratoryCollege of Life and Basic Science,Sichuan Agricultural University HazardsTomato gray moldEtiology:Botrytis cinerea1423Chemical control Physical control Resistance breedingFlowering periodFruiting periodReproductiv

4、e capacity Hereditary variationAdaptabilityBiological control42.1 Background2.1 BackgroundCollege of Life and Basic Science,Sichuan Agricultural University Feasibility AnalysisFeasibility Analysis51.Chemical control 3.Resistance breeding2.Physical control 4.Biological control2.2 BackgroundCollege of

5、 Life and Basic Science,Sichuan Agricultural University 62. 2 BackgroundCollege of Life and Basic Science,Sichuan Agricultural University 7SamplesActive partsSamplesActive parts川 烏 Aconiti Radixparent root川木通Clematidis Armandii Caulisrattan川牛膝 Cyathulae Radixroot花 椒 Zanthoxyli Pericarpiumpericarp川楝子

6、 Toosendan Fructusfruits金錢草 Lysimachiae Herbaherb續(xù) 斷 Dipsaci Radix root川射干 Iridis Tectori Rhizomarhizome使君子 Quisqualis Fructusfruits金銀花 Lonicerae Japonicae Flosflower bud黃 連 Coptidis Rhizomarhizome石菖蒲 Acori Tatarinowii Rhizomarhizome半 夏 Pinelliae Rhizomatuber大 黃 Rhei Radix Et Rhizomaroot/rhizome澤 瀉

7、Alismatis Rhizomatuber白 芍 Paeoniae Radix Albaroot巴 豆 Crotonis Fructusfruits黃 柏 Phellodendri Chinensis Cortexbark桔 梗 Platycodonis Radix root附 子 Aconiti Lateralis Radix PraeparatarootTab1.Catalogue of Sichuan authentic herbal medicineCollege of Life and Basic Science,Sichuan Agricultural University 8D

8、rying ，Crushed samples，over 40 mesh，split chargingMaterial handling Morphological identification Antibacterial test （mycelial growth rate mycelial growth rate methodmethod）Extracting herbal medicineacetone，ethanol，ethyl acetate3. Experimental ProcessCollege of Life and Basic Science,Sichuan Agricult

9、ural University 94.1.ResultsTreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)黃 柏0.150.02a99.38續(xù) 斷4.930.65d79.52川射干0.350.15ab98.57澤 瀉10.190.47f57.63花 椒0.370.04ab98.45巴 豆11.110.63f53.81黃 連0.400.10ab98.36川牛膝11.191.24f5

10、3.48石菖蒲0.540.12ab97.74附 子14.090.81g41.42大 黃0.630.07ab97.37 金錢草14.160.27g41.13川 烏0.720.18ab97.00白 芍15.880.75h33.99金銀花1.390.35ab94.23使君子19.140.59i20.41川木通3.320.47cd86.19啶酰菌胺4.250.23d82.35桔 梗3.880.24d83.85甲基托布津1.870.11bc92.22半 夏4.130.93d82.83乙 醇25.140.31j川楝子4.460.66d81.44無菌水24.050.72jNote: “”represent

11、no Inhibitory effect，values in the column followed by different letters indicate significant differences among treatments at p0.05.Tab2.Inhibitory effects of 20 species of alcoholic extracts of Sichuan authentic herbal medicine on mycelial growth of Botrytis cinereaea. .College of Life and Basic Sci

12、ence,Sichuan Agricultural University 104.1.ResultsTab3.Inhibitory effects of 20 species of acetonic extracts of Sichuan authentic herbal medicine on mycelial growth of Botrytis cinerea.Note: “”represent no Inhibitory effect，values in the column followed by different letters indicate significant diff

13、erences among treatments at p0.05.TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)石菖蒲0.230.02a99.05 附 子19.050.24hijk21.24大 黃2.940.26b87.86桔 梗19.190.33ijk20.63川射干5.300.40c78.08川木通19.360.42ijk19.93黃 連7.150.15d70.45半

14、夏20.010.58jklm17.24花 椒10.140.30e58.07 金錢草20.140.42klm16.73黃 柏10.290.22e57.44 澤 瀉21.050.89lm12.94金銀花14.950.31f38.16白 芍21.300.79m11.94 川 烏16.840.41g30.35川牛膝23.080.71n4.56續(xù) 斷17.730.43gh26.68啶酰菌胺4.190.14c82.66 川楝子18.030.60ghi25.43甲基托布津1.780.11b92.65使君子18.670.22hij22.78丙 酮19.860.23jkl17.87巴 豆18.940.58hij

15、k21.69無菌水24.180.23nCollege of Life and Basic Science,Sichuan Agricultural University 114.1.ResultsTreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)川射干0.290.10a98.83大 黃10.110.11g59.36黃 連0.320.08a98.73金錢草10.180.30g59.

16、08桔 梗0.340.08a98.65澤 瀉12.470.34h49.88石菖蒲0.400.08a98.41川木通18.410.30i26.02川 烏0.400.10a98.41川楝子18.550.18i25.45使君子0.460.07ab98.16 白 芍18.610.45i25.20金銀花1.940.24c92.21附 子21.010.37j15.57黃 柏3.700.24d85.12川牛膝21.820.25k12.30半 夏4.040.23d83.75啶酰菌胺4.190.18de83.18花 椒4.780.24e80.79甲基托布津1.100.06b95.60續(xù) 斷6.320.25f74

17、.58乙酸乙酯23.460.33l5.69巴 豆6.610.11f73.45無菌水24.880.16mTab4.Inhibitory effects of 20 species of ethyl acetate extracts of Sichuan authentic herbal medicine on mycelial growth of Botrytis cinerea.Note: “”represent no Inhibitory effect，values in the column followed by different letters indicate significan

18、t differences among treatments at p0.05.College of Life and Basic Science,Sichuan Agricultural University 124.1.ResultsTab5.Inhibitory effects of 20 species of trichloromethane extracts of Sichuan authentic herbal medicine on mycelial growth of Botrytis cinerea.Note: “”represent no Inhibitory effect

19、，values in the column followed by different letters indicate significant differences among treatments at p0.05.TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)續(xù) 斷0.360.08a98.58巴 豆14.940.16k40.60金銀花0.360.07a98.56川楝子

20、15.610.32l37.95石菖蒲3.000.14b88.09桔 梗16.190.23lm35.65半 夏4.580.20c81.81附 子16.760.21m33.38黃 連5.330.26d78.80使君子20.060.21n20.23川射干6.330.18e74.84川 烏22.050.17o12.33 黃 柏7.450.14f70.39川木通22.400.29op10.93川牛膝8.320.18g66.94金錢草22.690.32p9.79 大 黃10.440.20h58.50啶酰菌胺5.300.10d78.95澤 瀉13.070.18i48.05甲基托布津3.020.20b88.0

21、1花 椒13.370.20ij46.85三氯甲烷22.900.28p8.94白 芍13.730.15j45.39無菌水25.150.28qCollege of Life and Basic Science,Sichuan Agricultural University 134.1 ResultsTab6.Inhibitory effects of 20 species of petroleum ether extracts of Sichuan authentic herbal medicine on mycelial growth of Botrytis cinerea.Note: “”re

22、present no Inhibitory effect，values in the column followed by different letters indicate significant differences among treatments at p0.05.TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)石菖蒲5.540.13a79.04金錢草22.990.

23、10g13.05川楝子16.820.12b36.39川射干23.100.35g12.65金銀花18.190.27c31.20花 椒23.650.14h10.56大 黃18.560.14c29.80川牛膝24.330.21i7.99黃 柏19.590.16d25.91使君子24.530.20ij7.22 巴 豆19.840.21d24.97半 夏24.770.11ij6.33白 芍21.150.15e20.01續(xù) 斷24.930.07j5.72川 烏21.660.14ef18.10澤 瀉25.710.21k2.77黃 連21.760.30f17.72啶酰菌胺5.320.21a79.87桔 梗22

24、.530.11g14.79甲基托布津3.610.21l86.33川木通22.540.13g14.77 石油醚25.720.26k2.74附 子22.850.25g13.60 無菌水26.440.19mCollege of Life and Basic Science,Sichuan Agricultural University 144.1 ResultsTab7.Inhibitory effects of 20 species of aqueous extracts of Sichuan authentic herbal medicine on mycelial growth of Botr

25、ytis cinerea.Note: “”represent no Inhibitory effect，values in the column followed by different letters indicate significant differences among treatments at p0.05.TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)TreatmentsInhibition zone diameter (mm)(meanSEM)Inhibitory rate (%)黃 連6

26、.120.13a76.30續(xù) 斷22.010.26j14.71澤 瀉7.040.24b72.70桔 梗23.080.25k10.55川 烏7.810.25c69.75川木通23.900.10l7.38附 子11.660.22d54.80白 芍24.450.25l5.26金銀花12.090.17d53.13石菖蒲24.590.26l4.69金錢草13.960.14e45.90川牛膝25.700.28m0.39大 黃14.040.26e 45.58使君子26.050.36m-0.97 黃 柏14.880.33f42.35巴 豆26.360.33m-2.16 川射干15.680.03g39.23啶酰

27、菌胺5.260.15n79.62半 夏15.940.39g38.21甲基托布津3.050.18o88.20花 椒19.940.17h22.72無菌水25.800.4m 川楝子20.770.29i19.52 College of Life and Basic Science,Sichuan Agricultural University 15Conclution1 (1) A.T. ,C.R. , I.T. , A.R ,D.R. , R.R , R.C. ,P.R. and Z.P. has a significant inhibitory effect . (2)Ethanol，ethyl acetate extract