重組小鼠血管內(nèi)皮生長(zhǎng)因子165作用機(jī)制 - Medchemexpress - MCE中國(guó)

1、Product Data SheetRecombinant Mouse VEGF165Cat. No.: HY-P7312Synonyms: rMuVEGF165; VEGF-A; VPFSpecies: MouseSource: E. coliAccession: Q00731Gene ID: 22339Molecular Weight: Approximately 38.8 kDaPROPERTIESAA SequenceM A P T T E G E Q K S H E V I K F MD V Y Q R S Y C R P I E T L V D I F Q E Y P D E I

2、E Y I F K P S C V P L M R C A G C C N D E A L E C V P T S E S N I T MQ ID K P R RM R I K P H Q S Q H I G E M S F L Q H S R C E C R P K K D R T K P E K H C E P C S E R R K H L F V Q D P Q T C K C S C K N T D S R C K A R Q L E L N E R T C R CBiological Activity The ED50 is 2.0 105 units/mg.Appearance

3、Lyophilized powder.Formulation Lyophilized after extensive dialysis against PBS, pH 7.4.Endotoxin Level 1 EU/g, determined by LAL method.Reconsititution Reconstitute the lyophilized recombinant Mouse VEGF165 (rMuVEGF165) to 0.1-1.0 mg/mL using sterile distilledwater or aqueous buffer containing 0.1%

4、 BSA.Storage & Stability Lyophilized recombinant Mouse VEGF165 (rMuVEGF165) is stored at -20C. After reconstitution, it is stable at 4C for1 week or -20C for longer. It is recommended to freeze aliquots at -20C or -80C for extended storage.Shipping Room temperature in continental US; may vary elsewh

5、ere.DESCRIPTIONBackground Vascular endothelial growth factor (VEGF or VEGFA) is a potent mediator of both angiogenesis and vasculogenesis inthe fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conservedcysteine residues and a cystine knot structure1.

6、VEGF165 appears to be the most abundant and potent isoform,followed by VEGF121 and VEGF18912. VEGF165 binds the semaphorin receptor, Neuropilin1 and promotesPage 1 of 2 www.MedChemEcomplex formation with VEGF R23.REFERENCES1. Robinson CJ, et al. The splice variants of vascular endothelial growth fac

7、tor (VEGF) and their receptors. J Cell Sci. 2001 Mar;114(Pt 5):853-65.2. Byrne AM, et al. Angiogenic and cell survival functions of vascular endothelial growth factor (VEGF). J Cell Mol Med. 2005 Oct-Dec;9(4):777-94.3. Qi Pan, et al. Neuropilin-1 Binds to VEGF121 and Regulates Endothelial Cell Migration and Sprouting. The Journal of Biological Chemistry 282, 24049-24056.McePdfHeightCaution: Product has not been fully validated for medical applications. For research use only.Tel: 40