…AMINOACIDMETABOLISM：12……氨基酸代謝

1、.12 AMINO ACID METABOLISMThe goals for this exercise are:to provide practice in selecting, performing, and interpreting biochemical teststo demonstrate the differences in test results that are observed for different species of bacteria of a similarGram reaction, morphology, and arrangementto connect

2、 the discussion of metabolism in lecture with the tests and results observed in labto provide practice in using test results for the purpose of identifying bacteriaInstructions:1. Use the Tutorial available upon entering the software and refer to Exercises 1-4 in this Basic Training Manual for VUMic

3、ro 3.11 manual to learn the basics of performing biochemical tests. The reference library (explained in Exercise 2), your textbook, and your lab manual will provide additional information on the tests, media, and reagents required to complete this assignment.2. Enter the VirtualUnknown Microbiologys

4、 Virtual Lab and select Media Tests 1 from the list of predefined unknowns. This organism is Escherichia coli. DO NOT check the box permitting autoinoculation3. Complete the tests listed in the table below, recording the results for E. coli. They are automatically recorded in the Virtual Laboratory

5、Report as well.a. Arginine dihydrolase test, lysine decarboxylase test, and ornithine decarboxylase test use similar media and are performed in the same manner.b. Phenylalanine deaminase test uses a different type of medium and is performed in a different manner.4. Repeat the above assignment for th

6、e different microbes assigned for each of the following TextbookExercises:Media Tests 2: Enterobacter aerogenes; Media Tests 3: Salmonella typhi; and Media Tests 4: Proteus vulgaris.Fill in the table with the results for all four microbes. Print out and attach the Virtual Laboratory Reports for each

7、 of the four microbes:TestE. coliMedia Tests 1Enterobacter aerogenesMedia Tests 2Salmonella typhiMedia Tests 3Proteus vulgarisMedia Tests 4Arginine dihydrolase test:Lysine decarboxylase test:Ornithine decarboxylase test:Phenylalanine deaminase test:51:Basic Training Manual for VUMicro 3.11Name:_ Cop

8、yright © 2003-2005, Intuitive Systems, Inc.Section:_ Below are some questions relating to the amino acid tests used for bacterial identification:5. What ingredient is responsible for the initial color of lysine decarboxylase broth?6. How does this ingredient allow detection of the metabolism of

9、 lysine by the microbe?7. Why must this test be observed at 24 and 48 hours?8. Why must there be a color change at 24 hours in order for results at 48 hours to be valid?9. Indicate your observations for lysine decarboxylase broth during the lysine decarboxylase test:Color of mediumpH of mediumUninoc

10、ulated brothObservation at 24 hoursObservation at 48 hours52Basic Training Manual for VUMicro 3.11Name:_ Copyright © 2003-2005, Intuitive Systems, Inc.Section:_ 10. Based on the results in the table above, what sequence of events must take place before the amino acid in each medium is metaboliz

11、ed?12. In contrast to the decarboxylase and dihydrolase media just used, phenylalanine agar for the phenylalanine deaminase test is similar in appearance to routine culture media like nutrient agar. How can this medium without a colored indicator be useful for detecting metabolism of phenylalanine?13. Why must reagents be added to the phenylalanine agar slant after,