Xevo G2 QT培訓(xùn)資料

1、2010 Waters Corporation | COMPANY CONFIDENTIAL42089_12010 Waters Corporation | COMPANY CONFIDENTIAL42089_1OverviewXevo G2 QTOf MS OpticsDescriptionVacuum systemInstrument communicationStatus panel LEDsRear panel connectionsExternal EPC connectionsRebooting EPC & ElectronicsSource waste trap bott

2、leMS ConsoleOverviewIntellistartOverview (Normal Mode)Use Calibration Profile Calibration CheckLock Mass CheckDetector CheckLCMS System Check Overview (Configuration Mode) Calibration Profile EditorLock Mass EditorReference File EditorCreate CalibrationAssisted CalibrationManual CalibrationLockSpray

3、 SetupDetector SetupInteractive FluidicsOverviewMS Method editorData processingOpening data filesBasic data processingSmoothSubtractAutomatic Peak DetectionTune Page (Normal view)Tune Page (System settings)Advanced featuresQuad profile theoryQuad profilesoManual fixed profileoManual profileoDwell &a

4、mp; ramp time oAuto profileTarget EnhancementResolutionInlet editornanoACQUITY installationInlet method editor2010 Waters Corporation | COMPANY CONFIDENTIAL42089_12010 Waters Corporation | COMPANY CONFIDENTIAL 442089_12010 Waters Corporation | COMPANY CONFIDENTIAL 542089_1 Xevo G2 QTof MS has 2 mode

5、s of operation in the TOF analyser:Sensitivity modeResolution mode 2010 Waters Corporation | COMPANY CONFIDENTIAL 642089_1Functional specification TOF mass rangeo Sensitivity Mode100,000o Resolution Mode100,000 Maximum acquisition rate (TOF mode)o 30 scans/sec (MS or MS/MS) 0.019s Scan and 0.014s IS

6、D, mass range 100 to 2000 Dao DRE has maximum scan rate of 15 scans/sec Automatic Interscan Delay (ISD)o 100 ms LockSpray datao 14 ms for all other data2010 Waters Corporation | COMPANY CONFIDENTIAL 742089_1 The system is backed by either a Scroll (dry) or Rotary (wet) pump both require annual maint

7、enance and the rotary may require oil top-ups throughout the year. There are 3 Turbo pumps, one for each pumping region Source Analyser TOF Vacuum readbacks created from Penning gauge (TOF) Pirani gauges (Source and Collision Cell)2010 Waters Corporation | COMPANY CONFIDENTIAL 842089_1Waters DHCP Se

8、rver The EPC acts as a DHCP Client and the Host PC as the DHCP Server. Waters provides a service called “Waters DHCP Server” which is loaded on the Host PC during the MassLynx installation process.The only requirement is that the Instrument LAN card is set with the Static IP-address 192.168.0.1Ping

9、EPC to test basic communications Click Start Run and type “ping epc t”. (A packet of data is sent from the host PC to the EPC requesting a reply).2010 Waters Corporation | COMPANY CONFIDENTIAL 942089_12010 Waters Corporation | COMPANY CONFIDENTIAL 1042089_12010 Waters Corporation | COMPANY CONFIDENT

10、IAL 1142089_12010 Waters Corporation | COMPANY CONFIDENTIAL 1242089_1Pump override switchMaintenance switch2 methods for EPC reboot:EPC reset switch on the EPCTyping “reboot” at telnet epc prompt if communication to EPC is still presentNOTE: Pump override switch can remain in AUTO (Up) positionElect

11、ronics reboot:Put Pump override switch into OVERRIDE (Down) position.Put the Maintenance switch into the OFF (Down) position.Switch the power off to the EPC.Wait for 30 seconds then put the Maintenance switch into the ON (Up) position.Switch on the power to the EPC.Wait for communications to be esta

12、blished.Open MassLynx and ensure vacuum is ok before putting Pump override switch back to AUTO (Up) position.2010 Waters Corporation | COMPANY CONFIDENTIAL 1342089_1Instrument exhaustOutlet to lab exhaustPilot valve2010 Waters Corporation | COMPANY CONFIDENTIAL42089_12010 Waters Corporation | COMPAN

13、Y CONFIDENTIAL42089_1Step 1Select the Shortcut tab Step 2Select the instrument tabStep 3Click MS Console to open the Console window 2010 Waters Corporation | COMPANY CONFIDENTIAL 1642089_1Instruments are displayed here, with their respective control pages2010 Waters Corporation | COMPANY CONFIDENTIA

14、L 1742089_1Drop down functions2010 Waters Corporation | COMPANY CONFIDENTIAL 1842089_1Instrument Status2010 Waters Corporation | COMPANY CONFIDENTIAL 1942089_1Source Standby2010 Waters Corporation | COMPANY CONFIDENTIAL 2042089_1MS Operate2010 Waters Corporation | COMPANY CONFIDENTIAL 2142089_1Launc

15、h the MS Tune page2010 Waters Corporation | COMPANY CONFIDENTIAL 2242089_1Pause/Run MS console plots 2010 Waters Corporation | COMPANY CONFIDENTIAL 2342089_1Ion mode and analyser mode information2010 Waters Corporation | COMPANY CONFIDENTIAL 2442089_1Current tune page and calibration information2010

16、 Waters Corporation | COMPANY CONFIDENTIAL 2542089_1Temperature and gas flow statuses2010 Waters Corporation | COMPANY CONFIDENTIAL 2642089_1Readback plots2010 Waters Corporation | COMPANY CONFIDENTIAL 2742089_1Should the Status LED be RED, the problem will be identified here, with suggested actions

17、 to resolve.2010 Waters Corporation | COMPANY CONFIDENTIAL 2842089_1Operate or Standby2010 Waters Corporation | COMPANY CONFIDENTIAL 2942089_1Nebuliser, Cone & Desolvation gas (N2 ) on/off control2010 Waters Corporation | COMPANY CONFIDENTIAL 3042089_1Collision gas (Ar ) on/off control2010 Water

18、s Corporation | COMPANY CONFIDENTIAL 3142089_1Sample FluidicsReference Fluidics2010 Waters Corporation | COMPANY CONFIDENTIAL 3242089_1Sample FluidicsReference FluidicsRefill the syringe to the maximum volume2010 Waters Corporation | COMPANY CONFIDENTIAL 3342089_1Sample FluidicsReference FluidicsPur

19、ges the syringe with the current selected vial2010 Waters Corporation | COMPANY CONFIDENTIAL 3442089_1Sample FluidicsReference FluidicsWashes the syringe with wash solvent2010 Waters Corporation | COMPANY CONFIDENTIAL 3542089_1Sample FluidicsReference FluidicsPurges with the wash solvent and fills t

20、he syringe with select sample vialOverview of the fluidics is later in this presentation2010 Waters Corporation | COMPANY CONFIDENTIAL 3642089_1Reinitialise MS Console2010 Waters Corporation | COMPANY CONFIDENTIAL 3742089_1Information only2010 Waters Corporation | COMPANY CONFIDENTIAL 3842089_1By de

21、selecting the status reports it will also disable the monitoring/check of that feature. Warning: Overriding a feature that contributes to the conformation of a fully functional system may cause future problems.2010 Waters Corporation | COMPANY CONFIDENTIAL 3942089_1You can keep an electronic record

22、of any tasks you complete e.g.Sample Cone cleaned 26/01/09.Fresh Leucine added to Vial B.2010 Waters Corporation | COMPANY CONFIDENTIAL 4042089_1This shows your Input & Output Events at the rear of the Xevo G2 QTof MS2010 Waters Corporation | COMPANY CONFIDENTIAL 4142089_1This allows the user/FS

23、E to input Service details (in normal mode). Click on View History to see previous service details.2010 Waters Corporation | COMPANY CONFIDENTIAL 4242089_1This saves the various Plots, for the Xevo G2 QTof MS, which continuously display the performance of the listed parameters. 2010 Waters Corporati

24、on | COMPANY CONFIDENTIAL 4342089_1This prints the various Plots, for the Xevo G2 QTof MS, which continuously display the performance of the listed parameters.2010 Waters Corporation | COMPANY CONFIDENTIAL 4442089_1This activates instrument reset features. For FSE use only.2010 Waters Corporation |

25、COMPANY CONFIDENTIAL 4542089_1Collates files from host PC to a Zip file, which can be sent to Waters Support personnel.2010 Waters Corporation | COMPANY CONFIDENTIAL 4642089_1Settings are defined by the customer to determine what is sent to Waters Support Personnel. Collates files from host PC to a

26、Zip file, and automatically sends them to Waters Support personnel.2010 Waters Corporation | COMPANY CONFIDENTIAL42089_1PrepareAnalyseInterpretDecide2010 Waters Corporation | COMPANY CONFIDENTIAL 4842089_1Recommended for basic level users to carry out instrument checks to ensure performance of the i

27、nstrument is being maintained.2010 Waters Corporation | COMPANY CONFIDENTIAL 4942089_1This will load a previously created Calibration Profile2010 Waters Corporation | COMPANY CONFIDENTIAL 5042089_1This will launch a wizard to check any previously defined Calibration Profile2010 Waters Corporation |

28、COMPANY CONFIDENTIAL 5142089_1This will launch a wizard to check a previously defined LockMass profile (with source settings) is still acceptable2010 Waters Corporation | COMPANY CONFIDENTIAL 5242089_1This will launch a wizard to check the Ion area is acceptable at the current Detector voltage2010 W

29、aters Corporation | COMPANY CONFIDENTIAL 5342089_1This will launch your LCMS System check, to ensure your instrument is performing optimally2010 Waters Corporation | COMPANY CONFIDENTIAL 5442089_1Click Start to run the wizard for the chosen activityOnly select one Check at a time2010 Waters Corporat

30、ion | COMPANY CONFIDENTIAL 5542089_1Once you have run the appropriate wizard and clicked Start a progress bar will update you on the status.The updates will vary depending upon the wizard chosen.2010 Waters Corporation | COMPANY CONFIDENTIAL 5642089_1Tick the Use Calibration Profile box and the clic

31、k Start to run the wizard to load the calibration Profile2010 Waters Corporation | COMPANY CONFIDENTIAL42089_1From the pull down menu only profiles that are available (green tick next to the the profile) can be selected. Profiles with orange triangle are not available to use.2010 Waters Corporation

32、| COMPANY CONFIDENTIAL42089_1Only calibrated profiles can be selected2010 Waters Corporation | COMPANY CONFIDENTIAL42089_1The Progress Bar gives a real time update and a green tick will appear once completed and successful2010 Waters Corporation | COMPANY CONFIDENTIAL 6042089_1A Calibration check ca

33、n only be run after a calibration setup has been successfully completed2010 Waters Corporation | COMPANY CONFIDENTIAL 6142089_12010 Waters Corporation | COMPANY CONFIDENTIAL 6242089_1Select a Calibration Profile Available for use from the drop downThis will then populate the mass range and polaritie

34、s2010 Waters Corporation | COMPANY CONFIDENTIAL 6342089_1Select the mode(s) in which to perform the calibration check2010 Waters Corporation | COMPANY CONFIDENTIAL 6442089_1This will display a non-interactive report in a web browser after the processing of the data2010 Waters Corporation | COMPANY C

35、ONFIDENTIAL 6542089_1This loads the calibration once the check has completed to ensure future acquisitions performed will use this calibration profile.2010 Waters Corporation | COMPANY CONFIDENTIAL 6642089_1Check can be made with or without LockMass correctionLockSpray fluidics options need to be se

36、lected if using LockMass. If using an alternative pump for LockMass, select Tune Page otherwise Automatic is the recommended option.2010 Waters Corporation | COMPANY CONFIDENTIAL 6742089_1Enter a suitable acceptance criteria for the calibration check2010 Waters Corporation | COMPANY CONFIDENTIAL 684

37、2089_1Click Start to run the Calibration check2010 Waters Corporation | COMPANY CONFIDENTIAL 6942089_12010 Waters Corporation | COMPANY CONFIDENTIAL 7042089_1Lock Mass check can only be run after a LockSpray Source setup has been successfully completed2010 Waters Corporation | COMPANY CONFIDENTIAL 7

38、142089_12010 Waters Corporation | COMPANY CONFIDENTIAL 7242089_1Select the LockMass Compound from the drop down menu2010 Waters Corporation | COMPANY CONFIDENTIAL 7342089_1Select a LockMass compound2010 Waters Corporation | COMPANY CONFIDENTIAL 7442089_1To progress the source conditions must have be

39、en setup for this compound2010 Waters Corporation | COMPANY CONFIDENTIAL 7542089_1If not, a Caution sign will appear in place of the green tick and stop you progressing2010 Waters Corporation | COMPANY CONFIDENTIAL 7642089_1A non-interactive report will be displayed in a web browser once the check h

40、as completed2010 Waters Corporation | COMPANY CONFIDENTIAL 7742089_1Click Start to run the LockSpray check2010 Waters Corporation | COMPANY CONFIDENTIAL 7842089_12010 Waters Corporation | COMPANY CONFIDENTIAL 7942089_1Detector check can only be run after a Detector setup has been successfully comple

41、ted NOTE: Only checks the ion area at the current detector voltage2010 Waters Corporation | COMPANY CONFIDENTIAL 8042089_12010 Waters Corporation | COMPANY CONFIDENTIAL 8142089_1Choose the reference compound (Same one used when initially setup)Ensure correct mass is selected and user can specify the

42、 polarity in which to checkA non-interactive report will be displayed in a web browser once the setup is complete2010 Waters Corporation | COMPANY CONFIDENTIAL 8242089_1Choose how to control the fluidics2010 Waters Corporation | COMPANY CONFIDENTIAL 8342089_1Click Start to run the detector check2010

43、 Waters Corporation | COMPANY CONFIDENTIAL 8442089_12010 Waters Corporation | COMPANY CONFIDENTIAL 8542089_1The system check process (LC/MS System Check) uses predefined methods that enable the system to inject diagnostic samples and identify any problems that involve the configured system by provid

44、ing data on sensitivity (signal-to-noise ratio), response (peak area, peak height), and chromatographic performance (peak width, retention time). Run the LC/MS system check periodically to acquire benchmarks of your systems performance. 2010 Waters Corporation | COMPANY CONFIDENTIAL 8642089_1LCMS Sy

45、stem Check is capable of assessing the performance of masses and retention times within an LCMS runBasic View User 2010 Waters Corporation | COMPANY CONFIDENTIAL 8742089_1Select your predefined method from the drop down2010 Waters Corporation | COMPANY CONFIDENTIAL 8842089_1Select whether a pre-run

46、is required and the vial location of your standard2010 Waters Corporation | COMPANY CONFIDENTIAL 8942089_1Setup an automatic schedule if required2010 Waters Corporation | COMPANY CONFIDENTIAL 9042089_1Set how often you would like your LCMS Check to run, remembering that you must have a vial loaded w

47、ith your sample2010 Waters Corporation | COMPANY CONFIDENTIAL 9142089_1Advance ViewHow many times you want to inject2010 Waters Corporation | COMPANY CONFIDENTIAL 9242089_1Injection volumeAdvance View2010 Waters Corporation | COMPANY CONFIDENTIAL 9342089_1Load your method filesAdvance View2010 Water

48、s Corporation | COMPANY CONFIDENTIAL 9442089_1Advance ViewSpecify your QC mass with an acceptable error2010 Waters Corporation | COMPANY CONFIDENTIAL 9542089_1Advance ViewSpecify your LC parameters and acceptable tolerance2010 Waters Corporation | COMPANY CONFIDENTIAL 9642089_1Advance ViewSwitch bac

49、k to Basic View once you have defined and tested your LCMS Check Method2010 Waters Corporation | COMPANY CONFIDENTIAL42089_1Recommended for advanced users to carry out instrument setup procedures if any checks in Normal Mode fail.2010 Waters Corporation | COMPANY CONFIDENTIAL42089_1This will launch

50、a wizard to assist with the Calibration of the instrument2010 Waters Corporation | COMPANY CONFIDENTIAL42089_1This will launch a wizard to assist you with the LockSpray Source setup of the instrument2010 Waters Corporation | COMPANY CONFIDENTIAL42089_1This will launch a wizard to assist with the Det

51、ector setup of the instrument2010 Waters Corporation | COMPANY CONFIDENTIAL42089_1Click Start to run the wizard for the chosen activity2010 Waters Corporation | COMPANY CONFIDENTIAL42089_12010 Waters Corporation | COMPANY CONFIDENTIAL42089_1User is able to Open a new Calibration Profile Editor page2

52、010 Waters Corporation | COMPANY CONFIDENTIAL42089_1User is able to edit existing Calibration Profiles, reset the Active Status or Delete the highlighted Calibration Profile2010 Waters Corporation | COMPANY CONFIDENTIAL 10542089_1Enter a Name for your Calibration Profile2010 Waters Corporation | COM

53、PANY CONFIDENTIAL 10642089_1Define your Mass Range2010 Waters Corporation | COMPANY CONFIDENTIAL 10742089_1Use a standard Mass Range for your Low and High Mass Range limits.Or customise your Low and High Mass Range limits.2010 Waters Corporation | COMPANY CONFIDENTIAL 10842089_1Decide whether you wi

54、sh the software to calibrate or whether you wish to have some input to peak matching etcNOTE:Manual Calibration is covered later2010 Waters Corporation | COMPANY CONFIDENTIAL 10942089_1Assign a reference file for Positive ion or Negative ion or Both2010 Waters Corporation | COMPANY CONFIDENTIAL 1104

55、2089_1Select the appropriate reference compound for the calibration sample that is to be infused2010 Waters Corporation | COMPANY CONFIDENTIAL 11142089_1Status now shows the profile as defined2010 Waters Corporation | COMPANY CONFIDENTIAL 11242089_1Click OK2010 Waters Corporation | COMPANY CONFIDENT

56、IAL 11342089_1Your new profile appears in the list2010 Waters Corporation | COMPANY CONFIDENTIAL 11442089_12010 Waters Corporation | COMPANY CONFIDENTIAL 11542089_1From here you will be able to create a New Lock Mass profile2010 Waters Corporation | COMPANY CONFIDENTIAL 11642089_1From here you will

57、be able to edit an existing Lock Mass profile2010 Waters Corporation | COMPANY CONFIDENTIAL 11742089_1Enter the Name of the new Lock Mass profile2010 Waters Corporation | COMPANY CONFIDENTIAL 11842089_1Select to use an existing reference file to assist with the creation of your Lock Mass file2010 Wa

58、ters Corporation | COMPANY CONFIDENTIAL 11942089_1Choose the existing Reference file from the drop down list that is required for the Lock Mass profile2010 Waters Corporation | COMPANY CONFIDENTIAL 12042089_1Select this option if the appropriate reference file does not exist for the Lock Mass2010 Wa

59、ters Corporation | COMPANY CONFIDENTIAL 12142089_1Select the Mode in which the Lock Mass will be used in experimentsNOTE: The Lock Mass mode must match the mode to be used for the acquisition 2010 Waters Corporation | COMPANY CONFIDENTIAL 12242089_1If you only wish to use one polarity in MS Mode, on

60、ly tick the masses of that polarity.In MSMS mode delete the Set Mass of the polarity that is not required2010 Waters Corporation | COMPANY CONFIDENTIAL 12342089_1Select the masses required from your reference list2010 Waters Corporation | COMPANY CONFIDENTIAL 12442089_1If a reference file isnt used as a basis for the Loc