DB45-T 2206.2-2020甘蔗品質(zhì)的分析方法 第2部分：還原糖分的測(cè)定（蘭-艾農(nóng)恒容法）-（高清可復(fù)制）

ICS65.020CCSB34

DB45廣西壯族自治區(qū)地方標(biāo)準(zhǔn)DB45/T2206.2—20202（）Analysismethodofsugarcanequalitypart2:determinationofAnalysismethodofsugarcanequalitypart2:determinationofreducedsugar（Lane-Eynonmethod）2020111320201220廣西壯族自治區(qū)市場(chǎng)監(jiān)督管理局發(fā)布DB45/T2206.2DB45/T2206.2—2020DB45/T2206.2—2020DB45/T2206.2—2020前??言本文件按照GB/T1.1—2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分：標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)定起草。本文件為DB45/T2206《甘蔗品質(zhì)的分析方法》的第2部分。本文件由廣西壯族自治區(qū)糖業(yè)發(fā)展辦公室提出并宣貫。本文件由廣西糖業(yè)標(biāo)準(zhǔn)化技術(shù)委員會(huì)歸口。（南寧II引??言XXXX《甘蔗品質(zhì)的分析方法》分為如下部分：12（蘭—艾農(nóng)恒容法——3——第4部分：……。效益。II效益。II甘蔗品質(zhì)的分析方法第2部分：還原糖分的測(cè)定（蘭—艾農(nóng)恒容法）范圍本文件規(guī)定了甘蔗汁中還原糖分的測(cè)定方法（蘭—艾農(nóng)恒容法）。本文件適用于甘蔗汁中還原糖分的測(cè)定。規(guī)范性引用文件（包括所有的修改單適用于本文件。GB/T6682分析實(shí)驗(yàn)室用水規(guī)格和實(shí)驗(yàn)方法GB/T10499糖料甘蔗試驗(yàn)方法QB/T2343.2赤砂糖試驗(yàn)方法3術(shù)語和定義3術(shù)語和定義本文件沒有需要界定的術(shù)語和定義。4原理75mL下滴定標(biāo)定過的堿性酒石酸銅溶液(已用還原糖標(biāo)準(zhǔn)溶液標(biāo)定)，根據(jù)樣品液消耗體積計(jì)算還原糖含量。5試劑GB/T6682蔗糖標(biāo)準(zhǔn)品。鹽酸：1.19g/mL。氫氧化鈉溶液：1mol/L。轉(zhuǎn)化糖標(biāo)準(zhǔn)溶液(1023.750120mL250mL9mL20～258d，然后用水稀釋至刻度，搖勻。吸取該溶液100mL1g1000mLpH3.0，pH2g（3～4）。1轉(zhuǎn)化糖標(biāo)準(zhǔn)溶液(2.5g/L（5.5）50mL200mL50.5mol/LQB/T2343.2乙二胺四乙酸二鈉溶液(40g/L40g250mL1000mL草酸鉀溶液(50g/L50g250mL1000mL四甲基藍(lán)溶液(10g/L1.0g100mL100mL儀器設(shè)備小型壓榨機(jī)。0.1g。容量瓶：100mL、250mL、1000mL。滴定管：50mL0.1mL。錐形瓶：300mL。燒杯：100mL、250mL。量筒：50mL、100mL。秒表。電爐。6.11濾紙：秒表。電爐。6.11濾紙：135g/m2。6.12濾網(wǎng)：100μm（100）。7測(cè)定步驟樣品制備將經(jīng)過小型壓榨機(jī)壓榨的蔗汁混勻后過100目100mL蔗汁于250mL容量瓶中，用量筒加入40mL草酸鉀溶液（5.9）mL乙二胺四乙酸二鈉溶液（5.8），混勻后加水至刻度，搖勻待用。注：證滴定終點(diǎn)總體積能恒容到75mL。錘度、蔗糖分按GB/T10499的規(guī)定執(zhí)行。還原糖分用兩只移液器分別取費(fèi)林氏乙液、甲液各10mL于300mL錐形瓶中，混勻。加入15mL水，再從滴定管中加入7.1中配制好待用的樣液25mL于錐形瓶內(nèi)，搖勻，放在鋪有石棉網(wǎng)的電爐上加熱，煮沸2min（用秒表控制），加入四甲基藍(lán)指示液（5.10）3滴，在保持沸騰的情況下繼續(xù)滴加樣液至藍(lán)色消失為2止，記錄滴定耗用樣液體積，滴定至終點(diǎn)時(shí)間控制在1min內(nèi)，使整個(gè)沸騰和滴定加操作總時(shí)間控制在3min以內(nèi)。7.3.2復(fù)檢按7.3.1的方法分別取乙液、甲液各10mL于300mL錐形瓶中，加入按預(yù)檢耗用滴定樣液體積計(jì)算的加水量（總體積75mL減去預(yù)檢耗用配制樣液體積再減去甲液、乙液體積20mL），再加入比預(yù)檢耗用配制樣液量少1mL～2mL的樣液于錐形瓶內(nèi)，搖勻，滴定方法與預(yù)檢相同，煮沸2min，滴定至終點(diǎn)時(shí)間控制在1min內(nèi)，記錄滴定耗用樣液的體積。計(jì)算及結(jié)果表示100mL按式(1)計(jì)算：

mT×100×ρ250

.............................................................................(1)式中：mL（g）；T——吸取配制樣液的體積，單位為毫升（mL）；ρ——蔗汁視密度（20℃），用蔗汁錘度查表A.1得到。8.2消耗的配制樣液含蔗糖量8.2消耗的配制樣液含蔗糖量消耗的配制樣液中含蔗糖質(zhì)量G，以g表示，按式(2)計(jì)算：Gm×V×S10000..............................................................................(2)式中：mL配制樣液含樣品質(zhì)量，單位為克（g）；滴定消耗配制樣液體積，單位為毫升（mL）；樣品蔗糖分，％。8.3還原糖分蔗汁還原糖分R，以質(zhì)量分?jǐn)?shù)(％)表示，按式(3)計(jì)算：R1000×f×Km×V式中：f——校正系數(shù)，由G值查表B.1得到；K——費(fèi)林氏溶液度校正系數(shù)；mL配制樣液含樣品質(zhì)量，單位為克（g）；V——滴定消耗配制樣液體積，單位為毫升（mL）；3取平行測(cè)定值的算術(shù)均值為測(cè)定結(jié)果，保留小數(shù)點(diǎn)后兩位。精密度在重復(fù)條件下獲得的兩次獨(dú)立測(cè)試結(jié)果的絕對(duì)差值不大于這兩個(gè)測(cè)定值的算術(shù)平均值的2％。44附錄A（資料性附錄）蔗汁錘度-視密度對(duì)照表蔗汁錘度-視密度對(duì)照表見表A.1。表A.1錘度-視密度對(duì)照表錘度(°Bx)視密度(20℃)錘度(°Bx)視密度(20℃)錘度(°Bx)視密度(20℃)錘度(°Bx)視密度(20℃)錘度(°Bx)視密度(20℃)0.00.997170.10.997560.20.997950.30.998340.40.998720.50.999110.60.999500.70.999890.81.000280.91.000671.01.001061.11.001451.21.001841.31.002231.41.002611.51.003001.61.003391.71.003781.81.004171.91.004562.01.004952.11.005342.21.005742.31.006132.41.006522.51.006912.61.007302.71.007692.81.008092.91.008483.01.008873.11.009273.21.009663.31.010063.41.010453.51.010843.61.011243.71.011633.81.012033.91.012434.01.012824.11.013224.21.013614.31.014014.41.014414.51.014804.61.015204.71.015604.81.016004.91.016405.01.016805.11.017195.21.017595.31.017995.41.018395.51.018795.61.019195.71.019555.81.019995.91.020406.01.020806.11.021206.21.021606.31.022006.41.022416.51.022816.61.023216.71.023626.81.024026.91.024427.01.024837.11.025237.21.025647.31.026047.41.026457.51.026857.61.027267.71.027667.81.028077.91.028488.01.028888.11.029298.21.029708.31.030118.41.030528.51.030938.61.031338.71.031748.81.032158.91.032569.01.032979.11.033389.21.033799.31.034209.41.034619.51.035039.61.035449.71.035859.81.036269.91.0366710.01.0370910.11.0375010.21.0379110.31.0383310.41.0387410.51.0391610.61.0395710.71.0399910.81.0404010.91.0408211.01.0412311.11.0416511.21.0420711.31.0424811.41.0429011.51.0433211.61.0437311.71.0441511.81.0445711.91.0449912.01.0454112.11.0458312.21.0462512.31.0466712.41.0470912.51.0475012.61.0479312.71.0483512.81.0487712.91.0491913.01.0496113.11.0500313.21.0504613.31.0508813.41.0513013.51.0517213.61.0521513.71.0525713.81.0530013.91.0534214.01.0538514.11.0542714.21.0547014.31.0551214.41.0555514.51.0559814.61.0564014.71.0568314.81.0572614.91.0576855表A.1錘度-視密度對(duì)照表（續(xù)）錘度(°Bx)視密度(20℃)錘度(°Bx)視密度(20℃)錘度(°Bx)視密度(20℃)錘度(°Bx)視密度(20℃)錘度(°Bx)視密度(20℃)15.01.0581115.11.0585415.21.0589715.31.0594015.41.0598315.51.0602615.61.0606915.71.0611215.81.0615515.91.0619816.01.0624116.11.0628416.21.0632716.31.0637016.41.0641416.51.0645716.61.0650016.71.0654416.81.0658716.91.0663017.01.0667117.11.0671717.21.0676117.31.0680417.41.0684817.51.0689117.61.0693517.71.0697817.81.0702217.91.0706618.01.0711018.11.0715318.21.0719718.31.0724118.41.0728518.51.0732918.61.0737318.71.0741718.81.0746118.91.0750519.01.0754919.11.0759319.21.0763719.31.0768119.41.0772519.51.0776919.61.0781419.71.0785819.81.0790219.91.0794720.01.0799120.11.0803520.21.0808020.31.0812420.41.0816920.51.0821320.61.0825820.71.0830220.81.0834720.91.0839221.01.0843621.11.0848121.21.0852621.31.0857121.41.0861621.51.0866021.61.0870521.71.0875021.81.0879521.91.0884022.01.0888522.11.0893022.21.0897522.31.0902022.41.0906622.51.0911122.61.0915622.71.0920122.81.0924722.91.0929223.01.0933723.11.0938323.21.0942823.31.0947323.41.0951923.51.0956423.61.0961023.71.0965623.81.0970123.91.0974724.01.0979224.11.0983824.21.0988424.31.0993024.41.0997624.51.1002124.61.1006724.71.1011324.81.1015924.91.1020525.01.1025125.11.1029725.21.1034325.31.1038925.41.1043525.51.1048225.61.1052825.71.1057425.81.1062025.91.1066726.01.1071326.11.1075926.21.1075926.31.1080626.41.1089926.51.1094526.61.1099226.71.1103826.81.1108526.91.1113127.01.1117827.11.1122527.21.1127227.31.1131827.41.1146527.51.1141227.61.1145927.71.1150627.81.1155327.91.1160028.01.1164728.11.1169428.21.1174128.31.1178828.41.1183528.51.1188228.61.1192928.71.1197728.81.1202428.91.1207129.01.1211929.11.1216629.21.1221429.31.1226129.41.1230829.51.1236529.61.1240429.71.1245129.81.1249929.91.1254630.01.1259430.11.1264230.21.12