genius水冷ndyag激光文獻(xiàn)選輯

1、信Author: AA WAvander AJvanGAvanderNd:YAG 激光信Author: AA WAvander AJvanGAvanderNd:YAG 激光非手術(shù)療法在老年患者中的應(yīng)用作者：向?qū)W應(yīng)用Nd:YAG 激光減輕牙周刮治術(shù)中疼痛的臨床觀作者段向Nd:YAG 水激光在廣泛型侵襲性非手術(shù)治療的作者向?qū)W的短期臨床療效觀作者葉 學(xué)Nd:YAGlaserclinicalassistedinclassIIfurcationAuthor:Ana Karina odeAndrade&IlriaSalomoFeist&CludioMendesPannutiSilvanaCai&Denis

2、eMariaZezell&ioDeLong-termeffectsofasingleapplicationofawater-cooledNd:YAGlaserinsupplementtoscalingandrootplaninginpatientswith periodontal inflammationAuthor:TalatQadri&FawadJaved&PavlinaPoddani&JanTunr&EvaluationofsurgicaltreatmentofperiodontalpocketswithRoyal Dental College,University 信致閱我，Thork

3、ildKarring, 是奧胡斯大。本次合作研究的合作伙伴包括了牙、Genius 激光公司經(jīng)過(guò)奧胡斯牙以及丹麥貿(mào)易洋行的努力確認(rèn)了經(jīng)過(guò)多次改水冷激光系統(tǒng)是信致閱我，ThorkildKarring, 是奧胡斯大。本次合作研究的合作伙伴包括了牙、Genius 激光公司經(jīng)過(guò)奧胡斯牙以及丹麥貿(mào)易洋行的努力確認(rèn)了經(jīng)過(guò)多次改水冷激光系統(tǒng)是牙周治療的合作設(shè)備，原因如率的切，同時(shí)保持水/氣噴霧專(zhuān)利的冷卻效果使得：激光可以長(zhǎng)時(shí)間作用于組織；可忽略不計(jì)的氣徹底避免軟水冷激光專(zhuān)為牙周治療設(shè)計(jì)，同時(shí)配合嚴(yán)謹(jǐn)?shù)呐嘤?xùn)教育體系Genius 水冷激光的優(yōu)點(diǎn)是：水/氣噴霧專(zhuān)利，保證獨(dú)一無(wú)二的優(yōu)化激光參數(shù)可以無(wú)無(wú)痛的切割為了獲得

4、最佳的切割效果，必須盡量縮短脈沖的持續(xù)時(shí)間（等同保證安全無(wú)痛和無(wú)血手術(shù)，需要對(duì)軟組織有一血，同時(shí)短到保持足夠的切割效果是組織處于持續(xù)冷卻環(huán)Genius 水冷激光經(jīng)過(guò)奧胡斯大牙 1、水冷N(xiāo)d-YAG 激光對(duì)牙周袋齦下潔2、水冷N(xiāo)d-YAG 激光對(duì)牙周袋手術(shù)潔最終：Thorkild Karring 教授認(rèn)為此兩項(xiàng)研究表明了激光治療獲得的臨床改善同常規(guī)方水/氣瀑布配合下的Genius 水冷激光水/氣瀑布配合下的 Genius 水冷激光是專(zhuān)利設(shè)備，水從光纖的同一管道中傳輸。這Genius 水冷激光采用水/氣瀑布的目的enus水冷激光不但有熱效應(yīng)，同時(shí)還有可控制的等離子消融再生修復(fù)術(shù)效應(yīng)。水/氣水/氣瀑

5、布消除了組織碳化、預(yù)防了熱傳導(dǎo)對(duì)周?chē)M織優(yōu)化參數(shù)、使用水/氣瀑布配合的激光，理所當(dāng)然是牙周治療的好方Genius 水冷激光就有這樣優(yōu)異表現(xiàn)：無(wú)痛、切割效率、沒(méi)有組織碳化；同時(shí)保持完ThorkildKarring 口腔及牙周丹麥奧胡斯牙SHORT COMMUNICATIONAA Kranendonk AJvanWinkelhoff GAvanderThe bactericidal effect of Nd:YAG Authors A.A.Kranendonk,G.A.vanderWeijden, Department of Periodontology, Academic Centre for

6、Dentistry Amsterdam, Amsterdam, The NetherlandsA.A.Kranendonk,G.A.vanderWeijden,Clinic for Periodontology, Utrecht,The W.A.vanderSHORT COMMUNICATIONAA Kranendonk AJvanWinkelhoff GAvanderThe bactericidal effect of Nd:YAG Authors A.A.Kranendonk,G.A.vanderWeijden, Department of Periodontology, Academic

7、 Centre for Dentistry Amsterdam, Amsterdam, The NetherlandsA.A.Kranendonk,G.A.vanderWeijden,Clinic for Periodontology, Utrecht,The W.A.vanderReijden,A.J.vanWinkelhoff, Department of Oral Microbiology, Academic Centre for Dentistry Amsterdam, Amsterdam, The Netherlands: e: To evaluate the in vitro ef

8、fectoftheNd:YAGlaser(Genius,MlsgaardCopenhagen,Denmark)onsixperiodontalMethods:sofsixdifferentperiodontalitans,gingivalis,Prevo ermedia,TannerellaFusobacteriumnucleatumandParvimonasmicra)preparedinsmallEppendorfftubes,andedto Nd:YAGlaserforfivedifferentperiodsoftime.Laserer6 Watt(onascaleof112W), 50

9、Hz,Pulseduration250ls.AfteruretotheCorrespondence FridusvanderDepartment of AcademicCentreforDentistryAmsterdam Louwesweg 11066EA Amsterdam The Netherlands.: +31-20-51 88 Fax: +31-20-51 88 : HYPERLINK mailto:ga.vd.weijdenacta.nl aliquots of the s were spread on blood platesforbacterialcounting. Resu

10、lts:After5sofure,therewasaotalcolonyformingforallsixselectedanisms.After15,30and45s,acterial cells could be :invitro,15sofNd:YAGlaserusewasfoundtoeffectivefortotalkillingofthesixtestedKey words: in vitro; laser; anisms; Accepted 22 September Most treatment modalities used in periodontal therapy aim

11、reduce the bacterial plaque on the root surface to levels ible with periodontal health. The effectiveness of scaling and root planing in the treatment of periodontal disease is TocitethisJDentHygiene8,2010;: 10.1111/j.1601-KranendonkAA,vanderReijdenWA,vansally accepted (1, 2). Some studies periodont

12、al treatment is better if particular can no longer be detected after therapy andt e ed t sites which breakdown J,vanderWeijdenGA.TheeffectofaGenius Nd:YAG2009 The Journal compilation 2009 Blackwell itive are at greater risk of Laser s ique t make it useful JDentHygiene8,2010;Kranendonk et al. Bacter

13、icidal effect of a Nd:YAG a variety of applications in dental care. It is capable of In vitro Small Eppendorff tubes were used in an in vitro experiment to study the microbiological effects of laser treatment on the killing of periodontal pathogens. Fresh clinical isolates of Aggregatibacter itans,

14、Porphyromonas gingivalis, Prevo ladia, Tannerella forsythia, Fusobacterium nucleatum and Pa and vapourizing plaque, and has the sulcular lining (46). aims atKranendonk et al. Bactericidal effect of a Nd:YAG a variety of applications in dental care. It is capable of In vitro Small Eppendorff tubes we

15、re used in an in vitro experiment to study the microbiological effects of laser treatment on the killing of periodontal pathogens. Fresh clinical isolates of Aggregatibacter itans, Porphyromonas gingivalis, Prevo ladia, Tannerella forsythia, Fusobacterium nucleatum and Pa and vapourizing plaque, and

16、 has the sulcular lining (46). aims at reducing or eliminating be clinically useful to reduber of authors have n. A cidal effect of ight is one of the eraction. The high tal of neodymium-tlaser energy (4, 711). Absorption of micra were retrieved from subgingiva patients with chronic periodwere les m

17、ost important factors in doped yttrium aluminium laser emitting light is well ab bin. The energy is tran absorbed in hydroxyapitto use in a period vascularized studi transmitted wiemedium, pectrum. This haemin (5 mg l)1) and menadione (1 mg l)1) and incubated for 14 days at 37 C in 80% N2, 10% CO2 a

18、nd 10% H2 (13). Identifi- cationwasbasedonGramstain,cellandcolonymorphology, air tolerance, production of catalase and on a number of dcal reactions. tryptic soy-itanswas isolated on ycin (TSBV) plates bated in air with 5% CO2 at 37 C for 3 days (14). Isolates identified onthe basis ofcharacteristic

19、 colony morphologyn t nto s the like inner structure) on TSBV, itive catalase reaction the characteristics of laser light, direct and the fibre tip is not required 3% hydrogen peroxide and a set of specific enzymes LaBalmelesGrottes,Monta u-Vercieu,by et al. dentists and Bacterial 72 h and were sub-

20、cultured on blood agar base s were prepared in phosphate-he Netherlands, the water cooled Nd:Yag laser is used as an adjunct to the -surgical y 105 colony forming units per ml. ul in an Eppendorff PCR-vial. saline of test volume was ment of periodontitis, as has been suggested by etal.starting the e

21、xperiment, 20 ll was extracted and spread on The e of this study was to investigate the culture plate for a baseline performed in triplicate for each Measurements dal effect of ius Nd:YAG laser (Genius anism ure time. Before use, the laser ivated on a of carbon r. Bacterial s were prepared to AS, Ml

22、sgaard Dental, Copenhagen, by ed to a Nd:YAG laser for four different periods of mining the time nsary to kill MaterialandA solid s e crystal Nd:YAG laser (Genius Periodontal A S) was used at the settings, as shown in Table 1. The Genius Nd:YAG laser with water and air coolant has an optical fibre t

23、ip, approxima y the size of riodontal probe (0.6 mm in Table 1. Nd:YAGlaser600112 112 112 10100 100800 400800 Pulse energyFig. 1. Laser tip (on the left) o the tube (on the right) which was followed by microbial JDentHygiene8,2010;Kranendonk et al. Bactericidal effect of a Nd:YAG Table 2. Presence (

24、+) or absence () of cultivable microbiologicalspeciesonthecultureplatesrelatedtotheried out through -surgical scaling and root planing, or by surgical procedures aimed at the elimination of pockets. Furthermore, antibiotics and antiseptics are used as an adjunctive to mechanical debridement to obtai

25、n elimination of certain bacterial species.differenturetimeoftheNd:YAGlaserin ure time 5+)+)+)+)+)+)More recently, it has been Kranendonk et al. Bactericidal effect of a Nd:YAG Table 2. Presence (+) or absence () of cultivable microbiologicalspeciesonthecultureplatesrelatedtotheried out through -sur

26、gical scaling and root planing, or by surgical procedures aimed at the elimination of pockets. Furthermore, antibiotics and antiseptics are used as an adjunctive to mechanical debridement to obtain elimination of certain bacterial species.differenturetimeoftheNd:YAGlaserin ure time 5+)+)+)+)+)+)More

27、 recently, it has been t er suchastheNd:YAGlaser,maybeusefulfordestroyingpresumably by a thermal effect (9, 17, 18). The erNd:YAGlaseronaofactionofachia coli was studied. In a thermocouple equipped cuvette, a temperature rise up to 50 C was observed after the use of Aa,Aggregatibacter gingivalis; Pi

28、, itans;Pg,Porphyromonas ermedia; Tf, Tannerella forsythia;Pm,Parvimonasmicra;Fn,Fusobacteriumer output of 100 W for 23 s. This n 90%loss ofviability ofE.coli.Asa comparison,a 5, 15, 30 and 45 s. The tip of the laser fibre was ofE.coliwasheatedupto50 Cinawaterhisnated lcohol before use. The laser ti

29、p was placed therewas only a minimal loss of viability of E.coli.Itwascon- tthermalresponsebecauseofjustheatingthethe Eppendorff tube and small horizontal s made in the direction of the bottom during the four fluid is not a sufficient explanation for the killing ure times. Laser settings by the manu

30、facturer (on a scale er 6 W 112 W), E. coli. Therefore, othsesmaybeinvolvedduringexpo- sure to the Nd:YAG laser (18). However, using a 50 Hz, Pulse duration 250 ls. The water and air coolant, ical for the Genius laser, was not activated during this inviroinwhichagarplates(inoculated with were ed to

31、laser light, temperatures close to 100 C cedure to avoid dilution of the dissolved anisms. measured immedia y after radiation. This t compensate for the lack of water and air coolant, tubes were kept on ice during the experiment. After expo- sure to the laser, the solution was extracted from the tub

32、es and microbial plating was performed (Fig. 1). Solutions were plated on blood agar plates to test for survival of the specific periodontal pathogens. For negative controls, the same proce- dure was performed without activating the laser (0 s).antimicrobial effect within this particular is caused b

33、y a photo-thermal effect n by photo-effectsof the laser light itself a ggested by Gro nquist etal. (20). Meral et al. (19) evaluated the minimal energy levels ne sarytohaveabactericidaleffectonabacterialsuspen variousoralanisms.Insteadofsubmergingthe, they observed a distance of 10 mm. Within labora

34、tory setting, they t the addition of a (e.g.sheepblood)enhancedtheabsorptionofthelaserThe efficacy of the Nd:YAG lasers has also been for the photo-thermal disinfection of root s by Bergmans et al. (21). Although a different indication, Table 2 shows the presence or absence of bacterial growth speci

35、fic microbiological species on the culture plates in invir s used bare resemblance to the present gation. Laser irradiation was performed in wet root stto ure time of the Nd:YAG laser. As a control bacterial of hadbeeninoculatedthe baseline as well as ure to -activated faecalis(a relatively heat-res

36、istant bacterium). The laser appli- cation was found to be safe and to have a potential disinfection capacity (99.7% kill). In a comparable study by Wang et al. (21), the Er,Cr:YSGG laser was compared with the Nd:YAG laser. Both laser systems had a significant bactericidal effect on infected root c

37、s, but the Nd:YAG was more effective resulting in 98% kill. In a more basic in viroendodontic m , laser radiation was delivered to 12 ll E.faecalislaser (0 s) showed confluent growth on all culture plates of test bacteria (Fig. 2a and b). After 5 s of ure, a otal colony forming units of all test spe

38、cies was (Fig. 2c). After ure of 15 s, no colony forming units observed for any of the six selected microbiological Consequently at 30 and 45 s of ure, for all the six odontalpathogens,thecultureplatesweresterile(Fig.cultures in small capillary tubes. For t received total energy of 60 J, no acteria

39、were detected ing laser treatment (Rooney et al. The most frequently used method to reduce the numbers pu iveperiodontalpathogensfromtheperiodontalpocketmechanical debridement of the root . This can be JDentHygiene8,2010;Kranendonk et al. Bactericidal effect of a Nd:YAG Fig. 2. (a) Total colony form

40、ing units of Prevo laermediaatbaseline;(b)0sof ure; (c) 5 s of exure; (d) 15 s ofhe present experiment, energy settings were used at 2 Badersten A, Nilveus R, Egelberg J. Effect of -surgical peri- odontal therapy, II. Severely advanced periodontitis. J Clin Period- ontol 1984; 11: 6376.level as a cl

41、ear dose response n percentage of heliterature (7 he effective- ness teria killed andKranendonk et al. Bactericidal effect of a Nd:YAG Fig. 2. (a) Total colony forming units of Prevo laermediaatbaseline;(b)0sof ure; (c) 5 s of exure; (d) 15 s ofhe present experiment, energy settings were used at 2 B

42、adersten A, Nilveus R, Egelberg J. Effect of -surgical peri- odontal therapy, II. Severely advanced periodontitis. J Clin Period- ontol 1984; 11: 6376.level as a clear dose response n percentage of heliterature (7 he effective- ness teria killed and energy density used is 3 Mombelli A, id B, Rutar A

43、, Lang N. Persistence patterns 9). in contact time might Porphyromonas gingivalis, ens,anditans after mechanical therapy of eliminating periodontal pathogens as the degree of disease.JPeriodontol2000;71:and thereby sible elimination of peri- odontal 4 Cobb CM, McCawley TK, Killoy WJ. A preliminary s

44、tudy on takes he Eppendorff tubes. This elimination of effects of the Nd:YAG laser on root and subgingival flora in vivo. JPeriodontol1992; 63: 5 Ando Y, Aoki A, Watanabe H, Ishikawa I. Bactericidal effect is proportional to the amount of energy absorbed by the Energy is a product time); therefore,

45、it er and duration of si- ble to alter either the ure erbium YAG laser on periodontal bacteria. 19:6 Morlock BJ, Pippin DJ, Cobb CM, Killoy W, effect of Nd:YAG laser exure on root surfarg Med the depth er or duration of by Rapley JW. The when used as the thereby increas- the adjunct to root planning

46、: an in vitro study. J Periodontol 1992; , the experiment revealed the usefulness the Nd:YAG laser in a7 Dobson J, Wilson M. Sensitization of oral bacteria in bio function of ure killing by light from a er laser. Arch Oral Biol 1992; Iwase T, Saito T, Nara Y, Morioka T. Inhibitory effect of HeNe las

47、er on dental plaque de ition in hamsters. J Periodontal Res 1989; 24: 282283.Wilson M, Dobson J, Sarkar S. Sensitization of periodontopathogen- ic bacteria to killing by light from a low- er laser. Oral Microbiol Immunol 1993; 8: 182187.Wilson M. Bactericidal effect of laser light and its potential

48、use in the treatment of plaque-related diseases. Dent J 1994; 44: 181 As contemporary treatment aims at a reduction of supra and subgingival bacterial mass by and planing the root , the Nd:YAG laser might be usefuladjuncttothistreatmentineliminating11 Ben Hatit Y, Blum R, Severin C, , Jabro MH. his

49、in vitro effects of a pulsed Nd:YAG laser on subgingival bacterial flora and on cementum: an in vivo study. J Clin Laser Med Surg 1996; 14: 12 Rooney J, Midda M, Leeming J. A laboratory investigation of the bactericidal effect of a NdYAG laser. BrDentJ1994; 176: 61 , 15 s of Nd:YAG laser use was fou

50、nd be 13 Slots J. Selective medium for isolation of -1 Axelsson P, Lindhe itans.Jicrobiol1982;4:The significance of ma enance care in treatment of periodontal disease. J Clin Periodontol 1981; 8: 281 JDentHygiene8,2010;Kranendonk et al. Bactericidal effect of a Nd:YAG 14 Van Steenbergen MTJ, Van J,

51、Van der Mispel L, 18Kranendonk et al. Bactericidal effect of a Nd:YAG 14 Van Steenbergen MTJ, Van J, Van der Mispel L, 18 Bergmans L, Moisiadis P, Teughels W, Van meerbeek B, Quirynen M, Lambrechts P. Bactericidal effect of Nd:YAG laser irradiatioder Velden U, Abbas F, De Graaff J. Comparison of two

52、 mediafor24:itans. Jicrobiol someendodonticpathogensexEndodJ2006;39:19 Meral G, Tasar F, Kocago z S, Sener C. Factors affecting the Ward GD, Watson IA, Stewart-Tull DE et al. Bactericidal action of er Nd:YAG laser light on Escherichiacoliin saline suspen-.JAppl Microbiol 2000; 89: Lioubavina N, Jens

53、en J, Karring T. Evaluation of periodontal bacterial effects of Nd:YAG laser in vivo. rg Med 2003; 20 Gro nquist A, Wistro m J, Axner O, Monsen TJ. Bactericidal effect of pulsed 1,064 nm Nd:YAG laser light on Staphylococcus dermis is of photo-thermal origin. Laser Surg Med 2000; 27: 336340.ets with

54、Nd:YAG laser. J DentRes 1997; 77: (Spe no. 19258.17 Raffetto N. Lasers for initial periodontal therapy. Dent Clin North Am 2004; 48: 923936.l e) b-21 , Zhang C-f, Yin X-z. Evaluation of the of Er,Cr:YSGG, and Nd:YAG lasers in infected root s. J Endod 2007; 33: JDentHygiene8,2010;2014272the bodys nut

55、itionalake.Theelderlyusuallyhavealotof nity ability decreased. Nowadays，the periodontal NdYAG激光非手術(shù)療法在老chbgingivalscalingandrootplanning牙surgicaltreatment，drugtreatment，alwayshavesomecies，when used in elderly periodontitis patients. Considering theelderlytoleranceabilityofcomplextreatment，we向?qū)W熔，晏 鶯，楊

56、 梅-surgicalperiodontaltreatment.AtpresenttheYAG laser has been widely used in periodontal therapy. articlewillreportoncharacteristicsof2014272the bodys nutitionalake.Theelderlyusuallyhavealotof nity ability decreased. Nowadays，the periodontal NdYAG激光非手術(shù)療法在老chbgingivalscalingandrootplanning牙surgicalt

57、reatment，drugtreatment，alwayshavesomecies，when used in elderly periodontitis patients. Considering theelderlytoleranceabilityofcomplextreatment，we向?qū)W熔，晏 鶯，楊 梅-surgicalperiodontaltreatment.AtpresenttheYAG laser has been widely used in periodontal therapy. articlewillreportoncharacteristicsoftheelderly

58、-surgicalperiodontaltreatmenttechnologies，and士advantages of NdYAG he periodontalastoprovide theoreticalbasisforNdYAG lasertreatmentperiodontitisinelderlyment；NdYAG 學(xué)余篇近五年來(lái)負(fù)責(zé)參與各級(jí)科研項(xiàng)目十余項(xiàng)10 年很大差距。20世紀(jì)末，牙周病與全身健康和疾病聯(lián)系起來(lái)。慢性是人類(lèi)疾病中普遍存在 治療技術(shù)、NdYAG 激光在牙周治療中的優(yōu)越性等方面老年非手術(shù)治療；NdYAG 激The application prospect -surgi

59、cal treatmentn NdYAGlaserfor theelderly 根據(jù)病情發(fā)展調(diào)整方案1。選擇一種既能ated Hospital of Stomatology，Chongqing ety，how to improve life equality of the elderly is a problem us.Chronicperiodontitisissoheelderly.Itonlyaffectschewing，butalsoaffectsthedigestion，espe 1 1.牙電子Journal ofPractical Feb.2014Vol.7No.效和牙如2.的第六

60、并發(fā)癥2Humphrey等 發(fā)現(xiàn)，牙周病是冠心病的獨(dú)，2.藥物治藥物治療只能作為基礎(chǔ)治療等4口腔菌群不良口致1.Journal ofPractical Feb.2014Vol.7No.效和牙如2.的第六并發(fā)癥2Humphrey等 發(fā)現(xiàn)，牙周病是冠心病的獨(dú)，2.藥物治藥物治療只能作為基礎(chǔ)治療等4口腔菌群不良口致1.牙周特老年患者病情一般較重部一般都是遵循循證醫(yī)學(xué)原則有針對(duì)性用藥，合理和家的庭2.合治合治療是指通過(guò)調(diào)系。研究在消除分布均牙合關(guān)系協(xié)牙齒和牙周組織得到均勻的功能性外力刺激有利牙周組織2. 4 松牙固定術(shù)制此時(shí)動(dòng)度較大的牙齒已很難恢復(fù)但是不處2 牙20142723. 2 NdYAG 激光