免疫熒光分型指南共41張課件

1、免疫熒光分型指南丁桃加拿大公共衛(wèi)生署國家HV免疫實(shí)驗(yàn)室昆明20193OUREI免疫熒光分型指南概要樣品處理單克隆抗體組滴定補(bǔ)償熒色物絕對(duì)數(shù)校準(zhǔn)熒光球通用分析模板OUREI概要樣品處理溶解免洗的方法單克隆50-100全血溶解液抗體孵化10/15分鐘孵化10/15分鐘流式細(xì)胞儀分析OUREI樣品處理建議加入2%的多聚甲醛固定絕對(duì)記數(shù)時(shí)熒光球和血液移液的準(zhǔn)確性加過熒光球的樣品,必須在當(dāng)天上機(jī)分析在上機(jī)采集數(shù)據(jù)時(shí),應(yīng)在達(dá)到至少1微升血樣之后才能停止;即如果熒光球的濃度為每微升1000,應(yīng)收集到至少1000個(gè)熒光球才能停止采集OUREI建議一抗體組合OUREI一抗體組合不同的抗體組合雙色:Facscou

2、nt and Guava: CD3/CD4PLG: CD45/4三色:BD and BC: CD4/3/45. CD4/8/3四色:BD and BC: CD4/8/3/45OUREI不同的抗體組合應(yīng)使用哪種抗體組合?CD4/CDS/CD3CD16/CD19/CD3CD45/CD4CD45/CDSCD45/CD3/CD4CD45/CD3/CD8CD4應(yīng)使用哪種抗體組合?NIH AND CDC指南Cytometry IClinical CytometryOriginal ArticlesUse of CD45 Gating in Three and Four-Color FlowCytometr

3、ic Immunophenotyping: Guideline From TheNational Institute of Allergy and Infectious Diseases,Division of aidsCarol T. Schnizlein-Bick, Francis F. Mandy, Maurice R.G. OGorman, Helene Paxton.Janet K-A. Nicholson, Lance E Hultin, Rebecca S GelmanCynthia L wilkening, and Daniella LivnarVoL. 52/RR-2Reco

4、mmendations and ReportsGuidelines for Performing Single-Platform Absolute CD4* T-CellDeterminations with CD45 Gating for Persons Infectedwith Human Immunodeficiency virusFrancis F. Mandy, PhDJa stk a nelson mOUREINIH AND CDC指南cD45的使用CD45可標(biāo)記所有的白細(xì)胞CD45對(duì)淋巴細(xì)胞的表達(dá)大大高于單核細(xì)胞和巨噬細(xì)胞,故用來識(shí)別淋巴細(xì)胞OUREIcD45的使用cD45異相門

5、控:一個(gè)穩(wěn)固的方法樣品年齡cD45/ss異相門FS/SS均相門天CD4 FIT(4天Schnizlein-Bick et al. 2019OUREIcD45異相門控:一個(gè)穩(wěn)固的方法免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張課件免疫熒光分型指南共41張