Sulforhodamine-101-SR101-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESulforhodamine 101Cat. No.: HY-101878CAS No.: 60311-02-6Synonyms: SR101分式: CHNOS分量: 606.71作靶點(diǎn): Others作通路: Others儲(chǔ)存式: -20C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 75 mg/

2、mL (123.62 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 1.6482 mL 8.2412 mL 16.4823 mL5 mM 0.3296 mL 1.6482 mL 3.2965 mL10 mM 0.1648 mL 0.8241 mL 1.6482 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn) 請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前?qǐng)先配制澄清的儲(chǔ)備液，再依次添加助溶劑(為保

3、證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (4.12 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (4.12 mM); Clear solution1/2 Master of Small Molec

4、ules 您邊的抑制劑師www.MedChemEBIOLOGICAL ACTIVITY物活性 Sulforhodamine 101種紅熒光染料。體外研究 Sulforhodamine 101 does not label astrocytes in brainstem slices as strong and specific as in thehippocampus or cortex. To minimize excitatory side effects, the concentration of Sulforhodamine 101 has tobe kept as low as po

5、ssible or the labeling procedure can be performed after the actual experiment 1.體內(nèi)研究 In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 MSulforhodamine 101 or topical application of 100 M 1.PROTOCOLCell Assay 1 Acute brain slices are usually incubated in

6、 carbonated extracellular solution containing 0.5 to1 MSulforhodamine 101 for 20 to 30 min and 34 to 37C. Following this, excess dye is removed over a period of10 to 30 min using different protocols that were described earlier 1.MCE has not independently confirmed the accuracy of these methods. They

7、 are for reference only.Animal For in vivo imaging, Sulforhodamine 101 (SR101) is applied topically at concentrations of 250 nM to 300 MAdministration 1 or by bolus injection. Additionally, Sulforhodamine 101 injection over the tail vein (10 mg/mL) has beenreported to be successful 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Hlsmann S, et al. Limitations of Sulforhodamine 101 for Brain Imaging. Front Cell Neurosci. 2017 Feb 28;11:44.McePdfHeightCaution: Product has not been fully validated for medical applications.For