常用熒光染料激發(fā)和發(fā)射波長

1、常用熒光染料激發(fā)和發(fā)射波長常用熒光染料激發(fā)和發(fā)射波長常用熒光染料激發(fā)和發(fā)射波長適用標準文檔熒光素名稱激發(fā)波長發(fā)射波長備注(nm)(nm)1,5IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein(5-FAM)4925185-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine(5-TAMRA)5425685-F

2、AM(5-Carboxyfluorescein)4925185-HAT(HydroxyTryptamine)370-415520-5405-HydroxyTryptamine(HAT)370-415520-5405-ROX(carboxy-X-rhodamine)5786045675915-TAMRA(5-Carboxytetramethylrhodamine)5485525425686-Carboxyrhodamine6G5185436-CR6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-AminoactinomycinD(7-AAD)5

3、466477-Hydroxy-4-methylcoumarin360449,4559-Amino-6-chloro-2-methoxyacridine412,430471,474ABQ344445AcidFuchsin540630ACMA(9-Amino-6-chloro-2-412,430471,474methoxyacridine)AcridineOrange+DNA502526AcridineOrange+RNA460650AcridineOrange,bothDNA&RNA440-480520-650AcridineRed455-600560-680AcridineYellow4705

4、50Acriflavin436520AcriflavinFeulgenSITSA355-425460Aequorin(Photoprotein)466PhotoproteinAFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFPAlexaFluor350?346442342441AlexaFluor430?431540AlexaFluor488?495,492519,520AlexaFluor532?531,532553,554文案大全適用標準文檔AlexaFluor546?556,557572,573AlexaFl

5、uor568?577,578603AlexaFluor594?590,594617,618AlexaFluor633?632650AlexaFluor647?647666AlexaFluor660?668698AlexaFluor680?679702AlizarinComplexon530-560,580,624-580645AlizarinRed530-560580Allophycocyanin(APC)630,645655,660AMC,AMCA-S345445AMCA(Aminomethylcoumarin)345425347444AMCA-X353442Aminoactinomycin

6、D555655Aminocoumarin346442350445Aminomethylcoumarin(AMCA)345425347444AnilinBlue600Anthrocylstearate360-381446APC(Allophycocyanin)630,645655,660APC-Cy7625-650755APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505AstrazonBrilliantRed4G500585AstrazonOrangeR470540AstrazonRed6B520595AstrazonYell

7、ow7GLL450480Atabrine436490ATTO-TAG?CBQCA465560ATTO-TAG?FQ486591Auramine460550AurophosphineG450580Aurophosphine450-490515BAO9(Bisaminophenyloxadiazole)365395BCECF(highpH)492,503520,528BCECF(lowpH)482520BerberineSulphate430550BetaLactamase409447,520BFPblueshiftedGFP(Y66H)381,382,445,447,blueshiftedGFP

8、文案大全適用標準文檔BlueFluorescentProtein383448(Y66H)BlueFluorescentBFP/GFPFRETProteinBimane398490Bisbenzamide360461Bisbenzimide(Hoechst)360461bis-BTC=RatioDye,Zn2+455/405529/505RatioDye,Zn2+BlancophorFFG390470BlancophorSV370435BOBO?-1462481BOBO?-3570602Bodipy492/515490515Bodipy493/503533549Bodipy500/5105095

9、15Bodipy505/515502510Bodipy530/550528547Bodipy542/563543563Bodipy558/568558569Bodipy564/570564570Bodipy576/589579590Bodipy581/591584592Bodipy630/650-X625642Bodipy650/665-X647665Bodipy665/676605676BodipyFl504,505511,513BodipyFLATP505514BodipyFl-Ceramide505511BodipyR6GSE528547BodipyTMR542574BodipyTMR-

10、Xconjugate544573BodipyTMR-X,SE544570BodipyTR589617BodipyTRATP591620BodipyTR-XSE588616BO-PRO?-1462481BO-PRO?-3544570BrilliantSulphoflavinFF430520BTC-RatioDyeCa2+464/401533/529RatioDyeCa2+BTC-5N-atioDye,Zn2+459/417517/532RatioDye,Zn2+Calcein494517CalceinBlue373440文案大全適用標準文檔CalciumCrimson?588,589611,61

11、5CalciumGreen501,506531CalciumGreen-1Ca2+Dye506531Ca2+DyeCalciumGreen-2Ca2+506/503536Ca2+CalciumGreen-5NCa2+506532Ca2+CalciumGreen-C18Ca2+509530Ca2+CalciumOrange549575576CalcofluorWhite385,395,437,440,405445Carboxy-X-rhodamine(5-ROX)576601CascadeBlue?377420398423399CascadeYellow399550400552Catechola

12、mine410470CCF2(GeneBlazer)CFDA494520CFP-CyanFluorescentProtein430,433,474,475,CyanFluorescent436,(453)476,(501)ProteinCFP/YFPFRETChlorophyll480650ChromomycinA436-460470ChromomycinA445575CL-NERF(RatioDye,pH)504/514540RatioDye,pHCMFDA494520Ca2+Dye,CoelenterazineCa2+Dye,bioluminescence(429)465biolumine

13、scence,nativemoleculeCoelenterazinecp(Ca2+Dye,)(430)442Ca2+Dye,bioluminescenceCoelenterazinef(437)473Ca2+Dye,bioluminescenceCoelenterazinefcp452Ca2+Dye,bioluminescenceCoelenterazineh(437)464Ca2+Dye,bioluminescenceCoelenterazinehcp(433)444Ca2+Dye,bioluminescenceCoelenterazineip441Ca2+Dye,bioluminesce

14、nceCoelenterazinen(431)467Ca2+Dye,文案大全適用標準文檔CoelenterazineO460575bioluminescenceCoumarinPhalloidin387470C-phycocyanineCPMMethylcoumarin384469MethylcoumarinCTC400-450602CTCFormazanCy2?489506Cy3.18554568Cy3.5?581598Cy3?514566552570554Cy5.18649666Cy5.5?675695Cy5?649666670Cy7?710,743767,805CyanGFP433(45

15、3)475(501)cyclicAMPFluorosensor(FiCRhR)500517CellProliferationCyQuantCellProliferationAssay480520AssayDabcyl453Dansyl340578DansylAmine337517DansylCadaverine335518DansylChloride372518DansylDHPE336517Dansylfluoride356noneDAPI359461Dapoxyl403580Dapoxyl2374574Dapoxyl3373574DCFDA504529DCFH(Dichlorodihydr

16、ofluorescein505535Diacetate)DDAO463607DHR(Dihydorhodamine123)505534Di-4-ANEPPS496705Di-8-ANEPPS(non-ratio)488605498713DiA(4-Di-16-ASP)456591文案大全適用標準文檔DichlorodihydrofluoresceinDiacetate505535(DCFH)DiD-LipophilicTracer644665LipophilicTracerDiD(DiIC18(5)644665DIDS341415Dihydorhodamine123(DHR)505535DiI

17、(DiIC18(3)549,551565Dinitrophenol349DiO(DiOC18(3)484,487501,502DiR748780LipophilicTracerDiR(DiIC18(7)750779DM-NERF(highpH)497/510540RatioDye,pHDNP349Dopamine340490-520DsRed558583RedfluorescentproteinDTAF494520HemicyaneDY-630-NHS621660labelforproteinsandDNAHemicyaneDY-635-NHS634664labelforproteinsand

18、DNAEBFP383447EnhancedBlueFluorescentProteinECFP436474EnhancedCyanFluorescentProteinEGFP488,498507,516EnhancedGreenFluorescentProteinELF97345530Eosin524545Erythrosin529,532554,555ErythrosinITC529555EthidiumBromide510,523595,605Ethidiumhomodimer-1(EthD-1)528617Euchrysin430540EukoLightEuropium(III)chlo

19、rideEYFP513,520527,532EnhancedYellowFluorescentProteinFastBlue360440FDA494520Feulgen(Pararosaniline)570625文案大全適用標準文檔FIF(FormaldehydInducedFluorescence)405433FITC490,494520,525FITCAntibody493517FlazoOrange375-530612Fluo-3480520,527506,506Fluo-4494516Fluorescein(FITC)490,494520,525FluoresceinDiacetate

20、494520Fluoro-Emerald495524Fluoro-Gold(Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM1-43?479598FM4-46515640FuraRed?(highpH)572657FuraRed?/Fluo-3Fura-2,highcalcium335505ExcitationratiodyeFura-2,lowcalcium363512ExcitationratiodyeFura-2/BCECFGenacrylBrilliantRedB520590GenacrylBrilliantYellow1

21、0GF430485GenacrylPink3G470583GenacrylYellow5GF430475GeneBlazer(CCF2)GFP(S65T)498516GFPredshifted(rsGFP)498516GFPwildtype,non-UVexcitation(wtGFP)475509GFPwildtype,UVexcitation(wtGFP)395509GFPuv385508GloxalicAcid405460GranularBlue355425Haematoporphyrin530-560580Hoechst33258345487Hoechst33342347483Hoec

22、hst34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine(FluoroGold)361536Hydroxytryptamine400530Indo-1,highcalcium330401EmissionratiodyeIndo-1,lowcalcium346475Emissionratiodye文案大全適用標準文檔Indodicarbocyanine(DiD)644665Indotricarbocyanine(DiR)748780IntrawhiteCf360430JC-1514529JO-JO-15305

23、45JO-PRO-1532544LaserPro795812Laurodan355460LDS751(DNA)543712LDS751(RNA)590607LeucophorPAF370430LeucophorSF380465LeucophorWS395465LissamineRhodamine572,577591,592LissamineRhodamineB577592LIVE/DEADKitAnimalCells494517formoredetailsreferCalcein/Ethidiumhomodimer528617LOLO-1566580LO-PRO-1568581LuciferY

24、ellow425,428528,536,540LysoTrackerBlue373422LysoTrackerBlue-White466536LysoTrackerGreen504,534511,551LysoTrackerRed490516LysoTrackerYellow551576LysoSensorBlue374424LysoSensorGreen442505LysoSensorYellow/Blue384540MagGreen507531MagdalaRed(PhloxinB)524600Mag-FuraRed483/427659/631RatioDye,Mg2+Mag-Fura-2

25、369/329508RatioDyeCa2+369/330511/491RatioDyeMg2+Mag-Fura-5369/330505/500RatioDye,Ca2+369/332505/482RatioDye,Mg2+Mag-Indo-1349/328480/390RatioDye,Ca2+349/330480/417RatioDye,Mg2+MagnesiumGreen506,507531MagnesiumOrange550575MalachiteGreen628MarinaBlue362459文案大全適用標準文檔MaxilonBrilliantFlavin10GFF450495Max

26、ilonBrilliantFlavin8GFF460495Merocyanin555578Methoxycoumarin360410MitotrackerGreenFM490516MitotrackerOrange551576MitotrackerRed578599Mitramycin450470Monobromobimane398490Monobromobimane(mBBr-GSH)398500Monochlorobimane380461MPS(MethylGreenPyronineStilbene)364395NBD466539NBDAmine450530NileRed515-555,5

27、90,640559Nitrobenzoxadidole465510-650Noradrenaline340490-520NuclearFastRed289-530580NuclearYellow365495NylosanBrilliantIavinE8G460510OregonGreen503522OregonGreen488-X494517OregonGreen?503522OregonGreen?488490,493514,520OregonGreen?500497517OregonGreen?514506526PacificBlue405455Pararosaniline(Feulgen

28、)570625PBFI340/380420ExcitationratiodyePE-Cy5488670PE-Cy7488755,767PerCP488675PerCP-Cy5.5488710PE-TexasRedRed613488613PhloxinB(MagdalaRed)524600PhorwiteAR360430PhorwiteBKL370430PhorwiteRev380430PhorwiteRPA375430Phosphine3R465565PhotoResist365610文案大全適用標準文檔PhycoerythrinBPE546-565575PhycoerythrinRPE565

29、578PKH26(Sigma)551567PKH67496520ChromaPMIA341376PontochromeBlueBlack535-553605POPO-1433457POPO-3533574PO-PRO-1435455PO-PRO-3539567Primuline410550ProcionYellow470600PropidiumIodid(PI)(305),617536,538PyMPO412,415561,564,570Pyrene360387Pyronine410540PyronineB540-590560-650PyrozalBrilliantFlavin7GF36549

30、5QSY7560QuinacrineMustard440510Red613PE-TexasRed488613Resorufin571584,585RH414532716Rhod-2552576Rhodamine550573Rhodamine110496,497520Rhodamine123507529Rhodamine5GLD470565Rhodamine6G525555RhodamineB540625RhodamineB200523-557595RhodamineBextra550605RhodamineBB540580RhodamineBG540572RhodamineGreen50252

31、7RhodaminePhallicidine558,542575,565RhodaminePhalloidine542565RhodamineRed570590RhodamineWT530555RoseBengal525,540550-600文案大全適用標準文檔R-phycocyanineR-phycoerythrin(PE)565578rsGFP473509redshiftedGFP(S65T)S65A471504S65C479507S65L484510S65T488511SapphireGFP395511SBFI340/380420ExcitationratiodyeSerotonin36

32、5520-540SevronBrilliantRed2B520595SevronBrilliantRed4G500583SevronBrilliantRedB530590SevronOrange440530SevronYellowL430490sgBFP?387450QuantumssgBFP?(superglowBFP)387450SuperGlo?GFPsgGFP?474488AFPsQuantumssgGFP?(superglowGFP)474509SuperGlo?GFPAFPsSITS336436IonChannelsSITS(Primuline)395-425450SITS(Sti

33、lbeneIsothiosulphonicAcid)365460SNAFLcalcein506/535535/620RatioDye,pHSNAFL-1508/540543/623RatioDye,pHSNAFL-2514/543546/630RatioDye,pHSNARFcalcein552/574590/629RatioDye,pHSNARF1576/548635/587ExcitationandemissionratiodyeSodiumGreen506,507532Na+,K+SpectrumAqua433,/53480/55VysisSpectrumGreen497/30,538/

34、44,Vysis509/31524/56SpectrumOrange559/38,588/48Vysis560SpectrumRed587,612,587/35612/51SPQ(6-methoxy-N-(3-344443sulfopropyl)quinolinium)文案大全適用標準文檔Stilbene335440SulphorhodamineBcanC520595SulphorhodamineGExtra470570SYTO11508,510527,530DyeforDNA,RNASYTO12499,500522,519DyeforDNA,RNASYTO13488,491509,514Dy

35、eforDNA,RNASYTO14517,521549,547DyeforDNA,RNASYTO15516,518546,555DyeforDNA,RNASYTO16488,494518,525DyeforDNA,RNASYTO17621634DyeforDNASYTO18490,493507,527DyeforDNA,RNASYTO20512530DyeforDNASYTO21494517DyeforDNASYTO22515535DyeforDNASYTO23499520DyeforDNASYTO24490515DyeforDNASYTO25521556DyeforDNASYTO404204

36、41DyeforDNASYTO41430454DyeforDNASYTO42433460DyeforDNASYTO43436467DyeforDNASYTO44446471DyeforDNASYTO45452484DyeforDNASYTO59622645DyeforDNASYTO60652678DyeforDNASYTO61628645DyeforDNASYTO62652676DyeforDNASYTO63657673DyeforDNASYTO64599619DyeforDNASYTO80531545NucleicAcidStainSYTO81530544NucleicAcidStainSY

37、TO82541560NucleicAcidStainSYTO83543559NucleicAcidStainSYTO84567582NucleicAcidStainSYTO85567583NucleicAcidStainSYTOXBlue445470NucleicAcidStainSYTOXGreen504523NucleicAcidStainSYTOXOrange547570NucleicAcidStainTetracycline390-425525-560Tetramethylrhodamine(TRITC)555576TexasRed?595620TexasRed-X?conjugate

38、595615文案大全適用標準文檔Thiadicarbocyanine(DiSC3)651,653674,675ThiazineRedR596615ThiazoleOrange510530Thioflavin5430550ThioflavinS430550ThioflavinTCN350460Thiolyte370-385477-488ThiozoleOrange453480TinopolCBS(CalcofluorWhite)390430TMR550573TO-PRO-1515531TO-PRO-3644657TO-PRO-5747770TOTO-1514531,533TOTO-3642660TriColor(PE-Cy5)(488)650667TRITCTetramethylRodamineIsoThioCyanate550573TrueBlue365425TruRed490695Ultralite656678UranineB42052