在食物診斷的趨向和需要

TrendsandNeedsinFoodDiagnosticsLarissaKahr-BIT,AustriaMalinBr?nnback-TEKES,FinlandGabrielavonBlankenfeld-Enkvist-TEKES,FinlandAimoftheStudyInvestigatetechnologicaltrendsandneedsinFoodDiagnosticsUnderstandthefactorsthatshapeandinfluencethedevelopmentofthisfield.BureauforInternationalResearchandTechnologyCooperation-AustriaFocusBackgroundonfoodsafetymanagementtoolsDescriptionofthemethodscurrentlyusedandnewmethodsdevelopedThemarketforfooddiagnostics

BureauforInternationalResearchandTechnologyCooperation-AustriaDefinitionsFoodDiagnosticsappliesmodernmethodsdevelopedinhumaninvitrodiagnostictofoodRapidMethodscomprisesdifferenttypesoftestsMiniaturisedbiochemicalkitsAntibody-basedtestsNucleicacidhybridisation-basedtestsManual,semi-automatedorfullyautomated

BureauforInternationalResearchandTechnologyCooperation-AustriaDetectionBacteria,viruses,parasites,chemicals,biotoxins,heavymetalsandprionsInallstepsofthefoodchain-fromrawmaterialstoendproducts

BureauforInternationalResearchandTechnologyCooperation-AustriaFoodastestmatrixExtremelyvariedinitschemicalcompositionContainsmanydifferentingredientsMightcontainanintrinsicmicrobialfloraBureauforInternationalResearchandTechnologyCooperation-AustriaFrameFoodSafetyProcesscontrolismoreefficientthanproductcontrolThesafetyofaproductwithlowdefectratescannotbeguaranteedthroughsamplingandtestingThefocusischangingfromend-producttestingtoHACCPThishastobeconsideredwhendevelopingnewmethodswithintheconceptualframeoffoodsafetyBureauforInternationalResearchandTechnologyCooperation-AustriaMicrobiologicalmethodscanbedividedinto“traditional”or“conventional”methodsandrapidmethods

BureauforInternationalResearchandTechnologyCooperation-AustriaConventionalMethodsConventionalmethodsrelyonculturingmicroorgansimsontoagarplatesLaboriousandtime-consuming84%ofalltestsarebasedoncountingviablecellsRequiredbynationalandinternationalregulatoryagenciesforofficialcontrolBureauforInternationalResearchandTechnologyCooperation-AustriaRapidMethodsAdvantagesinanalysistimePossibilitytoeliminatelabourintensivestepsPotentialforautomationHowever,lengthyincubationproceduresarestillnecessaryFirstone-shiftmethodsunderdevelopmentELISAandPCRaredominatingLackofacommonvalidationprocedureMostlynotacceptedforofficialcontrolpurposesBureauforInternationalResearchandTechnologyCooperation-AustriaMethodsAdvancesinViableCellCountMethodsMiniaturisationandDiagnosticKits,BiochemicalIdentificationTechniquesAntibody-basedmethodsImmunomagneticSeparationNucleicacid-basedassaysBiosensorsMicroarraysFlowCytometryBacteriophage-BasedTechniquesATPbioluminescenceAdenylatekinasebioluminescenceRiboprintingandPulse-FieldGelElectrophoresisBureauforInternationalResearchandTechnologyCooperation-AustriaViablecellcountingWillremainimportantfortheassessmentofsafetyandqualityoffoodproductsdevelopmentofsemi-automatedalternativemethodsnewmethodsarebasedongrowthandmetabolicactivityMicroscopicmethodsflowcytometryandcellsorting(FACS)BureauforInternationalResearchandTechnologyCooperation-AustriaImmunoassaysELISA(withdipsticktechnology),ImmunochromatographyFullyautomatedsystemsalreadyonthemarketNewrecombinantantibodiesandmolecularimprintingtechniquestoimprovesensitivityandversatilityBureauforInternationalResearchandTechnologyCooperation-AustriaImmunomagneticSeparationSpeedupenrichmentstepsNodetectionmethoditselfCombinationwithdifferentend-detectionmethodstoimprove-Speedandsensitivity-Samplethroughput-Userfriendliness-AutomationexpectedinthenextfutureBureauforInternationalResearchandTechnologyCooperation-AustriaNucleicacidbasedassayDirectlyorafteramplificationwithPCRDevelopmentofhomogenousassaysandautomationimprovesroutineuseandreducesfalsepositiveresultsafterPCRNewassayformatsunderdevelopmentBureauforInternationalResearchandTechnologyCooperation-AustriaBiosensorsandMicroarraysOn-lineoron-site,sensitive,low-cost,rapidmethodsforroutine-usePrototypesunderdevelopmentHavehighpotentialforautomationSimpleandportableequipmentcanbedesignedApplicationswithinqualityandprocesscontrol,controloffermentationprocesses,qualityandsafetycontrolofrawmaterialsandforHACCPmonitoringBureauforInternationalResearchandTechnologyCooperation-AustriaHygienemonitoringNeedtodeveloprealtimemethodstomonitorcleaningprocedureMethodsshouldbecheap,robustATPbioluminescence,Adenylatekinase,biosensorapplicationsmightbefurtherdevelopedBureauforInternationalResearchandTechnologyCooperation-AustriaMethodsImplementationInhousealternativemethodsforviablecellcountshygienemonitoringchemicalanalysisOutsourcingpathogentestingBureauforInternationalResearchandTechnologyCooperation-AustriaTheMarketforFoodDiagnosticsDefinitionsFoodAnalysis:allfoodtestingFoodDiagnostics:foodtestingwithrapidmethodsMicrobiologicalTesting:alltestingformicroorganismsFoodMicrobiologicalTesting:alltestingformicroorganismsinfoodandbeveragesBureauforInternationalResearchandTechnologyCooperation-AustriaTheMarketforFoodDiagnostics

SomefiguresFoodanalysisglobalmarket€1.1billionSharedbyabout50companiesUSAandEuropemakeupabout2/3€565millionglobalsalesforFoodmicrobiologicaltestsRapidmethods€115millionora20%totalmarketshareBureauTechnologySales($USmillions)MarketShare(%)ELISA/EIA7061DNA2522Impedance109FlowCytometry54ATP54Total115100BureauforInternationalResearchandTechnologyCooperation-Austria

FoodAnalysis(€million)

FoodDiagnostics(€million)%FoodDiagnostics(€million)Foodpathogens97,51315%Spoilage22037,517%Chemicalcontaminants5319,540%Processchemicals1201610%Total490,586

BureauforInternationalResearchandTechnologyCooperation-AustriaValidationAOACvalidationbodyintheU.S.NordValandMicroValaretheNordicandEuropeanbodiesProblem:lackofacommonvalidationprocedureInhibitsestablishmentofrapidmethodsBureauforInternationalResearchandTechnologyCooperation-AustriaConclusionsManynewmethodsandtechnologiesarebeingdevelopedIncreasedsamplethroughputandefficiencythroughautomationThemarketforrapidmethodsissmallPenetrationofrapidmethodshasbeenslowLackofacommonvalidationprocedureinhibitsacceptanceofnewmethodsTheincentivetotransfertechnologiesmightbetoolowBureauforInternationalResearchandTechnologyCooperation-AustriaRecommendationsMicroarraysandbiosensorsshouldbetechnologicallyevaluatedNewmarketopportunitieswithinqualityandprocesscontrolshouldbeexploredThedevelopmentofnewmethodsshouldalwaysincludeeffortstoimplementthenewmethodTheformationofaprivateservicesectorshouldbeencouragedBureauforInternationalResearchandTechnologyCooperation-AustriaBureauforInternationalResearchandTechnologyCooperation-AustriaSecondaryFoodProcessingDomesticFoodPreparationFoodretailing

FoodCateringPrimaryProductionSupplyofAgriculturalInputsPrimaryFoodProcessingFoodDistributionBureauforInternationalResearchandTechnologyCooperation-AustriaFoodSafetyManagementToolsBureauforInternationalResearchandTechnologyCooperation-Austria9、靜夜四無(wú)鄰鄰，荒居舊業(yè)業(yè)貧。。12月-2212月-22Friday,December23,202210、雨中中黃葉葉樹，，燈下下白頭頭人。。。02:10:3602:10:3602:1012/23/20222:10:36AM11、以我獨(dú)沈久久，愧君相見見頻。。12月-2202:10:3602:10Dec-2223-Dec-2212、故人江海別別，幾度隔山山川。。02:10:3602:10:3602:10Friday,December23,202213、乍見見翻疑疑夢(mèng)，，相悲悲各問問年。。。12月月-2212月月-2202:10:3702:10:37December23,202214、他鄉(xiāng)鄉(xiāng)生白白發(fā)，，舊國(guó)國(guó)見青青山。。。23十十二二月20222:10:37上上午02:10:3712月月-2215、比不了得得就不比，，得不到的的就不要。。。。十二月222:10上上午12月-2202:10December23,202216、行動(dòng)出成成果，工作作出財(cái)富。。。2022/12/232:10:3702:10:3723December202217、做前，能夠夠環(huán)視四周；；做時(shí)，你只只能或者最好好沿著以腳為為起點(diǎn)的射線線向前。。2:10:37上午2:10上上午02:10:3712月-229、沒沒有有失失敗敗，，只只有有暫暫時(shí)時(shí)停停止止成成功功??！。。12月月-2212月月-22Friday,December23,202210、很很多多事事情情努努力力了了未未必必有有結(jié)結(jié)果果，，但但是是不不努努力力卻卻什什么么改改變變也也沒沒有有。。。。02:10:3702:10:3702:1012/23/20222:10:37AM11、成功就是日日復(fù)一日那一一點(diǎn)點(diǎn)小小努努力的積累。。。12月-2202:10:3702:10Dec-2223-Dec-2212、世間成事，，不求其絕對(duì)對(duì)圓滿，留一一份不足，可可得無(wú)限完美美。。02:10:3702:10:3702:10Friday,December23,202213、不知香積寺寺，數(shù)里入云云峰。。12月-2212月-2202:10:3702:10:37December23,202214、意志堅(jiān)強(qiáng)的的人能把世界界放在手中像像泥塊一樣任任意揉捏。23十二月月20222:10:37上午02:10:3712月-2215、楚塞三湘接接，荊門九派派通。。。十二月222:10上上午12月-2202:10December23,202216、少年年十五五二十十時(shí)，，步行行奪得得胡馬馬