乳酸脫氫酶（LDHL）液體試劑盒說明書

1、Lactate Dehydrogenase Assay Kit (LDH-L)LP Cat. 2050: R1 (60ml3) + R2 (54ml1)2550: R1 (80ml3) + R2 (72ml1)8050: R1 (45ml30 + R2 (40ml1)Intended Use:For the quantitative determination of LDH-L in human serumAssay Principle:LDHL-lactic acid + NAD+ Pyruvic acid + NADH + H+Reagents Composition:ReagentCom

2、ponents ConcentrationR1 Tris buffer（）KClL-Lithium lactate100mmol/L 100mmol/L 52mmol/LR2 NAD/LStability:12 months at 28, protecting from light.Sample:Fresh Serum，heparinized plasma. Note: Strictly avoid using hemolysis sample. Assay Conditions:Wavelength：340nmTemperature：37Cuvette：1cmProcedures:Ready

3、 For UseBlankSampleSample24lWater24lR1900l900lMix，stand at the appropriate temperature for 5 minutesR2 270l270lMix，stand at the appropriate temperature for 1 minutes，read the absorbance A for 1 minute.Calculation:LDH-L(U/L) = (Asample/minAcontrol/min)F VtF = 1000 = 7897 VsKVt = reaction volume, Vs =

4、 sample volumeNADPH under 340nm wavelength KReference value:109245U/LNote: The reference range should be determined by each hospital to confirm with the characteristics of the region being tested.Linearity:The test is linear up to 1000U/L.Precautions and warnings:1. When A/min0.130, dilute the sampl

5、e with 0.9% NaCl and re-assay it.2. Discard the kit when the Blank Absorbance is over 0.6.3. Sodium Azide included. Avoid ingestion or contact with skin. In case of skin contact, flush with copious amounts of water and wash with soap and water for at least 15 minutes. If swallowed, wash mouth with w

6、ater provided person is conscious. Call a physician or poison control.4. Shall be stored at 28. This product is stable until the expiration date indicated on the label under the specified storage conditions.Bibliography:1. Bergmeyer: Methods of Enzymatic Analysis. Third Edition, Vol.:126132Parameter

7、 when applied on Hitachi analyzers:717070607150Assay CodeRATE-ARATE-ARATE-AReaction time10 10Assay Point222821273040Wavelength405/340405/340405/340Sample Volume577R1 Volume200250250R2 Volume75R3 Volume6075R4 Volume250002500025000INC/DECINCINCINCLinearLinearK Factor1100SD LimitDuplicate Limit50050050

8、0SI ABS Limit-5005000-5005000UnitU/LU/LU/LSTD(1)Conc000STD(2)Conc000Expected Value109245109245109245K Factor841375287528Registration number: 3400102-2004Executed Standard: YZB/GB 2082-2003Biosino Bio-Technology and Science Inc. Add：No.27, Chaoqian Road, Changping District, 102200 Beijing, ChinaTel：+

9、86-10-69722840Fax：+86-10-69722840Website：n ParametersHITACHI 70201 TESTLDH-L2 METHODRATE A4 REACTION TIME105 MEASURING POINT 1226 MEASURING POINT 2289 WAVELENGTH-240510 WAVELENGTH-134011 SAMPLE VOL.512 REAGENT 1 VOL.20015 REAGENT 2 VOL.018 REAGENT 3 VOL.6021 CAL. TYPEK FACTOR23 CAL.1 CONC.024 CAL.1

10、POS.9925 CAL.2 CONC.-36 K FACTOR841345 DUPLICATE LIMIT50049 ABS. LIMIT2500050 REACTION DIR.(I/D)I56 INSTRUMENT CONSTANT a57 INSTRUMENT CONSTANT b0OLYMPUS AU4006006405608001000TESTLDH-LLDH-LSAMPLE VOL.DILUTION5070R1 REAGENT VOL.DILUTIONR2 REAGENT VOL.DILUTION20006002500750WAVELENGTH-1WAVELENGTH-2340410340410METHODRATERATEREACTION SLOPE+MEASURING POINT 1:FIRST MEASURING POINT 1:LAST 15211116NO LAG-TIMEYES