托珠單抗作用機(jī)制 - Medchemexpress - MCE中國(guó)

1、Product Data SheetTocilizumabCat. No.: HY-P9917作靶點(diǎn): Interleukin Related作通路: Immunology/Inflammation儲(chǔ)存式: Please store the product under the recommended conditions in the COA.BIOLOGICAL ACTIVITY物活性 Tocilizumab (Anti-Human IL6R, Humanized Antibody) 種抗細(xì)胞介素-6 受體 (IL-6R) 的中和抗體，可阻 IL-6 與 IL-6R 的結(jié)合，從抑制經(jīng)典和反式

2、信號(hào)。Tocilizumab (Anti-Human IL6R, Humanized Antibody) 可于治療類風(fēng)濕性關(guān)節(jié)炎。IC & Target IC50: IL-6R1體外研究Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1-100 ng/mL; 24 hours, 48 hours) inhibits the cell proliferation(growth rate) in a dose-dependent manner, growth rate by HACM is reduced almost completely b

3、y 100 ng/mLtocilizumab2.Tocilizumab (Anti-Human IL6R, Humanized Antibody) (0.001-10,000 g/ml; 2 days) inhibits Ba/F3-gp130-IL-6R cellproliferation stimulated with hIL-6 with an IC50 of 13.5 ng/ml1.Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1 M; 6 hours) reduces the phosphorylation of STAT3 i

4、n vitroconsistent with in vivo SAS cells3.Tocilizumab (Anti-Human IL6R, Humanized Antibody) (1-5 M; 24 hours) significantly reduces VEGF mRNA expressionin SAS cells with serum-free medium3.Cell Proliferation Assay2Cell Line: Human-derived HARA-B cellsConcentration: 1 ng/mL, 10 ng/mL, and 100 ng/mLIn

5、cubation Time: 24 hours, 48 hoursResult: Inhibited the IL-6R expressed on human-derived HARA-B cells.Cell Cytotoxicity Assay1Cell Line: Ba/F3-gp130-IL-6R cellsConcentration: 0.00110000 g/mlIncubation Time: 2 daysResult: Inhibited Ba/F3-gp130-IL-6R cell proliferation.Page 1 of 2 www.MedChemEWestern B

6、lot Analysis3Cell Line: SAS cellsConcentration: 1 MIncubation Time: 6 hoursResult: Reduced the phosphorylation of STAT3.RT-PCR3Cell Line: SAS cellsConcentration: 1-5 MIncubation Time: 24 hoursResult: Reduced VEGF mRNA expression.體內(nèi)研究 Tocilizumab (Anti-Human IL6R, Humanized Antibody) (intravenous inj

7、ection; 1.0 mg/100 L; twice a week, threeweeks) decreases the volume of each metastatic focus in tocilizumab-injected mouse brains2.Tocilizumab (Anti-Human IL6R, Humanized Antibody) (intraperitoneal injection;100 g/mL, every 48 hours; 20days)suppresses OSCC progression and suppresses Tumor Angiogene

8、sis3.Animal Model: A brain metastasis model of lung tumor cells by inoculating HARA-B cells in nudemice2Dosage: 1.0 mg/100 LAdministration: Intravenous injection; 1.0 mg/100 L; twice a week, three weeksResult: Led the volume of each metastatic focus significantly smaller.Animal Model: SCID mice with

9、 SAS cells3Dosage: 100 g/mLAdministration: Intraperitoneal injection;100 g/mL; every 48 hours; 20daysResult: Suppressed the growth of advanced tumors.REFERENCES1. Garbers C, et al. Inhibition of classic signaling is a novel function of soluble glycoprotein 130 (sgp130), which is controlled by the ra

10、tio of interleukin 6and soluble interleukin 6 receptor. J Biol Chem. 2011 Dec 16;286(50):42959-70.2. Mami Noda, et al. IL-6 Receptor Is a Possible Target against Growth of Metastasized Lung Tumor Cells in the Brain. Int J Mol Sci. 2013 Jan; 14(1):515526.3. Shinriki S, et al. Humanized anti-interleukin-6 receptor antibody suppresses tumor angiogenesis and in vivogrowth of human oral squamous cellcarcinoma. Clin Cancer Res. 2009 Sep 1;15(17):5426-34.McePdfHeightPage 2 of 3 www.MedChemECaution: Product has not been fully validated for m