北大神經(jīng)生物學(xué)2緒論P(yáng)PT學(xué)習(xí)教案

1、會計(jì)學(xué)1北大神經(jīng)生物學(xué)北大神經(jīng)生物學(xué)2緒論緒論Day of treatment1234567891060708090100110120130Control2 Hz2/100 Hz100 Hzn=13-16*Heart Rate (beat/min)第1頁/共36頁Body Weight (kg)01234567891011Days of Treatment45505560Control2Hz2/100Hz100Hz(n=26-32)Effect of HANS on Body Weight during Opiate Withdrawal*第2頁/共36頁第3頁/共36頁CRAVING: th

2、e most difficult issue一朝吸毒，終身想毒。第4頁/共36頁第5頁/共36頁The auto-track conditioned place preference (CPP) instrument第6頁/共36頁Morphine-induced conditioned place preference (CPP)Dose-dependent effect00.01 0.03 0.10.31.03.0 10.00.000.250.500.75* *n=9-12Mor ( mg/kg )*p0.05, *p0.01, *p 100 Hz100 Hz 2 Hz第13頁/共36頁E

3、lectroacupuncture treatment for drug addictionVTAOpiateGABANAcDADATCocaineEuphoriaEnkDynElectroacupuncture2 Hz100 HzPresynaptic inhibitionSubstitution第14頁/共36頁A100 bp2000 bp1000 bp750 bp500 bp250 bpDL 2000 NSCPP2 Hz100 HzrestrainGAPDH (532 bp)PPD (250 bp)Effect of 100Hz EA on the level of PPD mRNA i

4、n NAc of CPP rats B0.00.51.01.5Grey scale ratios(PPD/GAPDH)Treatment*#n=4NSCPP2 Hz100 Hzrestrain第15頁/共36頁Effect of 2Hz EA on the level of PPE mRNA in NAc of CPP rats Grey scale ratios(PPE/GAPDH)0.000.250.500.751.00NSCPP*#2 Hz100 Hzrestrainn=42000 bp1000 bp750 bp500 bp250 bp100 bpDL2000CPP2 Hz100 Hzr

5、estrainGAPDH（532 bp）PPE（402 bp）NS第16頁/共36頁美國權(quán)威性美國權(quán)威性“物質(zhì)濫用物質(zhì)濫用”教科書教科書Joyce Lowinson 等主編等主編第第4版，版，2004“針刺戒毒針刺戒毒”一章一章由韓濟(jì)生編寫由韓濟(jì)生編寫第17頁/共36頁肥肥 胖胖 癥癥能量代謝失衡能量代謝失衡對美食的渴求對美食的渴求藥物成癮藥物成癮身體依賴：戒斷癥狀身體依賴：戒斷癥狀精神依賴：心癮精神依賴：心癮痛與鎮(zhèn)痛痛與鎮(zhèn)痛感知功能感知功能情緒反應(yīng)情緒反應(yīng)第18頁/共36頁肥胖的發(fā)展趨勢肥胖的發(fā)展趨勢第19頁/共36頁肥胖發(fā)病機(jī)理肥胖發(fā)病機(jī)理GenesEnvironmental factor

6、s 第20頁/共36頁048121620242832-4-3-2-10 A HANS B C HANS Dn=16Time ( week )ABCD-4-3-2-10*HANS ( 3 time / week )Change of body weight ( kg )梁門，一對。天樞，一對。水道，一對。梁丘，公孫。頻率：2/100 Hz每周3次每次30 分鐘第21頁/共36頁02468101214160100200300400500600700High energy diet (n =80)Normal diet (n =20)*Time (week)Body weight ( g )Cont

7、rolDRDIO0100200300400500600700800*#High energy dietBody weight ( g )* P0.001 vs. control and DR; # P0.05 vs. Control第22頁/共36頁促進(jìn)攝食和抑制攝食的神經(jīng)肽促進(jìn)攝食 feedingNPY, Orexin,AGRP, MCH抑制攝食 feedingLeptin, CART, a-MSH, CRHNPYOrexinAGRPMCHCARTLeptin -MSH Insulin瘦胖ThinObese第23頁/共36頁100Hz036912151821242730Time ( day

8、)9092949698100102104106108110Body weight ( % )DIO大鼠實(shí)驗(yàn)結(jié)果（體重大鼠實(shí)驗(yàn)結(jié)果（體重%）Restrain2Hz第24頁/共36頁* P0.001 vs. DIO; # p0.01, # P0.001 vs. Restriction-30-20-100102030405060*# Body weight ( g )DIORestrain100 Hz2 Hz第25頁/共36頁大鼠實(shí)驗(yàn)結(jié)果（大鼠實(shí)驗(yàn)結(jié)果（ACTHACTH）Normal DR DIO Restrain 100Hz EA 2Hz EA051015202530*High energy di

9、et*Plasma ACTH (ng / ml)* P0.05 vs. Normal, DR and DIO.第26頁/共36頁NormalDRDIORestriction100Hz EA2Hz EA020406080100120140160High energy diet*#Energy intake ( kcal / day )* P0.001 vs. other groups; # P0.001 vs. 2Hz and 100 Hz EA group 第27頁/共36頁 CART -MSH第28頁/共36頁ControlDiet induced ObesityDiet Resistant

10、Restrain100Hz2HzCART immunoreactivity in hypothalamus第29頁/共36頁ControlDiet induced ObesityDiet ResistantRestrain100 Hz 2Hz-MSH immunoreactivity in hypothalamus第30頁/共36頁 n = 5NormalDRDIORestrain100Hz EA0255075100125 *High energy diet CART positive cells in ARCHigh energy dietNormalDRDIORestrain100Hz EA2Hz EA010203040506070* a-MSH positive cells in ARC *2Hz EACART-MSH第31頁/共36頁 國際神經(jīng)科學(xué)神經(jīng)科學(xué)領(lǐng)域：英國TiNS雜志（2003） 國際針灸針灸領(lǐng)域：德國SEIRIN針灸獎（2002） 國際戒毒戒毒理論：美國“物質(zhì)成癮”經(jīng)典教科書 臨床戒毒戒毒實(shí)踐：韓氏戒毒一年以上不復(fù)吸20例36年針灸研究，12年戒毒研究所獲成績第32頁/共36頁Trends in Neuroscience, 2003; 26: 17-22 第33