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1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEGinsenoside Rh1Cat. No.: HY-N0604CAS No.: 63223-86-9Synonyms: Prosapogenin A2; Sanchinoside B2; Sanchinoside Rh1分式: CHO分量: 638.87作靶點: PPAR; TNF Receptor; Interleukin Related作通路: Cell Cycle/DNA Damage; Apoptosis;Immunology/Inflam
2、mation儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 100 mg/mL (156.53 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 1.5653 mL 7.8263 mL 15.6526 mL5 mM 0.3131 mL 1.5653 mL 3.1305 mL10 mM 0.1565 mL 0.7826 mL 1.5653 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度,選擇合
3、適的溶劑配制儲備液,并請注意儲備液的保存式和期限。體內(nèi)實驗請根據(jù)您的實驗動物和給藥式選擇適當?shù)娜芙獍福渲魄罢埾扰渲瞥吻宓膬湟?,再依次添加助溶?為保證實驗結果的可靠性,體內(nèi)實驗的作液,建議您現(xiàn)現(xiàn)配,當天使;澄清的儲備液可以根據(jù)儲存條件,適當保存;以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占):1. 請依序添加每種溶劑: 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (3.91 mM); Clear solution2. 請依序添加每種溶劑: 10% DMSO 90% (20% SBE-CD in sal
4、ine)Solubility: 2.5 mg/mL (3.91 mM); Clear solution1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE3. 請依序添加每種溶劑: 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (3.91 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Ginsenoside Rh1 (Prosapogenin A2; Sanchinoside B2; Sanchinoside Rh1)是從 Panax Ginseng 根部分離的。Gins
5、enoside Rh1 抑制 PPAR-,TNF-,IL-6 和 IL-1 的表達。IC50 & Target PPAR- IL-6 IL-1 TNF-體外研究 The effect of Ginsenoside Rh1 is examined on adipogenesis in 3T3-L1 cells. Ginsenoside Rh1 potentlyinhibits the adipogenesis, as assessed by Oil-red O staining and lipid contents in 3T3-L1 adipocytes.Ginsenoside Rh1, at
6、 concentrations of 50 M and 100 M, inhibit the adipogenesis by 50% and 63%,respectively.The expression levels of adipocytespecific genes such as PPAR-, C/EBP-, FAS, aFABP andsome genes are examined during early phase of differentiation such as Pref-1, C/EBP- and Glucocorticoidreceptor (GR). After th
7、e treatment with Ginsenoside Rh1 in 3T3-L1 cells, mRNA is extracted on 18 h and 24h for Pref-1, C/EBP- and GR and day 8 for PPAR-, C/EBP-, FAS, aFABP. Then, the expression profiles ofadipocyte-specific genes are investigated by RT-PCR. PPAR-, C/EBP-, FAS, and aFABP expressions aresignificantly incre
8、ased in DMI-stimulated differentiated adipocyte compared to those of non-stimulatedadipocyte cells. However, treatment with DMI in the presence of Ginsenoside Rh1 significantly suppressesthe expression levels of PPAR-, C/EBP-, FAS, and aFABP in a dose- dependent manner, whereas theexpression levels
9、of Pref-1, C/EBP- and GR are not affected 1.體內(nèi)研究 When high-fat diet (HFD) fed mice for 8 weeks, body and epididymal fat weight gains are significantlyincreased compared to those of low-fat diet (LFD)-fed mice. However, when Ginsenoside Rh1 is treated inHFD-fed mice, body and epididymal fat weight ga
10、ins are significantly decrease compared with those of theHFD-fed mice. TG, glucose, insulin, total cholesterol, and HDL levels in the blood are significantly increasedin HFD-fed mice group compared to LFD-fed mice group. Treatment with Ginsenoside Rh1 in HFD-fed micesignificantly lowers TG level alo
11、ne 1.PROTOCOLCell Assay 1 Mouse embryo fibroblasts 3T3-L1 cells are incubated in DMEM, containing 10% FBS and 1% AB, at 37Cand 5.6% CO2 atmosphere. To induce differentiation, two days after confluence, preadipocytes (designatedday 0) are cultured in the differentiation medium (DM), which is consiste
12、d of DMEM, 10% FBS, 1% AB, andDMI (0.28 unit/mL insulin, 0.5 mM Isobutylmethylxanthine and 1 M Dexamethasone) for 2 d in the presenceor absence of 50 M and 100 M of Ginsenoside Rh1, and switched to DM containing 10% FBS and 10 g/mL insulin and then changed to DMEM medium with 10% FBS for every 2 d 1
13、.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice 1Administration 1 Male C57BL/6J mice are separated into 3 groups, LFD, HFD and HFDRh1. Each group is consisted of ten2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemEmice. LFD group fed LFD fo
14、r 8 weeks. HFD group fed HFD for 8 weeks. HFDRh1 group fed HFD diet for 4weeks and then simultaneously treated with HFD and 20 mg/kg/d Ginsenoside Rh1, which is orallyadministrated. Weight and food intake of mice are measured daily. After finishing treatment for 4 weeks,blood and epididymal fats are collected for further analysis.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Gu W, et al. Ginsenoside Rh1 ameliorates high fat diet-induced obesity in mice by inhibiting adipocyte differentiation. Biol Pharm
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