肥大細(xì)胞對THP-1巨噬細(xì)胞源性泡沫細(xì)胞膽固醇分配和流出的影響

肥大細(xì)胞對THP-1巨噬細(xì)胞源性泡

沫細(xì)胞膽固醇分配和流出的影響2021/4/261肥大細(xì)胞(MCs)一種參與炎癥免疫應(yīng)答反應(yīng)的炎癥細(xì)胞。胞漿內(nèi)含有大量異染性顆粒（組胺等）。受刺激時(shí)脫顆粒,釋放胞漿一系列可溶性介質(zhì)到細(xì)胞外

（compound48/80、oxLDL等可以刺激MCs脫顆粒)

。研究背景2021/4/262促動(dòng)脈粥樣硬化(As)形成

MCs加速oxLDL誘導(dǎo)平滑肌細(xì)胞泡沫化。

Compound48/80處理的新西蘭白兔和apoE-/-鼠主動(dòng)脈脂紋形成比對照組早，面積大，分布廣。2021/4/263HDLMetabolismand

ReverseCholesterolTransport膽固醇流出減少A-ICECECEFCFCLCATFCBileSR-BIA-IABCA1Mature

HDLNascent

HDLMC?ABC1=ATP-bindingcassetteprotein1;A-I=apolipoproteinA-I;

CE=cholesterylester;FC=freecholesterol;

LCAT=lecithin:cholesterolacyltransferase;

SR-BI=scavengerreceptorclassBI2021/4/264實(shí)驗(yàn)結(jié)果ABTheeffectofcompound48/80on

MCsdegranulation

MCswerestainedbytoluidineblue(×1000)

A，MCs；B，MCs+C48/80.

例數(shù)組胺含量（μg/ml）組胺釋放率（n）顆粒釋放液細(xì)胞裂解液（%）

50.340±0.0050.077±0.02181.53±4.51

MCshistaminereleasepercentage2021/4/265FormationofTHP-1macrophage-derivedfoamcells

CE/TCRatio:68.7%

HPLCanalysisofTHP-1macrophage-derivedfoamcellsOilredOstainingof

THP-1macrophage-derivedfoamcellsFC，freecholesterolCE，cholesterolesterFCCE2021/4/266

TheeffectofMCsontheaccumulationofcellcholesterolinTHP-1macrophage-derivedfoamcells

GroupsTCFCCEcontrol1092.3±65336.3±31756±60HDL3527.3±49a276.9±34250.4±17MCs891.3±62650.1±46b241.2±19bHDL3+MCs917.9±14c848.5±16c69.4±2

cC48/80807.3±68343.1±36464.2±13HDL3+C48/80314.3±10a253.2±1161.1±8Valuesarerepresentedas（）mgcholesterol/gprotein,(n=5).a:P<0.05vs.control,HDL3+MCsgroup;b:P<0.05vs.controlgroup;c:P<0.05vs.HDL3group.2021/4/267CholesterolstandardcontrolHDL3MCsHDL3+MCs

C48/80

HDL3+C48/80

ThechromatographypictureofcholesterolandcholesterolesterinTHP-1macrophage-derivedfoamcellsidentifiedbyhighperformanceliquidchromatography.2021/4/268TheeffectofMCsonthedistributionofcellfree

andesterifiedcholesterolinTHP-1macrophage-derivedfoamcells

control

(alone)=69.2%;withHDL3=47.5%;withMCs

=27.1%;withHDL3+MCs

=7.6%;withcompound48/80=57.5%;andwithcompound48/80+HDL3=19.4%.a:P<0.05vs.controlgroup;b:P<0.001vs.HDL3group.n=5.2021/4/269TheeffectofMCsoncholesteroleffluxfromTHP-1macrophage-derivedfoamcellsmediatedbyHDL3

control=2.89%;withHDL3=40.98%;withMCs

=2.91%;withHDL3+MCs

=26.92%;withcompound48/80=3.71%;andwithcompound48/80+HDL3=35.23%.

Thevaluesaremean±SD(n=5).**

P<0.01vs.HDL3+MCsgroup;#P<0.05vs.HDL3+MCsgroup;*

P<0.001vs.HDL3group.2021/4/26109、人的價(jià)值，在招收誘惑的一瞬間被決定。2023/2/32023/2/3Friday,February3,202310、低頭要有勇氣，抬頭要有低氣。2023/2/32023/2/32023/2/32/3/20234:29:20PM11、人總是珍惜為得到。2023/2/32023/2/32023/2/3Feb-2303-Feb-2312、人亂于心，不寬余請。2023/2/32023/2/32023/2/3Friday,February3,202313、生氣是拿別人做錯(cuò)的事來懲罰自己。2023/2/32023/2/32023/2/32023/2/32/3/202314、抱最大的希望，作最大的努力。03二月20232023/2/32023/2/32023/2/315、一個(gè)人炫耀什么，說明他內(nèi)心缺少什么。。二月232023/2/32023/2/32023/2/32/3/202316、業(yè)余生活要有意義，不要越軌。2023/2/32023/2/303February202317、一個(gè)人即使已登上頂峰，也仍要自強(qiáng)不息。2023/2/32023/2/32023/2/32023/2/3GAPDH(697bp)ABCA1(306bp)MarkercontrolHDL3MCsHDL3+MCsC48/80HDL3+C48/80*

The

effectof

MCsonABCA1mRNAexpressioninTHP-1macrophage-derivedfoamcells

AfterthetreatmentofMCs,nosignificantchangeswereshownintranscriptionlevelofcellABCA1comparedwithcontrolgroup.

Comparedwithcontrolgroup,*

P>0.05.2021/4/2612KDa3125

196A

12BSt1215%SDS[wt/vol]AGE

DegradationofHDL3andapoA-IbyMCs1,untreatedHDL3;2,MCs-treatedHDL3.2021/4/2613MCs能降解HDL3的apoA-Ⅰ；MCs能抑制HDL3介導(dǎo)的THP-1巨噬細(xì)胞源性泡沫細(xì)胞膽固醇流出，促使細(xì)胞內(nèi)游離膽固醇蓄積。結(jié)論2021/4/2614謝謝!2021/4/26159、人的價(jià)值，在招收誘惑的一瞬間被決定。2023/2/32023/2/3Friday,February3,202310、低頭要有勇氣，抬頭要有低氣。2023/2/32023/2/32023/2/32/3/20234:29:20PM11、人總是珍惜為得到。2023/2/32023/2/32023/2/3Feb-2303-Feb-2312、人亂于心，不寬余請。2023/2/32023/2/32023/2/3Friday,February3,202313、生氣是拿別人做錯(cuò)的事來懲罰自己。2023/2/32023/2/32023/2/32023/2/32/3/202314、抱最大的希望，作最大的努力。03二月