Chrysin-5-7-Dihydroxyflavone-DataSheet-生命科學試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEChrysinCat.No.:HY-14589CASNo.:480-40-0Synonyms:5,7-Dihydroxyflavone分?式:C??H??O?分?量:254.24作?靶點:EstrogenReceptor/ERR作?通路:Others儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數據體外實驗DMSO:≥100mg/mL(393.33mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.9333mL19.6665mL39.3329mL5mM0.7867mL3.9333mL7.8666mL10mM0.3933mL1.9666mL3.9333mL請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內使?，-20°C儲存時，請在1個?內使?。體內實驗請根據您的實驗動物和給藥?式選擇適當的溶解?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結果的可靠性，澄的儲備液可以根據儲存條件，適當保存；體內實驗的?作液，建議您現?現配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現沉淀、析出現象，可以通過加熱和/或超聲的?式助溶)1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(9.83mM);Clearsolution2.請依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(9.83mM);ClearsolutionBIOLOGICALACTIVITY?物活性Chrysin?種雌激素阻斷劑。IC50&Targetestrogen體外研究Chrysinismainlyfoundinpassionflowers,honey,andpropolisactsasapotentialtherapeuticandpreventiveagenttoinhibitproliferationandinvasionofvarioushumancancercells.AlthoughChrysinhasanti-carcinogeniceffectsinseveralcancers,littleisknownaboutitsfunctionalrolesinovariancancerwhichshowspoorprognosisandchemoresistancetotraditionaltherapeuticagents.Chrysininhibitsovariancancercellproliferationandinducedcelldeathbyincreasingreactiveoxygenspecies(ROS)productionandcytoplasmicCa2+levelsaswellasinducinglossofmitochondrialmembranepotential(MMP).ChrysinactivatesMAPKandPI3K/AKTpathwaysinES2andOV90cellsinconcentration-responseexperiments.ChrysinsuppressestumorgrowthbyregulatingcanonicalWntandnuclearfactorNF-κBsignalingcascadescancercells.ChrysinstimulatesthephosphorylationofAKTandP70S6KproteinsinbothES2andOV90cellscomparedtotheuntreatedcontrolcells.Inaddition,Chrysinactivatesthephospho-ERK1/2,p38,andJNKproteinsasmembersoftheMAPKpathwayintheovariancancercells[1].PROTOCOLCellAssay[1]Theproliferationassaysareconductedusingacellproliferationenzyme-linkedimmunosorbentassay(ELISA)5-bromo-2'-deoxyuridine(BrdU)kit.Briefly,ES2andOV90cellsareseededina96-wellplate,andthentreatedwithChrysin(0,5,10,20,50,and100μM)withorwithoutinhibitors(20μMLY294002,PI3K/AKT;10μMU0126,ERK1/2;10μMSP600125,JNK;and20μMSB203580,p38)inafinalvolumeof100μL/well.Aftera48-hincubation,10μMBrdUisaddedtothecellculture,followedbyanadditional2-hincubationat37°C.AfterlabelingthecellswithBrdU,theyarefixedandthenincubatedwiththeanti-BrdU-peroxidase(POD)workingsolutionfor90min.Theanti-BrdU-PODbindstotheBrdUincorporatedintonewlysynthesizedcellularDNAandtheseimmunecomplexesaredetectedbyanalyzingtheirreactionwiththe3,3',5,5'-tetramethylbenzidine(TMB)substrate.Theabsorbancevaluesofthereactionproductaremeasuredat370and492nmusinganELISAreader[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產品發(fā)表的科研?獻?ActaPharmSinB.2021Jan;11(1):143-155.?ClinSci.2021Jul20;CS20210571.?IntJMolMed.July13,2021.2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemE?CellCycle.2022Jan5;1-13.?RegenTher.13January2022.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].LimW,etal.ChrysinAttenuatesProgressionofOvarianCancerCellsbyRegulatingSignalingCascadesandMitochondrialDysfunction.JCellPhysiol.2017Aug17.McePdfHeightCautio